scholarly journals Role for up-regulated ganglioside biosynthesis and association of Src family kinases with microdomains in retinoic acid-induced differentiation of F9 embryonal carcinoma cells

Glycobiology ◽  
2005 ◽  
Vol 15 (7) ◽  
pp. 687-699 ◽  
Author(s):  
Takashige Sato ◽  
Asif Mohammad Zakaria ◽  
Satoshi Uemura ◽  
Atsushi Ishii ◽  
Yoshiko Ohno-Iwashita ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Src Family Kinases ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Induced Differentiation ◽  
Ganglioside Biosynthesis ◽  
F9 Embryonal Carcinoma Cells

scholarly journals Bone morphogenetic proteins-2 and -4 are involved in the retinoic acid-induced differentiation of embryonal carcinoma cells.

1992 ◽  
Vol 3 (2) ◽  
pp. 189-196 ◽  
Author(s):  
M B Rogers ◽  
V Rosen ◽  
J M Wozney ◽  
L J Gudas
Keyword(s):  
Retinoic Acid ◽  
Bone Morphogenetic Proteins ◽  
Transforming Growth Factor Beta ◽  
Embryonal Carcinoma ◽  
Transforming Growth Factor ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Induced Differentiation ◽  
F9 Embryonal Carcinoma Cells

Bone morphogenetic proteins-2 and -4 (BMPs-2 and -4) are transforming growth factor beta-related proteins that can induce bone formation in vivo. We observed that the level of endogenous BMP-2 mRNA increased an average of 11-fold on differentiation of F9 embryonal carcinoma cells into parietal endoderm after treatment with retinoic acid (RA) and cAMP, whereas the message for the closely related BMP-4 decreased 12-fold after this treatment. Therefore, the effects of exogenous recombinant BMP-2 protein on the RA-induced differentiation of F9 embryonal carcinoma cells were investigated. BMP-2 addition altered the growth and morphology of RA-treated but not untreated cells. Moreover, the abundance of several messages was affected by exogenous BMP-2 treatment. Notably, the BMP-2 and -4 messages themselves were reduced by the addition of exogenous BMP-2. The observations suggest that RA, which is known to affect bone morphogenesis, may regulate the osteoinductive proteins, BMP-2 and -4. Furthermore, BMP-2 and -4 may be involved in preimplantation embryogenesis.

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