Characterization of Myocardial Protein Composition in Dilated Cardiomyopathy by Two-Dimensional Gel Electrophoresis

1994 ◽  
Vol 15 (suppl D) ◽  
pp. 37-44 ◽  
Author(s):  
M. Knecht ◽  
V. Regitz-Zagrosek ◽  
K.-P. Pleissner ◽  
P. Jungblut ◽  
C. Steffen ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Gel Electrophoresis ◽  
Protein Composition ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Characterization of antithrombin III from human plasma by two-dimensional gel electrophoresis and capillary electrophoretic methods

2003 ◽  
Vol 24 (24) ◽  
pp. 4282-4290 ◽  
Author(s):  
Leopold Kremser ◽  
Andrea Brückner ◽  
Andrea Heger ◽  
Tom Grunert ◽  
Andrea Buchacher ◽  
...  
Keyword(s):  
Human Plasma ◽  
Gel Electrophoresis ◽  
Antithrombin Iii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Capillary Electrophoretic

Characterization of Enterobacter cloacae under phoxim stress by two-dimensional gel electrophoresis

2015 ◽  
Vol 20 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Dijun Zhang ◽  
Jiajie Xu ◽  
Weina He ◽  
Qianqian Tong ◽  
Liping Chen ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Identification of phagocytosis-associated surface proteins of macrophages by two-dimensional gel electrophoresis.

1982 ◽  
Vol 92 (2) ◽  
pp. 283-288 ◽  
Author(s):  
F D Howard ◽  
H R Petty ◽  
H M McConnell
Keyword(s):  
Cell Surface ◽  
Gel Electrophoresis ◽  
Protein Composition ◽  
Surface Protein ◽  
Surface Proteins ◽  
Two Dimensional ◽  
Cell Surface Protein ◽  
Two Dimensional Gel Electrophoresis ◽  
Reducing Conditions ◽  
Membrane Components

Two-dimensional PAGE (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell. 1977. Cell. 12:1133-1142) has been employed to assess the effects of antibody-dependent phagocytosis on the cell surface protein composition of RAW264 macrophages. Unilamellar phospholipid vesicles containing 1% dinitrophenyl-aminocaproyl-phosphatidylethanolamine (DNP-cap-PE) were used as the target particle. Macrophages were exposed to anti-DNP antibody alone, vesicles alone, or vesicles in the presence of antibody for 1 h at 37 degrees C. Cell surface proteins were then labeled by lactoperoxidase-catalyzed radioiodination at 4 degrees C. After detergent solubilization, membrane proteins were analyzed by two-dimensional gel electrophoresis. The resulting pattern of spots was compared to that of standard proteins. We have identified several surface proteins, not apparently associated with the phagocytic process, which are present either in a multichain structure or in several discretely charged forms. After phagocytosis, we have observed the appearance of two proteins of 45 and 50 kdaltons in nonreducing gels. In addition, we have noted the disappearance of a 140-kdalton protein in gels run under reducing conditions. These alterations would not be detected in the conventional one-dimensional gel electrophoresis. This evidence shows that phagocytosis leads to a modification of cell surface protein composition. Our results support the concept of specific enrichment and depletion of membrane components during antibody-dependent phagocytosis.

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