The recruitment of bacterial communities by the plant root system changed by acid mine drainage pollution in soils

2020 ◽  
Vol 367 (15) ◽  
Author(s):  
Yang Li ◽  
Liang Yuan ◽  
Sheng Xue ◽  
Bingjun Liu ◽  
Gang Jin
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Mine Drainage ◽  
Plant Root ◽  
Bacterial Community Composition ◽  
Cellular Processes ◽  
Acid Mine ◽  
Functional Bacteria ◽  
Plant Root System

ABSTRACT This study aims to better understand the relationship between the response to acid mine drainage (AMD) stress of tolerant plants and changes in root-related bacterial communities. In this study, reed stems were planted in AMD-polluted and unpolluted soils, and high-throughput sequencing was conducted to analyze the bacterial community composition in the soil, rhizosphere, rhizoplane and endosphere. The results showed that the effect of AMD pollution on root-associated bacterial communities was greater than that of rhizo-compartments. Proteobacteria were dominant across the rhizo-compartments between treatments. The microbiomes of unpolluted treatments were enriched by Alphaproteobacteria and Betaproteobacteria and depleted in Gammaproteobacteria ranging from the rhizoplane into the endosphere. However, the opposite trend was observed in the AMD pollution treatment, namely, Gammaproteobacteria were enriched, and Alphaproteobacteria and Deltaproteobacteria were mostly depleted. In addition, endophytic microbiomes were dominated by Comamonadaceae and Rhodocyclaceae in the unpolluted treatment and by Enterobacteriaceae in the AMD-polluted soils. PICRUSt showed that functional categories associated with membrane transport, metabolism and cellular processes and signaling processes were overrepresented in the endosphere of the AMD-polluted treatment. In conclusion, our study reveals significant variation in bacterial communities colonizing rhizo-compartments in two soils, indicating that plants can recruit functional bacteria to the roots in response to AMD pollution.

scholarly journals Dynamics of Bacterial Communities Mediating the Treatment of an As-Rich Acid Mine Drainage in a Field Pilot

2018 ◽  
Vol 9 ◽  
Author(s):  
Elia Laroche ◽  
Corinne Casiot ◽  
Lidia Fernandez-Rojo ◽  
Angélique Desoeuvre ◽  
Vincent Tardy ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Communities ◽  
Mine Drainage ◽  
Acid Mine

scholarly journals Diversity and spatiotemporal dynamics of bacterial communities: physicochemical and other drivers along an acid mine drainage

2014 ◽  
Vol 90 (1) ◽  
pp. 247-263 ◽  
Author(s):  
Aurélie Volant ◽  
Odile Bruneel ◽  
Angélique Desoeuvre ◽  
Marina Héry ◽  
Corinne Casiot ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Communities ◽  
Mine Drainage ◽  
Spatiotemporal Dynamics ◽  
Acid Mine

scholarly journals Biofilm Bacterial Community Structure in Streams Affected by Acid Mine Drainage

2009 ◽  
Vol 75 (11) ◽  
pp. 3455-3460 ◽  
Author(s):  
Gavin Lear ◽  
Dev Niyogi ◽  
Jon Harding ◽  
Yimin Dong ◽  
Gillian Lewis
Keyword(s):  
Community Structure ◽  
Acid Mine Drainage ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Mine Drainage ◽  
Iron Hydroxide ◽  
Acidic Ph ◽  
Dissolved Metals ◽  
Acid Mine ◽  
The Impact

ABSTRACT We examined the bacterial communities of epilithic biofilms in 17 streams which represented a gradient ranging from relatively pristine streams to streams highly impacted by acid mine drainage (AMD). A combination of automated ribosomal intergenic spacer analysis with multivariate analysis and ordination provided a sensitive, high-throughput method to monitor the impact of AMD on stream bacterial communities. Significant differences in community structure were detected among neutral to alkaline (pH 6.7 to 8.3), acidic (pH 3.9 to 5.7), and very acidic (pH 2.8 to 3.5) streams. DNA sequence analysis revealed that the acidic streams were generally dominated by bacteria related to the iron-oxidizing genus Gallionella, while the organisms in very acidic streams were less diverse and included a high proportion of acidophilic eukaryotes, including taxa related to the algal genera Navicula and Klebsormidium. Despite the presence of high concentrations of dissolved metals (e.g., Al and Zn) and deposits of iron hydroxide in some of the streams studied, pH was the most important determinant of the observed differences in bacterial community variability. These findings confirm that any restoration activities in such systems must focus on dealing with pH as the first priority.

