scholarly journals Highly sensitive gas chromatographic analysis of ethanol in whole blood, serum, urine, and fecal supernatants by the direct injection method

1997 ◽  
Vol 43 (6) ◽  
pp. 1003-1009 ◽  
Author(s):  
Albert Tangerman
Keyword(s):  
Blood Serum ◽  
Whole Blood ◽  
Direct Injection ◽  
Glass Beads ◽  
Injection Technique ◽  
Large Sample ◽  
Highly Sensitive ◽  
Gas Chromatographic Method ◽  
Ethanol Determination ◽  
Gas Chromatographic Separation

Abstract A highly sensitive, reproducible, and rapid gas chromatographic method for ethanol determination in various biological specimens (human whole blood, serum, urine, and fecal supernatants) was developed. The method involves direct injection of the biological specimen into the gas chromatograph, without any pretreatment. Contamination of the gas chromatographic column with nonvolatile material was prevented by the use of a glass liner in the injector. This liner, which acted as a precolumn, was partly filled with small glass beads. Injection was performed in between the glass beads. More than 50 injections of the various biological specimens could be done before the liner had to be replaced by a new one. This injection technique between glass beads allows direct injection of large sample volumes up to 10 μL without disturbing the gas chromatographic separation. Injection of these large sample volumes made the method very sensitive. The detection limit for ethanol amounted to 0.1 mg/L (2 μmol/L) when using an injection volume of 5 μL. Attention has also been paid to simultaneously monitoring ethanol, methanol, acetaldehyde, and acetone in blood and urine of control subjects.

Antiheparin Activity of Human Serum and Platelet Factor 4

1973 ◽  
Vol 30 (01) ◽  
pp. 093-105 ◽  
Author(s):  
C.H.J Sear ◽  
L Poller ◽  
F.R.C Path
Keyword(s):  
Molecular Weight ◽  
Ionic Strength ◽  
Blood Serum ◽  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Gel Filtration ◽  
Normal Serum ◽  
Platelet Factor ◽  
Mean Values ◽  
Antiheparin Activity

SummaryThe antiheparin activity of normal serum has been studied by comparing the antiheparin activities of sera obtained from normal whole blood, platelet-rich plasma and platelet-’free’ plasma with a purified platelet extract during differential isoelectric precipitation and by gel filtration chromatography.The mean values for the activity of PRP-serum and PFP-serum were 106% (S.D. 11) and 10% (S.D. 3) of untreated whole blood respectively. The activity of whole blood serum, PRP serum and whole blood serum plus platelet extract precipitated under identical physical conditions, i.e. pH 7.0, I =0.008, indicating that the activities of the three samples are probably associated with PF4. PF4 precipitated from human platelet extract at pH 4.0, but this is probably due to the difference in the two biochemical environments investigated, i.e. serum and platelet extract.The gel filtration experiments revealed striking similarities between the major antiheparin activities of serum and platelet extract. At physiological pH and ionic strength both activities were associated with high molecular weight material, but at physiological pH and elevated ionic strength both activities behaved as much smaller entities of molecular weight between 25,000 and 30,000 daltons and it seems very likely that both activities are associated with the same molecule, i.e. PF4.

The relationship between CSF and serum findings when diagnosed with syphilis

1935 ◽  
Vol 31 (1) ◽  
pp. 138-138
Author(s):  
F. Каug
Keyword(s):  
Cerebrospinal Fluid ◽  
Blood Serum ◽  
Specific Reaction ◽  
Highly Sensitive ◽  
Positive Serum ◽  
The Relationship

The authors studied the question of whether a highly sensitive and strictly specific reaction of blood serum flocculation can be an auxiliary tool in assessing positive reactions of cerebrospinal fluid. A study of 1400 cases has proven that a positive serum flocculation reaction invariably accompanies a special colloidal reaction (Goldsolreaktion).

