scholarly journals Genome-Wide Identification and Expression Profiling of the BZR Transcription Factor Gene Family in Nicotiana benthamiana

2021 ◽  
Vol 22 (19) ◽  
pp. 10379
Author(s):  
Xuwei Chen ◽  
Xinyang Wu ◽  
Shiyou Qiu ◽  
Hongying Zheng ◽  
Yuwen Lu ◽  
...  
Keyword(s):  
Stress Resistance ◽  
Nicotiana Benthamiana ◽  
Expression Patterns ◽  
Basic Research ◽  
Drought Treatment ◽  
Specific Expression ◽  
Cis Acting ◽  
Genome Wide ◽  
Organ Specific ◽  
Organ Specific Expression

Brassinazole-resistant (BZR) family genes encode plant-specific transcription factors (TFs), play essential roles in the regulation of plant growth and development, and have multiple stress-resistance functions. Nicotiana benthamiana is a model plant widely used in basic research. However, members of the BZR family in N. benthamiana have not been identified, and little is known about their function in abiotic stress. In this study, a total of 14 BZR members were identified in the N. benthamiana genome, which could be divided into four groups according to a phylogenetic tree. NbBZRs have similar exon-intron structures and conserved motifs, and may be regulated by cis-acting elements such as STRE, TCA, and ARE, etc. Organ-specific expression analysis showed that NbBZR members have different and diverse expression patterns in different tissues, and most of the members are expressed in roots, stems, and leaves. The analysis of the expression patterns in response to different abiotic stresses showed that all the tested NbBZR members showed a significant down-regulation after drought treatment. Many NbBZR genes also responded in various ways to cold, heat and salt stress treatments. The results imply that NbBZRs have multiple functions related to stress resistance.

scholarly journals Impact of IL-1 signalling on experimental uveitis and arthritis

2012 ◽  
Vol 71 (5) ◽  
pp. 753-760 ◽  
Author(s):  
Stephen R Planck ◽  
April Woods ◽  
Jenna S Clowers ◽  
Martin J Nicklin ◽  
James T Rosenbaum ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Eye Disease ◽  
Negative Regulators ◽  
Specific Expression ◽  
Local Responses ◽  
Intraocular Injection ◽  
Inflammatory Eye Disease ◽  
Pathogenesis Related ◽  
Organ Specific ◽  
Organ Specific Expression

BackgroundUveitis, or inflammatory eye disease, is a common extra-articular manifestation of many systemic autoinflammatory diseases involving the joints. Anakinra (recombinant interleukin (IL)-1 receptor antagonist (Ra)) is an effective therapy in several arthritic diseases; yet, few studies have investigated the extent to which IL-1 signalling or IL-1Ra influences the onset and/or severity of uveitis.ObjectiveTo seek possible links between arthritis and uveitis pathogenesis related to IL-1 signalling.MethodsThe eyes of IL-1Ra-deficient BALB/c mice were monitored histologically and by intravital videomicroscopy to determine if uveitis developed along with the expected spontaneous arthritis in ankles and knees. Expression levels of IL-1R and its negative regulators (IL-1Ra, IL-1RII, IL-1RAcP and single Ig IL-1R-related molecule) in eye and joint tissues were compared. Differences in uveitis induced by intraocular injection of lipopolysaccharide (LPS) in mice lacking IL-1R or IL-1Ra were assessed.ResultsDeficiency in IL-1Ra predisposes to spontaneous arthritis, which is exacerbated by previous systemic LPS exposure. The eye, however, does not develop inflammatory disease despite the progressive arthritis or LPS exposure. Organ-specific expression patterns for IL-1Ra and negative regulators of IL-1 activity were observed that appear to predict predisposition to inflammation in each location in IL-1Ra knockout mice. The eye is extremely sensitive to locally administered LPS, and IL-1Ra deficiency markedly exacerbates the resulting uveitis.ConclusionThis study demonstrates that IL-1Ra plays an important role in suppressing local responses in eyes injected with LPS and that there is discordance between murine eyes and joints in the extent to which IL-1Ra protects against spontaneous inflammation.

