scholarly journals Role of uropathogenic Escherichia coli outer membrane protein T in pathogenesis of urinary tract infection

2015 ◽  
Vol 73 (3) ◽  
Author(s):  
Xiao Long He ◽  
Qin Wang ◽  
Liang Peng ◽  
Ya-Rong Qu ◽  
Santhosh Puthiyakunnon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Uropathogenic Escherichia Coli

scholarly journals Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli – Implications for urinary tract infection

2010 ◽  
Vol 49 (3) ◽  
pp. 59-66 ◽  
Author(s):  
Lovisa Svensson ◽  
Mirjana Poljakovic ◽  
Susanne Säve ◽  
Nicola Gilberthorpe ◽  
Thomas Schön ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Nitrosative Stress ◽  
Uropathogenic Escherichia Coli

scholarly journals ANTIGEN OMP (OUTER MEMBRANE PROTEIN) salmonella typhi FAGA LOKAL YANG IMUNODOMINAN DAN SPESIFIK TERHADAP ANTIBODI PENDERITA DEMAM TIFOID

2018 ◽  
Vol 13 (3) ◽  
pp. 109
Author(s):  
J. Nugraha ◽  
Rahayu Anggraini
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Membrane Protein ◽  
Typhoid Fever ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Phage Type ◽  
The Other ◽  
Control Group

Background of this research is that diagnostics of typhoid fever is still a health problem. Widal test, which is the mostly used test in Indonesia, frequently gives false positive results and is not reliable in endemic areas. On the other hand, the other confirmation test, bloodculture, is not sensitive and often give false negative results. OMP (Outer Membrane Protein) is known as a specific part of Salmonellatyphi and fragments of OMP still exist in the patient’s body since early infection until 2–3 weeks thereafter. In this study parts of OMPwhich react specifically with sera of typhoid fever in Indonesia were searched. These specific parts will then be developed as a diagnostickit for typhoid fever. Using Western Blot method, part of OMP will be searched, which is specifically react with sera of typhoid feverpatients in Indonesia. OMP derived from local phage type isolated in Indonesia was used. This OMP was separated with SDS-PAGE 12%and incubated with pooled sera of typhoid fever patients, and sera of control group, that is from Dengue haemorrhagic fever patientsand urinary tract infection with E. coli. Extraction of OMP was done by the method of Matsuyama. Contrary, this research failed to find a particular part of OMP which react specifically with sera of typhoid fever patients. There are certain parts of OMP which react also with sera of DHF & urinary tract infection patients. Our finding was different with the results from Malaysia, where it is reported thatantigen OMP 52 kD react specifically there. In order to develop a diagnostic tool for typhoid fever, we should consider another possiblespecific antigen other rather than using OMP.

Role of Toxins of Uropathogenic Escherichia coli in Development of Urinary Tract Infection

2018 ◽  
Vol 21 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Saber Soltani ◽  
Amir Emamie ◽  
Mahsa Dastranj ◽  
Abbas Farahani ◽  
Abolfazl Davoodabadi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Uropathogenic Escherichia Coli

scholarly journals Roles of OmpX, an outer membrane protein on virulence and flagellar expression in uropathogenic Escherichia coli

2021 ◽  
Author(s):  
Hidetada Hirakawa ◽  
Kazutomo Suzue ◽  
Ayako Takita ◽  
Wataru Kamitani ◽  
Haruyoshi Tomita
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Epithelial Cells ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Parent Strain ◽  
Antimicrobial Agents ◽  
Uropathogenic Escherichia Coli ◽  
Renal Cells