Drugs as Probes of Organ Function: Evaluation of the Hepatobiliary Axis Using Oral Rifampicin and Novel High Performance Liquid Chromatography

1987 ◽  
Vol 24 (1) ◽  
pp. 36-40 ◽  
Author(s):  
J D Berg ◽  
S Ruddock ◽  
R A C Allen-Narker ◽  
G V H Bradby ◽  
M Davis ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
High Performance ◽  
Direct Injection ◽  
Systemic Circulation ◽  
Function Evaluation ◽  
Injection Technique ◽  
Organ Function ◽  
Gilbert’S Syndrome ◽  
Gilbert's Syndrome ◽  
Concentration Curves

Investigation of the uptake and metabolism of drugs by organs such as the liver may allow assessment of specific aspects of organ function. Rifampicin, when orally administered, is transported into the hepatocyte from portal blood and thence passes, with its deacetylated metabolite, into the systemic circulation and into bile. This paper reports an investigation of the pharmacokinetics of a sub-therapeutic oral dose of rifampicin in healthy subjects, in patients with cirrhosis and in subjects with Gilbert's syndrome. The areas under the plasma concentration curves (AUC) in patients with cirrhosis were significantly greater than in healthy subjects. Subjects with Gilbert's syndrome had decreased AUCs compared with healthy subjects and were clearly distinguished from patients with cirrhosis. Rifampicin concentration in serum was measured by HPLC using a novel direct injection technique.

scholarly journals Simultaneous Measurement of 3-Chlorotyrosine and 3,5-Dichlorotyrosine in Whole Blood, Serum and Plasma by Isotope Dilution HPLC–MS-MS

2016 ◽  
Vol 40 (4) ◽  
pp. 264-271 ◽  
Author(s):  
Brian S. Crow ◽  
Jennifer Quiñones-González ◽  
Brooke G. Pantazides ◽  
Jonas W. Perez ◽  
W. Rucks Winkeljohn ◽  
...  
Keyword(s):  
Blood Serum ◽  
Isotope Dilution ◽  
Simultaneous Measurement ◽  
Whole Blood

Gas-Solid Chromatographic Procedures for Determining Acrylonitrile Monomer in Acrylonitrile-Containing Polymers and Food Simulating Solvents

1978 ◽  
Vol 61 (6) ◽  
pp. 1383-1388
Author(s):  
Margaret E Brown ◽  
Charles V Breder ◽  
Timothy P Mcneal
Keyword(s):  
Acetic Acid ◽  
Polymer Matrix ◽  
Chromatographic Method ◽  
Direct Injection ◽  
Migration Studies ◽  
Quantitation Limit ◽  
Food Simulant ◽  
Nitrogen Phosphorus Detector ◽  
Gas Chromatographic Method ◽  
Nitrogen Phosphorus

Abstract A gas chromatographic method is described for acrylonitrile monomer (AN), using a nitrogen/phosphorus detector. Procedures for the analysis of AN in 5 food simulants (water, 3% acetic acid, heptane, 50% ethanol, and 8% ethanol) as well as in the polymer matrix are included. The quantitation limit for direct injection of AN/food simulant solution is 0.04 ng AN/μl. AN-based polymers are dissolved in N,N-dimethylacetamide and injected directly. Residual AN in the polymer can be quantitated at the 0.5 ng/μl level. Results of migration studies are also presented.

Excretion of a Toxic Dose of Thallium

1990 ◽  
Vol 36 (8) ◽  
pp. 1506-1509 ◽  
Author(s):  
H A Chandler ◽  
G P R Archbold ◽  
J M Gibson ◽  
P O'Callaghan ◽  
J N Marks ◽  
...  
Keyword(s):  
Blood Serum ◽  
Whole Blood ◽  
Assisted Ventilation ◽  
Toxic Dose ◽  
Serum And Urine

Abstract We report a successfully treated case of severe thallium intoxication that required 95 days of assisted ventilation and 224 days of hospitalization. Monitoring of the patient for 500 days by measuring thallium in whole blood, serum, and urine is documented, and the role of the laboratory and utility of the measurements are considered.

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