scholarly journals IDENTIFICATION OF A GENETIC ELEMENT THAT CONTROLS THE ORGAN-SPECIFIC EXPRESSION OF Adh1 IN MAIZE

Genetics ◽  
1981 ◽  
Vol 98 (2) ◽  
pp. 357-378
Author(s):  
James C Woodman ◽  
Michael Freeling
Keyword(s):  
Dna Sequences ◽  
Primary Root ◽  
Leucine Incorporation ◽  
Reciprocal Effect ◽  
Specific Expression ◽  
Cis Acting ◽  
Quantitative Expression ◽  
Close Proximity ◽  
Organ Specific ◽  
Organ Specific Expression

ABSTRACT Allozyme balances serve as markers of quantitative behavior of electrophoretically distinguishable alleles. By the use of ADH Set I allozyme balances, it is demonstrated that all Adh1-S/Adh1-F individuals from more than 20 diverse S/F families exhibit a reciprocal correlation between Adh1 quantitative behavior in two maize organs: the scutellum and primary root. Within an electrophoretic mobility class, the Adh1 allele that is relatively underexpressed in the scutellum is relatively overexpressed in the primary root, and vice versa. Segregation tests prove that this "reciprocal effect" is the property of a cis-acting site that is closely linked to or within the Adh1 structural gene, and it is not affected by diverse genetic backgrounds. Immunological and [3H]-leucine incorporation experiments establish that Adh1 quantitative variants differ in ADH1·ADH1 synthetic rates in the anaerobic primary root. The reciprocal-effect phenomenon suggests that the cis-acting loci controlling Adh1 quantitative expression in each respective organ are at least in close proximity, or may share common DNA sequences. We discuss the possibility that the reciprocal-effect locus is a regulatory component of the Adh1 cistron.

scholarly journals A common molecular basis to the convergent evolution of the selfing syndrome in Capsella

2019 ◽  
Author(s):  
Natalia Joanna Woźniak ◽  
Christian Kappel ◽  
Cindy Marona ◽  
Lothar Altschmied ◽  
Barbara Neuffer ◽  
...  
Keyword(s):  
Convergent Evolution ◽  
Evolutionary Biology ◽  
Expression Patterns ◽  
Network Connectivity ◽  
Specific Expression ◽  
Organ Specific ◽  
Self Fertilization ◽  
Organ Specific Expression ◽  
Comparative Genetic Mapping ◽  
Selfing Syndrome

AbstractWhether, and to what extent, phenotypic evolution follows predictable genetic paths, remains an important question in evolutionary biology. Convergent evolution of similar characters provides a unique opportunity to address this question. The transition to selfing and the associated changes in flower morphology are among the most prominent examples of repeated evolution in plants. Yet, to date no studies have directly compared the extent of similarities between convergent adaptations to selfing. In this study, we take advantage of the independent transitions to self-fertilization in the genus Capsella to test the existence of genetic and developmental constraints imposed on flower evolution in the context of the selfing syndrome. While C. rubella and C. orientalis have emerged independently, both have evolved almost identical flower characters. Not only the evolutionary outcome is identical but, in both cases, the same developmental strategies underlie the convergent reduction of flower size. This has been associated with convergent evolution of gene-expression changes. The transcriptomic changes common to both selfing lineages are enriched in genes with low-network connectivity and with organ-specific expression patterns. Comparative genetic mapping also indicates that, at least in the case of petal size evolution, these similarities are largely caused by mutations at the same loci. Together, these results suggest that the limited availability of low-pleiotropy paths predetermine closely related species to similar evolutionary outcomes.

scholarly journals Genome‑wide identification and characterization of Transcription Factors of Basic Leucine Zipper Family in Malus domestica

