The effect of rosiglitazone in the prevention of intra-abdominal adhesion formation in a rat uterine horn model

Background. Postoperative abdominal adhesion remains one of the frequent complications after abdominal surgery and lacks effective intervention. Peritoneal mesothelial cell injury and healing play crucial roles in the process of adhesion formation, and identifying this mechanism might provide new insight into possible new therapeutic strategies for this disease. Transmembrane and immunoglobulin domain-containing 1 (TMIGD1) has been proven to protect renal epithelial cells from injury induced by oxidative stress and has also been identified as a novel adhesion molecule. Here, we investigated the role of TMIGD1 and its possible mechanism in adhesion formation. Materials and Methods. Immunohistochemistry (IHC), qPCR, and immunofluorescence (IHF) were used to detect the expression of TMIGD1. The grade and tenacity score of adhesion were used to evaluate the adhesion formation conditions. A TMIGD1-overexpressing HMrSV5 cell line was established. MTT assay, Western blotting, Annexin V apoptosis analysis, and CK19 staining were used to measure mesothelial cell viability, apoptosis, and completeness. ROS and MDA detection were used to measure mesothelial cell oxidative stress levels. JC-1 staining, IHF, and transmission electron microscopy were performed to assess mitochondrial function. Scratch-wound and adhesion assays were used to evaluate the adhesion ability of mesothelial cells. Results. First, we showed that TMIGD1 was decreased in mouse abdominal adhesion tissue and peritoneal mesothelial cells. Second, TMIGD1 overexpression inhibited adhesion formation. Third, TMIGD1 overexpression protected mesothelial cells from hydrogen peroxide- (H2O2-) induced oxidative stress injury. Fourth, TMIGD1 overexpression alleviated oxidative stress by protecting the mitochondrial function of mesothelial cells. In addition, TMIGD1 overexpression enhanced mesothelial cell adhesion. Conclusion. Our findings suggest that TMIGD1 protects mesothelial cells from oxidative stress injury by protecting their mitochondrial function, which is decreased in regular abdominal adhesion tissue. In addition, TMIGD1 enhances peritoneal mesothelial cell adhesion to promote healing.

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Synergistic effects of INTERCEED(TC7)* and heparin in reducing adhesion formation in the rabbit uterine horn model*Johnson and Johnson Medical, Inc., New Brunswick, New Jersey.††Presented in part at the 43rd Annual Meeting of The American Fertility Society, Reno, Nevada, September 28 to 30, 1987; and at the 73rd Annual Meeting of The American College of Surgeons, San Francisco, California, October 11 to 16, 1987.‡‡Supported by Johnson and Johnson Medical, Inc., New Brunswick, New Jersey.

Fertility and Sterility ◽

10.1016/s0015-0282(16)54134-x ◽

1991 ◽

Vol 55 (2) ◽

pp. 389-394 ◽

Cited By ~ 86

Author(s):

Michael P. Diamond ◽

Cary B. Linsky ◽

Tim Cunningham ◽

Lola Kamp ◽

Eli Pines ◽

...

Keyword(s):

New Jersey ◽

New Brunswick ◽

Annual Meeting ◽

San Francisco ◽

Synergistic Effects ◽

Adhesion Formation ◽

Uterine Horn ◽

American College Of Surgeons ◽

American Fertility Society ◽

73Rd Annual Meeting

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A Trial of Reducing Adhesion Formation in a Uterine Horn Model

Gynecologic and Obstetric Investigation ◽

10.1159/000009912 ◽

1998 ◽

Vol 45 (1) ◽

pp. 58-61 ◽

Cited By ~ 1

Author(s):

Birol Vural ◽

Ramazan Mercan ◽

Aydın Çorakçı ◽

Semih Özeren ◽

Nadi Keskin ◽

...

Keyword(s):

Adhesion Formation ◽

Uterine Horn

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Systematic review/Meta-analysis A systemic review of randomized controlled studies about prevention with pharmacologic agents of adhesion formation in the rat uterine horn model

Archives of Medical Science ◽

10.5114/aoms.2014.47875 ◽

2015 ◽

Vol 2 ◽

pp. 274-281 ◽

Cited By ~ 4

Author(s):

Gokalp Oner ◽

Pasa Ulug

Keyword(s):

Systematic Review ◽

Meta Analysis ◽

Adhesion Formation ◽

Uterine Horn ◽

Systemic Review ◽

Randomized Controlled ◽

Randomized Controlled Studies ◽

Pharmacologic Agents

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The comparison of methylene blue and vitamin E in prevention of abdominal postoperative adhesion formation in rat uterine horn models: Biochemical and histopathologic evaluation

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000100010 ◽

2011 ◽

Vol 26 (1) ◽

pp. 51-57 ◽

Cited By ~ 3

Author(s):

Hamit Yildiz ◽

Ali Said Durmus ◽

Halil Simsek ◽

Ihsan Yaman

Keyword(s):

Vitamin E ◽

Saline Solution ◽

Adhesion Formation ◽

Uterine Horn ◽

Postoperative Adhesion ◽

Mononuclear Cell Infiltration ◽

The Third ◽

Methylen Blue ◽

Histopathologic Evaluation ◽

Adhesion Model

Purpose: To compare the effects of vitamin E and 1% methylen blue solutions on prevention of experimentally induced adhesions in rats. Methods: Thirty seven female Spraque Dawley rats were randomized into four groups. First group was kept as sham operated group. An adhesion model was constituted on the left uterine horn of the other groups. The lesion areas of rats from the second, the third and the fourth groups were coated with 2 ml 0.9 % saline solution (C group), 10 mg vitamin E (VE group) and 1% methylen blue solutions (MB group), respectively. Results: Histopathologically, adhesion scores, mononuclear cell infiltration, oedema and fibrosis were more prominent in the MB group compared with C and VE groups. There were no significant differences between the groups in tissue glutathione peroxidase (GPx), catalase (CAT) activities and glutation (GSH) level, these parameters were slightly increased in group with VE supplementation though. The administration of VE and MB significantly decreased NO (P<0.01) levels when compared to the C group. The level of malondialdehyde (MDA) in the VE group was significantly lower (P<0.05) than those of the Sh and C groups. Conclusion: Intraperitoneal methylen blue solutions treatments were more effective according to vitamin E in preventing the formation of intra-abdominal adhesion in a rat uterine horn model.

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