scholarly journals IDENTIFICATION OF A GENETIC ELEMENT THAT CONTROLS THE ORGAN-SPECIFIC EXPRESSION OF Adh1 IN MAIZE

Genetics ◽  
1981 ◽  
Vol 98 (2) ◽  
pp. 357-378
Author(s):  
James C Woodman ◽  
Michael Freeling
Keyword(s):  
Dna Sequences ◽  
Primary Root ◽  
Leucine Incorporation ◽  
Reciprocal Effect ◽  
Specific Expression ◽  
Cis Acting ◽  
Quantitative Expression ◽  
Close Proximity ◽  
Organ Specific ◽  
Organ Specific Expression

ABSTRACT Allozyme balances serve as markers of quantitative behavior of electrophoretically distinguishable alleles. By the use of ADH Set I allozyme balances, it is demonstrated that all Adh1-S/Adh1-F individuals from more than 20 diverse S/F families exhibit a reciprocal correlation between Adh1 quantitative behavior in two maize organs: the scutellum and primary root. Within an electrophoretic mobility class, the Adh1 allele that is relatively underexpressed in the scutellum is relatively overexpressed in the primary root, and vice versa. Segregation tests prove that this "reciprocal effect" is the property of a cis-acting site that is closely linked to or within the Adh1 structural gene, and it is not affected by diverse genetic backgrounds. Immunological and [3H]-leucine incorporation experiments establish that Adh1 quantitative variants differ in ADH1·ADH1 synthetic rates in the anaerobic primary root. The reciprocal-effect phenomenon suggests that the cis-acting loci controlling Adh1 quantitative expression in each respective organ are at least in close proximity, or may share common DNA sequences. We discuss the possibility that the reciprocal-effect locus is a regulatory component of the Adh1 cistron.

scholarly journals Genome-Wide Identification and Expression Profiling of the BZR Transcription Factor Gene Family in Nicotiana benthamiana

2021 ◽  
Vol 22 (19) ◽  
pp. 10379
Author(s):  
Xuwei Chen ◽  
Xinyang Wu ◽  
Shiyou Qiu ◽  
Hongying Zheng ◽  
Yuwen Lu ◽  
...  
Keyword(s):  
Stress Resistance ◽  
Nicotiana Benthamiana ◽  
Expression Patterns ◽  
Basic Research ◽  
Drought Treatment ◽  
Specific Expression ◽  
Cis Acting ◽  
Genome Wide ◽  
Organ Specific ◽  
Organ Specific Expression

Brassinazole-resistant (BZR) family genes encode plant-specific transcription factors (TFs), play essential roles in the regulation of plant growth and development, and have multiple stress-resistance functions. Nicotiana benthamiana is a model plant widely used in basic research. However, members of the BZR family in N. benthamiana have not been identified, and little is known about their function in abiotic stress. In this study, a total of 14 BZR members were identified in the N. benthamiana genome, which could be divided into four groups according to a phylogenetic tree. NbBZRs have similar exon-intron structures and conserved motifs, and may be regulated by cis-acting elements such as STRE, TCA, and ARE, etc. Organ-specific expression analysis showed that NbBZR members have different and diverse expression patterns in different tissues, and most of the members are expressed in roots, stems, and leaves. The analysis of the expression patterns in response to different abiotic stresses showed that all the tested NbBZR members showed a significant down-regulation after drought treatment. Many NbBZR genes also responded in various ways to cold, heat and salt stress treatments. The results imply that NbBZRs have multiple functions related to stress resistance.

scholarly journals Organ-Specific Expression of Maize Adh1 Is Altered After a Mu Transposon Insertion

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 469-477
Author(s):  
Che-Hong Chen ◽  
Karen K Oishi ◽  
Barbara Kloeckener-Gruissem ◽  
Michael Freeling
Keyword(s):  
Dna Sequences ◽  
Genomic Sequence ◽  
Male Gametophyte ◽  
Transcriptional Start Site ◽  
Start Site ◽  
Anaerobic Conditions ◽  
Specific Expression ◽  
Transposon Insertion ◽  
Organ Specific ◽  
Organ Specific Expression

ABSTRACT A new, unstable, organ-specific Adh1 mutant was isolated from a Robertson's mutator line by germinating kernels under partial anaerobic conditions. Families of kernels which showed segregation of a conditional anaerobic lethal phenotype were identified. One mutant, Adh1-3F1124, was shown to express approximately 6% normal levels of ADH1 in seed and anaerobically treated seedlings but expresses normal levels of ADH1 in pollen, the male gametophyte. The ADH1 polypeptide encoded by the mutant allele was found to be indistinguishable from that encoded by the Adh1-3F progenitor but its message levels were lower in seed and seedlings. Robertson's mutator lines are known to carry Mu transposons that cause increased mutation rates. Genomic Southern analysis of Adh1-3F1124 and Adh1-3F showed the presence of a 1.85 kbp insertion at the 5′ region of Adh1. Comparison of the DNA sequences revealed that a Mu1-like element was inserted 31 bp 5′ from the transcriptional start site of Adh1-3F1124gene. The insertion of the Mu element creates an additional TATA box by duplicating the 9 bp genomic sequence—ATATAAATC—at the site of insertion. Consequently, there are two potentially functional TATA sequences, separated by the 1.85 kbp Mu element, 5′ to the transcriptional start site. It is not yet understood how such an arrangement alters the organspecific expression of Adh1.

Organ specific expression of ras oncoproteins during growth and development of the rat

1986 ◽  
Vol 70 (1) ◽  
Author(s):  
Takeo Tanaka ◽  
Noriaki Ida ◽  
Hiroko Shimoda ◽  
Chiaki Waki ◽  
DennisJ. Slamon ◽  
...  
Keyword(s):  
Growth And Development ◽  
Specific Expression ◽  
Organ Specific ◽  
Organ Specific Expression
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