Isolation and characterization of the novel oil-degrading strain Kosakonia cowanii IUMR B67 and expression of the degradation enzyme

2020 ◽  
Author(s):  
Jianjun Ren ◽  
Zhenzhu Wang ◽  
Dongze Niu ◽  
Huhetaoli ◽  
Xu Huang ◽  
...  
Keyword(s):  
Food Waste ◽  
Lipase Activity ◽  
Edible Oils ◽  
Oil Degradation ◽  
Basic Salt ◽  
Lipase Gene ◽  
Base Pairs ◽  
Triacylglycerol Lipase ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Abstract Oils in food waste can pollute the environment and negatively affect human health. Biodegradation is a promising method for disposing of waste edible oils. In this study, an oil-degrading bacterium was isolated from kitchen waste for efficient degradation of edible oils. Its growth and oil degradation characteristics were investigated in basic salt medium with edible oils as the sole carbon and energy source; the triacylglycerol lipase gene (EC 3.1.1.3) was cloned and expressed in Escherichia coli. A novel oil-degrading bacterium assigned as IUMR B67 was successfully isolated. Morphological and molecular analyses revealed that strain IUMR B67 belongs to Kosakonia cowanii. After 144 h of incubation, the oil degradation rate at 37°C was 95.80%. Optimal conditions for IUMR B67 were recorded at 37°C and 0.1% NaCl with 0.1% ammonium sulfate supplementation. The lipase gene of strain IUMR B67 was determined to be 912 base pairs, and the lipase activity of the expressed protein was 3.02 U/mL, which was significantly higher than the control (P < 0.05). Overall, Kosakonia cowanii IUMR B67 is a novel edible oil-degrading strain that can hydrolyze oil via its lipase activity, which may be useful in the disposal of oils and oily food waste.

scholarly journals Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

2021 ◽  
Author(s):  
Jin Gao ◽  
Jie Ming ◽  
Meng Xu ◽  
Xinge Fu ◽  
Liang-Feng Duan ◽  
...  
Keyword(s):  
Degradation Rate ◽  
High Efficiency ◽  
Jiaozhou Bay ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Single Factor ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Oil Concentration ◽  
Diesel Oil Degradation

AbstractIn the recent 50 years, marine oil spills had resulted in severe environmental pollution problems worldwide. In this study, 12 petroleum-degrading strains named MJ1 to MJ12, which can use diesel oil as the sole carbon source for growth, were isolated from the seawater in Jiaozhou Bay, China. Strain MJ4 has the highest diesel-degrading rate which is up to 26.54% in 5 days with the diesel oil concentration of 10 g/L. According to the BLAST research, 16SrRNA sequence of MJ4 showed 99% similarity to Bacillus megaterium strain. Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp. MJ4. Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28 °C, pH of 8.8, diesel oil concentration of 25 g/L, P/N ratio of 0.56, nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L, respectively. A nonlinear regression equation of diesel oil degradation rate and pH, temperature, P/N ratio was obtained. The model predicted the maximum degradation rate of 72.21% with temperature of 28 °C, pH of 8.88, P/N ratio of 0.31, respectively.

scholarly journals Isolation and Characterization of Beneficial Bacteria from Food Process Wastes

2021 ◽  
Vol 9 (6) ◽  
pp. 1156
Author(s):  
A-Leum Kim ◽  
Seunghye Park ◽  
Yoon-Kyoung Hong ◽  
Ji-Hwan Shin ◽  
Se-Hwan Joo
Keyword(s):  
Food Waste ◽  
High Throughput Sequencing ◽  
Microbial Community Composition ◽  
Waste Recycling ◽  
Organic Content ◽  
Plant Growth Promoting Bacteria ◽  
Acetic Acid Production ◽  
Process Waste ◽  
Isolation And Characterization ◽  
Plant Growth Promoting

Significant quantities of food waste are accumulated globally on an annual basis, with approximately one-third of the food produced (equivalent to 1.3 billion tons of food) being wasted each year. A potential food waste recycling application is its utilization as a soil conditioner or fertilizer, whereby it increases the soil organic content and microbial biomass. This study evaluated the effectiveness of food waste as a microbial resource by analyzing the microbial community composition and isolating plant growth-promoting bacteria (PGPB) in food waste obtained from various sources. High-throughput sequencing identified 393 bacterial operational taxonomic units in the food process waste (FPW) samples. Moreover, the results showed that Firmicutes was abundant in the waste samples, followed by Bacteroidetes and Proteobacteria. A total of 92 bacteria were isolated from FPW. Moreover, the cultivable strains isolated from FPW belonged to the genus Bacillus, followed by Streptomyces and Proteus. Six isolated bacteria exhibited beneficial traits, including indole acetic acid production, antifungal resistance and extracellular lysis. FPW is a valuable microbial resource for isolation of PGPB, and its use as a fertilizer may enable a reduction in chemical fertilizer usage, thereby mitigating the corresponding adverse environmental impacts on sustainable crop development.

