P-180 Bisphenols are present in culture media used for ART and cell culture

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C Vignault ◽  
A Togola ◽  
A Desmarchais ◽  
O Téteau ◽  
V Maillard ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bisphenol A ◽  
Solid Phase ◽  
Culture Media ◽  
Oocyte Quality ◽  
Isotopic Dilution ◽  
Bisphenol S ◽  
Registration Number ◽  
On Line

Abstract Study question Do plastic laboratory consumables and cell culture media used in human ART contain bisphenols? Summary answer Human embryo development media contained bisphenols close to the nanomolar concentration range while no release of bisphenols by plastic consumables was detected under routine conditions. What is known already The deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concerns regarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART, however it might have already been replaced by its structural analogs, including bisphenol S (BPS). Study design, size, duration Seventeen plastic consumables and 18 cell culture and ART media were assessed for the presence of bisphenols. Participants/materials, setting, methods Ten different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P, and Z) were measured using an isotopic dilution according to an on-line solid phase extraction / liquid chromatography/mass spectrometry method. Main results and the role of chance While all the plastic consumables of this study did contain bisphenols, none of them did release bisphenols under routine conditions. Moreover, 16 of the 18 cell culture and ART media assessed contained bisphenols, including 8 among the 10 media used in human ART. Five human ART media exhibited bisphenol concentrations higher than 0.8 nM and reached up to 3.2 nM (799 ng/L). Limitations, reasons for caution Further studies are required to investigate a greater number of ART media to identify less potentially harmful ones, in terms of bisphenol content. Wider implications of the findings As BPS has already been reported to impair oocyte quality at nanomolar concentrations, its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, there has been no regulation of these compounds in the ART context. Trial registration number Not applicable

P–180 Bisphenols are present in culture media used for ART and cell culture

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C Vignault ◽  
A Togola ◽  
A Desmarchais ◽  
O Téteau ◽  
V Maillard ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bisphenol A ◽  
Solid Phase ◽  
Culture Media ◽  
Oocyte Quality ◽  
Isotopic Dilution ◽  
Bisphenol S ◽  
Registration Number ◽  
On Line

Abstract Study question Do plastic laboratory consumables and cell culture media used in human ART contain bisphenols? Summary answer Human embryo development media contained bisphenols close to the nanomolar concentration range while no release of bisphenols by plastic consumables was detected under routine conditions. What is known already The deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concerns regarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART, however it might have already been replaced by its structural analogs, including bisphenol S (BPS). Study design, size, duration Seventeen plastic consumables and 18 cell culture and ART media were assessed for the presence of bisphenols. Participants/materials, setting, methods Ten different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P, and Z) were measured using an isotopic dilution according to an on-line solid phase extraction / liquid chromatography/mass spectrometry method. Main results and the role of chance While all the plastic consumables of this study did contain bisphenols, none of them did release bisphenols under routine conditions. Moreover, 16 of the 18 cell culture and ART media assessed contained bisphenols, including 8 among the 10 media used in human ART. Five human ART media exhibited bisphenol concentrations higher than 0.8 nM and reached up to 3.2 nM (799 ng/L). Limitations, reasons for caution Further studies are required to investigate a greater number of ART media to identify less potentially harmful ones, in terms of bisphenol content. Wider implications of the findings: As BPS has already been reported to impair oocyte quality at nanomolar concentrations, its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, there has been no regulation of these compounds in the ART context. Trial registration number Not applicable

Automated On-Line Noninvasive Optical Glucose Monitoring in a Cell Culture System

2002 ◽  
Vol 56 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Byungjo Jung ◽  
Seungjun Lee ◽  
In Hong Yang ◽  
Theresa Good ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Glucose Concentration ◽  
Culture System ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture System ◽  
Cell Culture Media ◽  
Line Measurement ◽  
On Line ◽  
Absorbance Spectra

Current techniques for monitoring glucose concentration during cell culture are invasively performed using an off-line measurement system. Even though in situ or ex situ analyte sensors have been tried for on-line measurement, they suffer from difficulties including the inability to keep them sterile, their limited lifetime, and their lack of stability. In this research an alternative optical noninvasive on-line monitoring system based on near-infrared absorbance spectroscopy was developed to measure glucose concentration of cell culture media in a rotary cell culture system. The system included an automatic sampling component controlled by a LabVIEW program and a high-throughput fiber coupling component connected to a Fourier transform near-infrared spectrometer. The effectiveness of the system was investigated via noninvasive on-line measurement of glucose absorbance spectra in the cell culture media during T-cell culture. Glucose absorbance spectra were collected in the spectral range of 2.0 to 2.5 μm. Partial least-squares regression was employed to build a successful multivariate calibration model. The standard error of prediction and mean percent error for glucose were 7.7 mg/dL and 1.0%, respectively. The successful results represent an important step in the development of a noninvasive, closed-loop, system for monitoring analytes in cell culture.

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