scholarly journals Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China

2008 ◽  
Vol 11 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Yu Yang ◽  
Wuyang Shi ◽  
Minxi Wan ◽  
Yanfei Zhang ◽  
Lihong Zou ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Communities ◽  
Mine Drainage ◽  
Gansu Province ◽  
Copper Mine ◽  
Acid Mine

scholarly journals Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

2015 ◽  
Vol 81 (15) ◽  
pp. 4874-4884 ◽  
Author(s):  
Weimin Sun ◽  
Tangfu Xiao ◽  
Min Sun ◽  
Yiran Dong ◽  
Zengping Ning ◽  
...  
Keyword(s):  
Microbial Community ◽  
Acid Mine Drainage ◽  
High Throughput Sequencing ◽  
Southwest China ◽  
Mine Drainage ◽  
Redox Potentials ◽  
Content Type ◽  
Acid Mine ◽  
Abandoned Coal Mines ◽  
S Cycling

ABSTRACTLocated in southwest China, the Aha watershed is continually contaminated by acid mine drainage (AMD) produced from upstream abandoned coal mines. The watershed is fed by creeks with elevated concentrations of aqueous Fe (total Fe > 1 g/liter) and SO42−(>6 g/liter). AMD contamination gradually decreases throughout downstream rivers and reservoirs, creating an AMD pollution gradient which has led to a suite of biogeochemical processes along the watershed. In this study, sediment samples were collected along the AMD pollution sites for geochemical and microbial community analyses. High-throughput sequencing found various bacteria associated with microbial Fe and S cycling within the watershed and AMD-impacted creek. A large proportion of Fe- and S-metabolizing bacteria were detected in this watershed. The dominant Fe- and S-metabolizing bacteria were identified as microorganisms belonging to the generaMetallibacterium,Aciditerrimonas,Halomonas,Shewanella,Ferrovum,Alicyclobacillus, andSyntrophobacter. Among them,Halomonas,Aciditerrimonas,Metallibacterium, andShewanellahave previously only rarely been detected in AMD-contaminated environments. In addition, the microbial community structures changed along the watershed with different magnitudes of AMD pollution. Moreover, the canonical correspondence analysis suggested that temperature, pH, total Fe, sulfate, and redox potentials (Eh) were significant factors that structured the microbial community compositions along the Aha watershed.

scholarly journals Physiological, Genomic and Transcriptomic Analyses Reveal the Adaptation Mechanisms of Acidiella bohemica to Extreme Acid Mine Drainage Environments

2021 ◽  
Vol 12 ◽  
Author(s):  
Shu-ning Ou ◽  
Jie-Liang Liang ◽  
Xiao-min Jiang ◽  
Bin Liao ◽  
Pu Jia ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Acid Mine Drainage ◽  
Single Molecule ◽  
High Throughput Sequencing ◽  
Mine Drainage ◽  
Acid Stress ◽  
Sequencing Technology ◽  
Metabolic Potential ◽  
Acid Mine ◽  
Adaptation Mechanisms

Fungi in acid mine drainage (AMD) environments are of great concern due to their potentials of decomposing organic carbon, absorbing heavy metals and reducing AMD acidity. Based on morphological analysis and ITS/18S high-throughput sequencing technology, previous studies have provided deep insights into the diversity and community composition of fungi in AMD environments. However, knowledge about physiology, metabolic potential and transcriptome profiles of fungi inhabiting AMD environments is still scarce. Here, we reported the physiological, genomic, and transcriptomic characterization of Acidiella bohemica SYSU C17045 to improve our understanding of the physiological, genomic, and transcriptomic mechanisms underlying fungal adaptation to AMD environments. A. bohemica was isolated from an AMD environment, which has been proved to be an acidophilic fungus in this study. The surface of A. bohemica cultured in AMD solutions was covered with a large number of minerals such as jarosite. We thus inferred that the A. bohemica might have the potential of biologically induced mineralization. Taking advantage of PacBio single-molecule real-time sequencing, we obtained the high-quality genome sequences of A. bohemica (50 Mbp). To our knowledge, this was the first attempt to employ a third-generation sequencing technology to explore the genomic traits of fungi isolated from AMD environments. Moreover, our transcriptomic analysis revealed that a series of genes in the A. bohemica genome were related to its metabolic pathways of C, N, S, and Fe as well as its adaptation mechanisms, including the response to acid stress and the resistance to heavy metals. Overall, our physiological, genomic, and transcriptomic data provide a foundation for understanding the metabolic potential and adaptation mechanisms of fungi in AMD environments.

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