scholarly journals Identification of a triacylglycerol lipase gene family inCandida deformans: molecular cloning and functional expression

Yeast ◽  
2003 ◽  
Vol 20 (3) ◽  
pp. 233-248 ◽  
Author(s):  
Fr�d�ric Bigey ◽  
Karine Tuery ◽  
Daisy Bougard ◽  
Jean-Marc Nicaud ◽  
Guy Moulin
Keyword(s):  
Gene Family ◽  
Molecular Cloning ◽  
Functional Expression ◽  
Lipase Gene ◽  
Triacylglycerol Lipase

scholarly journals Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3

2019 ◽  
Vol 51 (2) ◽  
pp. 170-178
Author(s):  
Tao Peng ◽  
Qing-Kun Chen ◽  
Jing-Sheng Lun ◽  
Amit Pratush ◽  
Guang-Ming Xiong ◽  
...  
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

2005 ◽  
Vol 251 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Li Yang ◽  
Yu-hua Zhao ◽  
Bing-xin Zhang ◽  
Ching-Hong Yang ◽  
Xin Zhang
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

scholarly journals Lipid droplets modulate proteostasis, SQST-1/SQSTM1 dynamics, and lifespan in C. elegans

2021 ◽  
Author(s):  
Anita Kumar ◽  
Joslyn Mills ◽  
Wesley Parker ◽  
Joshua Leitão ◽  
Celeste Ng ◽  
...  
Keyword(s):  
Translational Regulation ◽  
Lipid Droplets ◽  
Dependent Manner ◽  
Lipase Gene ◽  
Triacylglycerol Lipase ◽  
Selective Autophagy ◽  
C Elegans ◽  
Protein Ubiquitination ◽  
Model Of Alzheimer’S Disease ◽  
Age Related

Abstract The ability of organisms to live long depends largely on the maintenance of proteome stability via proteostatic mechanisms including translational regulation, protein chaperoning and degradation machineries. In several long-lived Caenorhabditis elegans strains, such as insulin/IGF-1 receptor daf-2 mutants, enhanced proteostatic mechanisms are accompanied by elevated intestinal lipid stores, but the role of lipid droplets in longevity has remained obscure. Here, while determining the regulatory network of the selective autophagy receptor SQST-1/SQSTM1, we unexpectedly uncovered a novel role for lipid droplets in proteostasis and longevity. Using an unbiased genomewide RNAi screening approach, we identified several SQST-1 modulators, including proteins found on lipid droplets and those prone to aggregate with age. SQST-1 accumulated on lipid droplets when autophagy was inhibited, suggesting that lipid droplets may serve a role in facilitating selective autophagy. Expansion of intestinal lipid droplets by silencing the conserved cytosolic triacylglycerol lipase gene atgl-1/ATGL enhanced autophagy, and extended lifespan in an HSF-1/HSF1-dependent and CDC-48/VCP-dependent manner. Silencing atgl-1 mitigated the age-related accumulation of SQST-1 and reduced overall ubiquitination of proteins. Reducing atgl-1 also improved proteostasis in a nematode model of Alzheimer’s disease. Subcellular analyses revealed that lipid droplets unexpectedly harbor more ubiquitinated proteins than the cytosol. Accordingly, low lipid droplet levels exacerbated the proteostatic collapse when autophagy or proteasome function was compromised. Altogether, our study uncovers a key role for lipid droplets in C. elegans as a proteostatic mediator that reduces protein ubiquitination, facilitates autophagy, and promotes longevity.

Isolation and characterization of an electrochemically active and cyanide-degrading bacterium isolated from a microbial fuel cell

RSC Advances ◽  
2014 ◽  
Vol 4 (69) ◽  
pp. 36458 ◽  
Author(s):  
Feng Ya-li ◽  
Wang Wei-da ◽  
Tang Xin-hua ◽  
Li Hao-ran ◽  
Du Zhuwei ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Electrochemically Active
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