Multimarker method in the study of laboratory predictors of vascular wall elasticity as the basis for the development of a personalized approach to the treatment of socially significant diseases

Abstract Funding Acknowledgements Type of funding sources: None. Aim To investigate the role of markers of vascular inflammation, vitamin D, parathyroid hormone as predictors of increased pulse-wave velocity (PWV) and degenerative bone changes in postmenopausal women with arterial hypertension (AH). Methods 164 females were examined. Gr.1 included 42 healthy individuals, Gr.2 - 58 patients with AH and Gr.3 - 64 postmenopausal women with AH and osteoporosis. Parameters of blood pressure monitoring; PWV, osteodensitometry (T-Score); inflammatory markers: hsCRP, TNFα, homocysteine, IL-1β, 6, 8, endothelin-1; lipid profile parameters; sex and parathyroid hormones, vitamin D were measured. Results In Gr.3 excess levels of PWV, hsCRP, homocysteine, IL8, total cholesterol, LDL cholesterol, endothelin-1 and parathyroid hormone was detected with decrease in the level of sex hormones and vitamin D. Besides, negative correlations of T-Score with age, PWV, duration of menopause, IL-6, hsCRP were registered; positive correlations between PWV with IL6, LDL cholesterol, hsCRP, endothelin-1, DBP variability were found. The logistic regression method revealed the main markers that affect increase of PWV, such as hsCRP and endothelin-1.Rise of each marker by unit of measurement leads to increase in PWV by 1.3 times and 2.4%, respectively. In Gr.2 increase in PWV level of more than 12.05 m/s was associated with 3.8-fold increase in the risk of osteoporosis. In Gr.3 increase in PWV level on 1 m/s was associated with 6 fold increase in the risk of osteoporosis. Conclusions Elevated levels of PWV are associated with markers of inflammation, levels of parathyroid hormone, vitamin D, T-Score and may be part of the pathogenesis of the cardiovascular continuum in postmenopausal women, which will require an individual approach to the treatment of AH with comorbid metabolic disorders.

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Vitamin D Status and Its Seasonal Variations and Association With Parathyroid Hormone Concentration in Healthy Women in Riga

Medicina ◽

10.3390/medicina49070051 ◽

2013 ◽

Vol 49 (7) ◽

pp. 51

Author(s):

Aivars Lejnieks ◽

Anda Slaidina ◽

Agnis Zvaigzne ◽

Una Soboleva ◽

Gulsena Eivazova ◽

...

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Seasonal Variations ◽

Threshold Level ◽

Vitamin D Supplementation ◽

Reproductive Age ◽

Vitamin D Status ◽

Healthy Women ◽

Vitamin D Supplements

The aim of the study was to describe the vitamin D status and its seasonal variations in women living in Riga, Latvia, to examine an association between the concentrations of plasma 25-hydroxyvitamin D [25(OH)D] and parathyroid hormone (PTH), and to determine the threshold for plasma 25(OH)D above which there is no further suppression of PTH. Material and Methods. The data of 189 healthy Caucasian women were analyzed. The serum levels of 25(OH)D, PTH, and phosphorus were measured twice a year. All the participants were divided into 3 groups according to vitamin D supplementation and the reproductive status. Results. The overall mean level of 25(OH)D was 32.8 ng/mL with significantly lower levels being in winter when compared with those in summer (28.2 ng/mL vs. 37.5 ng/mL, respectively; P<0.05). PTH was negatively associated with 25(OH)D. A threshold level of plasma 25(OH)D above which no further suppression of PTH occurred was found to be 38 ng/mL. Postmenopausal women not taking vitamin D supplements and without exposure to sunlight had 25(OH)D deficiency in winter and summer (92% and 88%, respectively). The most significant seasonal fluctuations were seen in the women of the reproductive age not taking vitamin D supplements and without exposure to sunlight, of which 47% had 25(OH)D deficiency in summer and 69% in winter. Conclusions. An optimal concentration of 25(OH)D was found to be 38 ng/mL. According to this definition, 70.4% of all the healthy women were classified as vitamin D deficient in winter and 59.8% in summer. The highest proportion of vitamin D deficient individuals was found in the group representing the postmenopausal women not taking vitamin D supplements.

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Vitamin D status: effects on parathyroid hormone and 1,25-dihydroxyvitamin D in postmenopausal women

American Journal of Clinical Nutrition ◽

10.1093/ajcn/71.6.1577 ◽

2000 ◽

Vol 71 (6) ◽

pp. 1577-1581 ◽

Cited By ~ 128

Author(s):

Allan G Need ◽

Michael Horowitz ◽

Howard A Morris ◽

BE Christopher Nordin

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Vitamin D Status ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D

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REGULATORY HYPERPARATHYROIDISM IN A PIG BREED WITH VITAMIN D DEPENDENCY RICKETS

Acta Endocrinologica ◽

10.1530/acta.0.0920295 ◽

1979 ◽

Vol 92 (2) ◽

pp. 295-308 ◽

Cited By ~ 12

Author(s):

