scholarly journals PHYSIOLOGICAL CHARACTERIZATION OF POLAR Tn5-INDUCED ISOLEUCINE-VALINE AUXOTROPHS IN ESCHERICHIA COLI K-12: EVIDENCE FOR AN INTERNAL PROMOTER IN THE ilvOGEDA OPERON

Genetics ◽  
1979 ◽  
Vol 93 (2) ◽  
pp. 309-319
Author(s):  
Claire M Berg ◽  
Karen J Shaw ◽  
Joyce Vender ◽  
Maryla Borucka-Mankiewicz
Keyword(s):  
Escherichia Coli ◽  
Enzyme Activity ◽  
Transposable Element ◽  
Feeding Behavior ◽  
Enzyme Activities ◽  
Internal Promoter ◽  
Physiological Characterization ◽  
Growth Requirements ◽  
K 12

ABSTRACT The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior, and enzyme activity. Mutants defective in ilvA, ilvC, ilvD and ilvE were found. Mutations in ilvE were not completely polar on ilvD and ilvA enzyme activities (that is, ilvE mutants possessed a low constitutive level of expression of the enzymes coded by ilvD and ilvA), while mutations in ilvD were completely polar on ilvA enzyme activity. The data suggest that there is an internal promoter between the sites of Tn5 insertion in ilvE and ilvD.

Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12

1994 ◽  
Vol 13 (1) ◽  
pp. 35-49 ◽  
Author(s):  
Ho-Ching Tiffany Tsui ◽  
Hon-Chiu Eastwood Leung ◽  
Malcolm E. Winkler
Keyword(s):  
Escherichia Coli ◽  
Insertion Mutation ◽  
K 12

scholarly journals Physical characterization of the ilvHI operon of Escherichia coli K-12.

1981 ◽  
Vol 147 (3) ◽  
pp. 797-804 ◽  
Author(s):  
C H Squires ◽  
M De Felice ◽  
S R Wessler ◽  
J M Calvo
Keyword(s):  
Escherichia Coli ◽  
Physical Characterization ◽  
K 12

Molecular characterization of ilvC specialized transducing phages of Escherichia coli K-12

1983 ◽  
Vol 191 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Norman W. Baylor ◽  
Arthur L. Williams ◽  
Nathan Cofie
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
K 12

IMMOBILIZATION OF AMYLASE PRODUCING BACTERIA (BACILLUS SUBTILIS) FROM SOIL OF WESTERN HIMALAYAN REGION SOLAN, HIMACHAL PRADESH, INDIA

2021 ◽  
pp. 109-115
Author(s):  
ARUN KUMAR ◽  
POONAM KUMARI ◽  
KASAHUN GUDETA ◽  
JM JULKA
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Physicochemical Properties ◽  
Bacterial Strain ◽  
Enzyme Activities ◽  
Enzyme Assay ◽  
Himachal Pradesh ◽  
Genus Bacillus ◽  
Western Himalayan Region

Objective: The paper aimed to immobilize amylase producing bacterial strain on a suitable matrix and characterization of its physicochemical properties so that much amount of amylase could be produced to be applied in different industries. Methods: Bacterial colonies were sub-cultured from samples collected from soil in freshly prepared dishes containing starch agar by dot method using sterile inoculating needles from which five different bacteria belonged to genus Bacillus were isolated and assigned as A1, A2, A3, A4, and A5. Results: It was found that A1 displayed the highest enzyme activity of 17.89 IU/ml with enzyme assay of 0.83 mg/ml and the bacterium was identified to be Bacillus subtilis. A5 displayed 10.13 IU/ml with protein contents of 0.11 mg/ml indicated that A1 possess the highest enzyme activities which were categorized under Bacillus and protein contents and A5 showed less amount of enzyme activities and protein contents as compared to other. Conclusion: The bacteria which were produced much amount of enzyme activities identified as Bacillus subtilis and recommended and have been recommended to be cultured for the production of amylase enzyme.

scholarly journals Characterization of the specific pyruvate transport system in Escherichia coli K-12.

1987 ◽  
Vol 169 (1) ◽  
pp. 380-385 ◽  
Author(s):  
V J Lang ◽  
C Leystra-Lantz ◽  
R A Cook
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
K 12
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