Uropathogenic Escherichia coli (UPEC) is a major pathogen that causes urinary tract infection (UTI). This bacterium adheres to and internalizes within urinary tract cells, where it aggregates and subsequently forms biofilm-like multicellular colonies that protect UPEC from antimicrobial agents and the host’s immune system. Here we show that OmpX, an outer-membrane protein, plays a role in the pathogenesis of UPEC in renal cells. Deletion of ompX decreased bacterial internalization and aggregation within kidney epithelial cells, and also impaired the colonization of mice urinary tracts, but the ompX mutant still adhered to the epithelial cells with a similar level to the parent strain. FlhD, the master regulator of flagella-related genes, had a low expression level in the ompX mutant compared to the parent strain, and the ompX mutant exhibited defective motility due to lower flagellar production than the parent strain. The fliC mutant, which lacks flagella, exhibited lower levels of bacterial internalization and aggregation than the parent strain. Additional deletion of ompX in the fliC mutant did not further decrease bacterial internalization. These combined results suggest that OmpX contributes to flagellar production in UPEC, then sustains UPEC virulence associated with bacterial internalization and aggregation within urinary tract cells and colonization in the urinary tract.

scholarly journals CAPSULE PRODUCTION PATTERN OF UROPATHOGENIC ESCHERICHIA COLI OF URINARY TRACT INFECTION PATIENTS IN KIRKUK HOSPITALS

2020 ◽  
Vol 17 (35) ◽  
pp. 621-627
Author(s):  
May Ali Hussien UMRAN ◽  
Sumaya Najim AL-KHATEEB
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clinical Manifestations ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Intestinal Pathogens ◽  
Production Pattern ◽  
Living Organisms

The bacterium Escherichia coli is one of the best free-living organisms studied in depth. It is a surprisingly diverse species, since some strains of E. coli live in the intestine of animals as harmless commensals, while other distinct genotypes, such as an enteropathogenic or enterohemorrhagic E. coli, for example, cause morbidity and death marked as human intestinal pathogens. The purpose of this study was to develop and validate a PCR assay for a known and suspected uropathogenic E. coli virulence factor (kpsMT) gene region to determine the distribution of the gene and its role in the development of clinical diseases of the urinary system. A total of 25 urine samples were collected from patients with urinary tract infection (UTI) at Azadi and Kirkuk hospitals in the city of Kirkuk, Iraq. Samples of both genders and different ages were collected from patients with suspected urinary tract infection according to the clinical manifestations and symptoms diagnosed by the examining physician. The samples were cultured and positive samples were subjected to the IMViC test to identify E. coli bacteria and subsequently identified using the Vitek 2 compact system. Among 25 samples, 24 (96%) showed positive results for bacterial cultural growth. Of these, 17 (68%) were identified as Escherichia coli. Of the total of 17 isolates, 14 from patients with mild urinary tract infection, and 3 from patients with Urosepsis. The kpsMT gene was present in 14 isolates (82.3%), including 11 (78.5%) isolates from patients with mild urinary tract infection, and 3 (100%) isolates from patients with Urosepsis. It was concluded that Escherichia coli is the most prevalent in urine tract infection samples. Due to the abundance of the kpsMT gene in uropathogenic Escherichia coli (UPEC), this gene plays an important role in developing UTI if it is not treated correctly and quickly; mild cases of UTI can turn into Urosepsis.

scholarly journals Complete Genome Sequences of Seven Uropathogenic Escherichia coli Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Belle M. Sharon ◽  
Amber Nguyen ◽  
Amanda P. Arute ◽  
Neha V. Hulyalkar ◽  
Vivian H. Nguyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Postmenopausal Women ◽  
Complete Genome ◽  
Recurrent Urinary Tract Infection ◽  
Uropathogenic Escherichia Coli ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infection (UTI). This disease disproportionately affects women and frequently develops into recurrent UTI (rUTI) in postmenopausal women. Here, we report the complete genome sequences of seven UPEC isolates obtained from the urine of postmenopausal women with rUTI.

Virulence properties of uropathogenic Escherichia coli isolated from children with urinary tract infection in Korea

2018 ◽  
Vol 40 (6) ◽  
pp. 625-634 ◽  
Author(s):  
Dong Ho Kim ◽  
Bindu Subhadra ◽  
Hee Young Kang ◽  
Kyungho Woo ◽  
Jaeseok Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Uropathogenic Escherichia Coli ◽  
Virulence Properties
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