R. Wilke ◽

J. Harmeyer ◽

C. von Grabe ◽

R. Hehrmann ◽

R. D. Hesch

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Binding Assay ◽

Clinical Symptoms ◽

Plasma Concentrations ◽

Fold Increase ◽

Protein Binding Assay ◽

Deficiency Rickets ◽

Clinical Healing ◽

Immunoreactive Parathyroid Hormone

ABSTRACT A radioimmunoassay for porcine parathyroid hormone has been developed and applied to measure immunoreactive parathyroid hormone (PTH) in plasma of pigs with hereditary vitamin D dependency rickets (VDDR) (pseudovitamin D deficiency rickets). Levels of 25-hydroxycholecalciferol (25-(OH)-D3) in plasma were measured by a protein binding assay. Both plasma concentrations of PTH and 25-(OH)-D3 showed an approximately 4-fold increase compared to normal pigs. PTH levels increased with duration of the disease. Daily dosing of the animals with 1–4 μg of 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) reduced PTH concentrations and resulted in clinical healing. Iv administration of 10 μg of 25-(OH)-D3/day did not alter PTH concentrations nor the clinical symptoms. The results suggest that these animals suffer from regulatory hyperparathyroidism. The metabolic defect could be due to a failure of the kidney to convert 25-(OH)-D3 to 1,25-(OH)2-D3.

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Influence of the levels of vitamin D on T-score in HIP measured by DXA of colombian postmenopausal women

Endocrine Abstracts ◽

10.1530/endoabs.56.p164 ◽

2018 ◽

Author(s):

Richard Buendia ◽

Santiago Cardenas ◽

Monica Zambrano ◽

Andres Buendia ◽

Maria De Los Angeles Varon ◽

...

Keyword(s):

Vitamin D ◽

Postmenopausal Women ◽

T Score

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Abstract P037: Independent and Joint Associations of 25-Hydroxy Vitamin D and Parathyroid Hormone Levels With Cardiometabolic Biomarkers Among American White and Black Postmenopausal Women

Circulation ◽

10.1161/circ.139.suppl_1.p037 ◽

2019 ◽

Vol 139 (Suppl_1) ◽

Author(s):

Jin Xia ◽

Wanzhu Tu ◽

JoAnn E Manson ◽

Hongmei Nan ◽

Aladdin H Shadyab ◽

...

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Hormone Levels ◽

25 Hydroxy Vitamin D ◽

Joint Associations

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Diurnal rhythm of plasma 1,25-dihydroxyvitamin D and vitamin D-binding protein in postmenopausal women: relationship to plasma parathyroid hormone and calcium and phosphate metabolism

Acta Endocrinologica ◽

10.1530/eje.0.1460635 ◽

2002 ◽

pp. 635-642 ◽

Cited By ~ 55

Author(s):

L Rejnmark ◽

AL Lauridsen ◽

P Vestergaard ◽

L Heickendorff ◽

F Andreasen ◽

...

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Diurnal Rhythm ◽

Plasma Levels ◽

Diurnal Variations ◽

Vitamin D Binding Protein ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D ◽

Pattern Of Variation

OBJECTIVE: Diurnal variations in plasma levels of 1,25-dihydroxyvitamin D (1,25(OH)(2)D) have previously only been investigated in young individuals, and these studies have failed to demonstrate a diurnal rhythm. We have studied whether plasma levels of 1,25(OH)(2)D and vitamin D-binding protein (DBP) vary in a diurnal rhythm in postmenopausal women. METHODS: Blood and urine were sampled with 2- and 4-h intervals in order to assess diurnal variations in plasma levels of 1,25(OH)(2)D, DBP and parathyroid hormone (PTH), as well as in plasma levels and urinary excretion rates of calcium and phosphate. Additionally, the free 1,25(OH)(2)D index was calculated (the molar ratio of 1,25(OH)(2)D to DBP). RESULTS: Plasma 1,25(OH)(2)D exhibited a diurnal rhythm (P<0.01) with a nadir in the morning (99+/-12 pmol/l), followed by a rapid increase to a plateau during the day (113+/-13 pmol/l, i.e. 14% above nadir level; P=0.005). A similar pattern of variation was found in plasma levels of DBP with peak levels 15% above nadir levels (P<0.01). The free 1,25(OH)(2)D index did not vary in a diurnal rhythm. PTH and plasma levels and urinary excretions of calcium and phosphate exhibited a diurnal pattern of variation. The diurnal rhythm of DBP was correlated with the rhythm of 1,25(OH)(2)D (r=0.47, P<0.01) and plasma albumin (r=0.76, P<0.01). Moreover, the rhythm of plasma calcium and PTH varied inversely (r=-0.36, P=0.02). CONCLUSIONS: With the disclosure of a diurnal rhythm of total plasma 1,25(OH)(2)D, all major hormones and minerals related to calcium homeostasis have now been shown to exhibit diurnal variations. In clinical studies, the diurnal variations of 1,25(OH)(2)D and DBP must be considered, i.e. blood sampling must be standardised according to the time of day.