2016 ◽  
Author(s):  
Zhengrong Zhang ◽  
Haoran Cui ◽  
Shanshan Xing ◽  
Xin Liu ◽  
Xuesen Chen ◽  
...  
Keyword(s):  
Leucine Zipper ◽  
Regulation Of Gene Expression ◽  
Phylogenetic Groups ◽  
Basic Leucine Zipper ◽  
Specific Expression ◽  
Promoter Sequences ◽  
Genome Wide ◽  
Organ Specific ◽  
Organ Specific Expression ◽  
Splicing Patterns

AbstractBasic leucine zipper proteins (bZIP) contain a basic DNA-binding region and a leucine zipper region, acting as transcriptional factors in regulation of gene expression exclusively in eukaryotes. In this investigation, total 116 bZIP members were identified in apple genome and mapped on all 17 chromosomes with various densities as M.bZIPs. All these members were divided into six groups according to the phylogenetic relationship combining with bZIPs from rice and Arabidopsis. Investigating gene structure of M.bZIPs, five splicing patterns of intron were found in the DNA-binging region with no splicing position and splicing positions at different nucleotide of codons or different positions. Analyzing of protein structure of M.bZIPs, twenty-five motifs were identified with certain characteristic in different phylogenetic groups. To predict dimerization of leucine zipper region, the key positions of amino acid in heptad(s) were investigated. The results showed that most M.bZIPs may form hetero-dimer or homo-dimer and some M.bZIPs may form both. Expression experiment revealed that M.bZIP genes have organ-specific expression and widely expressed in flowers, leaves, and fruits. To investigate the response of M.bZIPs to abiotic stresses, the promoter sequences of randomly selected M.bZIP genes were analyzed. Cis-acting elements related to multiple stresses were found existing widely in promoter sequences. Quantitative real-time PCR results further demonstrated that the expression of some M.bZIP genes were quite sensitive to exogenous abscisic acid and osmotic treatments.

scholarly journals Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shaofei Rao ◽  
Xinyang Wu ◽  
Hongying Zheng ◽  
Yuwen Lu ◽  
Jiejun Peng ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Catharanthus Roseus ◽  
Nicotiana Benthamiana ◽  
Expression Profiles ◽  
Plant Immunity ◽  
Expression Patterns ◽  
Gene Expression Profiles ◽  
Phylogenetic Groups ◽  
Specific Expression ◽  
Cis Acting

Abstract Background The Catharanthus roseus RLK1-like kinase (CrRLK1L) is a subfamily of the RLK gene family, and members are sensors of cell wall integrity and regulators of cell polarity growth. Recent studies have also shown that members of this subfamily are involved in plant immunity. Nicotiana benthamiana is a model plant widely used in the study of plant-pathogen interactions. However, the members of the NbCrRLK1L subfamily and their response to pathogens have not been reported. Results In this study, a total of 31 CrRLK1L members were identified in the N. benthamiana genome, and these can be divided into 6 phylogenetic groups (I-VI). The members in each group have similar exon-intron structures and conserved motifs. NbCrRLK1Ls were predicted to be regulated by cis-acting elements such as STRE, TCA, ABRE, etc., and to be the target of transcription factors such as Dof and MYB. The expression profiles of the 16 selected NbCrRLK1Ls were determined by quantitative PCR. Most NbCrRLK1Ls were highly expressed in leaves but there were different and diverse expression patterns in other tissues. Inoculation with the bacterium Pseudomonas syringae or with Turnip mosaic virus significantly altered the transcript levels of the tested genes, suggesting that NbCrRLK1Ls may be involved in the response to pathogens. Conclusions This study systematically identified the CrRLK1L members in N. benthamiana, and analyzed their tissue-specific expression and gene expression profiles in response to different pathogens and two pathogens associated molecular patterns (PAMPs). This research lays the foundation for exploring the function of NbCrRLK1Ls in plant-microbe interactions.

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