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Vitamin D and parathyroid hormone in relation to bone mineral density in postmenopausal women

Vojnosanitetski pregled ◽

10.2298/vsp1203243v ◽

2012 ◽

Vol 69 (3) ◽

pp. 243-248 ◽

Cited By ~ 4

Author(s):

Marina Vuceljic ◽

Olivera Ilic-Stojanovic ◽

Milica Lazovic ◽

Mirko Grajic

Keyword(s):

Bone Mineral Density ◽

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Bone Mineral ◽

Epidemiological Data ◽

Significant Negative Correlation ◽

Vitamin D Insufficiency ◽

Mineral Density ◽

Hydroxyvitamin D

Background/Aim. Despite vitamin D insufficiency being widely reported, in Serbia the epidemiological data lack information regarding vitamin D status in the sera of postmenopausal women. The aim of this study was to establish the prevalence of inadequate serum 25-hydroxyvitamin D [25(OH)D] concentrations in postmenopausal Serbian women with seasonal variations of 25(OH)D, in relation to parathyroid hormone (PTH) and bone mineral density (BMD). Methods. A total of 95 postmenopausal women, mean age 65.1 ? 9.08 years, were examined. Measurements of 25(OH)D and PTH were performed both in the winter and the summer period, using electrochemiluminiscence immunoassays. BMD (g/cm2) was measured by the dualenergy x-Ray absortimetry (DXA) method on the spine and hip areas. Results. A decreased value of vitamin D (< 75 nmol/L) in 88.4% of postmenopausal women and an elevated level of PTH (> 65 pg/mL) in 25.3% of the cases were found. Elevated PTH varied individually, but was mostly increased if 25(OH)D was equal or lower than 37.6 nmol/L. 25(OH)D insufficiency was found in winter in 94.5% and in summer in 80% of the cases (p < 0.01). The mean of the PTH was higher (p < 0.05) in winter than in summer. A significant negative correlation between 25(OH)D and PTH (p < 0.001) was proved. Correlation between 25(OH)D and PTH with BMD at lumbar spine was established in the whole group, but at the femoral neck in women aged over 65 years (p < 0.05). Conclusion. Our results showed a high prevalence of vitamin D insufficiency (88.4%) among postmenopausal women. The levels of 25(OH)D and PTH changed significantly according to the season.

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Effect of Vitamin D Intake on Seasonal Variations in Parathyroid Hormone Secretion in Postmenopausal Women

New England Journal of Medicine ◽

10.1056/nejm198912283212602 ◽

1989 ◽

Vol 321 (26) ◽

pp. 1777-1783 ◽

Cited By ~ 244

Author(s):

Elizabeth A. Krall ◽

Nadine Sahyoun ◽

Saul Tannenbaum ◽

Gerard E. Dallal ◽

Bess Dawson-Hughes

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Postmenopausal Women ◽

Seasonal Variations ◽

Hormone Secretion ◽

Vitamin D Intake

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Effects of oral and transdermal estrogen therapies on circulating cytokines and chemokines in postmenopausal women with hysterectomy

Acta Endocrinologica ◽

10.1530/eje-09-0063 ◽

2009 ◽

Vol 161 (2) ◽

pp. 267-273 ◽

Cited By ~ 13

Author(s):

Toshiyuki Yasui ◽

Ayako Saijo ◽

Hirokazu Uemura ◽

Toshiya Matsuzaki ◽

Naoko Tsuchiya ◽

...

Keyword(s):

Postmenopausal Women ◽

Adhesion Molecule ◽

Vascular Inflammation ◽

Estrogen Therapy ◽

Intercellular Adhesion Molecule ◽

Serum Concentrations ◽

Intercellular Adhesion Molecule 1 ◽

Cytokines And Chemokines ◽

Markers Of Inflammation ◽

Circulating Levels

ObjectiveThe aim of the present study was to determine the different effects of oral estrogen therapy (ET) and transdermal ET on changes in circulating levels of cytokines and chemokines in relationship to changes in markers of inflammation in postmenopausal women with hysterectomy.MethodsFifty-five postmenopausal women with hysterectomy were randomly assigned in open, parallel-group fashion to an oral ET group and a transdermal ET group. Serum levels of cytokines and chemokines were simultaneously measured using a multiplexed human cytokine assay. Serum concentrations of high-sensitive C-reactive protein, soluble vascular cell adhesion molecule-1, soluble intercellular adhesion molecule-1, and E-selectin were measured as vascular inflammation markers.ResultsBoth oral ET and transdermal ET significantly decreased serum interleukin (IL)-7 concentrations at 12 months (P=0.020 and P=0.015 respectively). Transdermal ET decreased serum concentrations of IL-8, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-1β (P=0.05, P=0.019, and P=0.029), but oral ET increased IL-8 level (P=0.025). There were significant differences in percentage changes in IL-8 and MIP-1β between the oral and transdermal ET groups. Oral ET significantly decreased E-selectin level after 12 months.ConclusionTransdermal ET reduces circulating levels of IL-8, MCP-1, and MIP-1β, while both oral ET and transdermal ET reduce circulating level of IL-7.

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