Enterotoxigenic Escherichia coli Carrying Plasmids Coding for Antibiotic Resistance and Enterotoxin Production

1980 ◽  
Vol 142 (2) ◽  
pp. 273-278 ◽  
Author(s):  
P. Echeverria ◽  
J. R. Murphy
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Enterotoxigenic Escherichia Coli ◽  
Enterotoxin Production

Role of enterotoxigenic Escherichia coli prophage in spreading antibiotic resistance in a porcine‐derived environment

2020 ◽  
Vol 22 (12) ◽  
pp. 4974-4984 ◽  
Author(s):  
Mianzhi Wang ◽  
Zhenling Zeng ◽  
Fengwei Jiang ◽  
Ying Zheng ◽  
Huigang Shen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Enterotoxigenic Escherichia Coli

Further characterization of Escherichia coli O153:H45, an ETEC serotype disseminated in Chile

1988 ◽  
Vol 34 (1) ◽  
pp. 85-88 ◽  
Author(s):  
M. Elena Fernández-Beroš ◽  
Vincent Kissel ◽  
Maria E. Agüero ◽  
Guillermo Figueroa ◽  
Karen D'Ottone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Enterotoxigenic Escherichia Coli ◽  
Plasmid Content ◽  
Enterotoxin Genes ◽  
Metabolic Properties ◽  
Factor Antigen

The newly described stable enterotoxin producing, enterotoxigenic Escherichia coli, serotype O153:H45, capable of expressing colonizing factor antigen I, is frequently isolated as a cause of diarrhea among Chilean children. Hybridization studies of five new strains confirmed previous results which indicated that the stable enterotoxin genes are contained in nonconjugative plasmids ranging in size from 81 to 87 kilobases. The strains expressed similar antibiotic resistance and metabolic properties but differed in their plasmid content.

scholarly journals Comparison of virulence markers and antibiotic resistance in enterotoxigenic Escherichia coli isolated ten years apart in Tehran

2010 ◽  
Vol 5 (04) ◽  
pp. 248-254 ◽  
Author(s):  
Nader Shahrokhi ◽  
Saeid Bouzari ◽  
Anis Jafari
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Dna Hybridization ◽  
Pathogenic Bacteria ◽  
Enterotoxigenic Escherichia Coli ◽  
Significant Shift ◽  
Bacterial Populations ◽  
Virulence Markers ◽  
Class 1 ◽  
Resistance Patterns

Introduction: Enterotoxigenic Escherichia coli (ETEC) causes diarrhoea by producing heat-labile (LT) or heat-stable (ST) enterotoxins after colonizing the small intestine by means of colonization factors (CFs). Although detection of the toxins is sufficient for verification of ETEC isolates, toxin-positive strains may be further analyzed for the presence of CFs. Antibiotics may shorten the duration of diarrhoea caused by ETEC, but the rapid emergence of resistant strains limits their usefulness. Methodology: ETEC isolates collected 10 years apart were compared for the prevalence of toxin types, CFs and antibiotic resistance. DNA/DNA hybridization with digoxigenin (DIG)-labeled probes was used for the detection of toxin types, and CF-typing was performed by DNA hybridization using DIG-labeled probes for cfaD and CS6 with slide agglutination. Disk diffusion was used to determine antibiotic resistance. The presence of class 1 integrons was detected by PCR. Results: ST-positive isolates were the most prevalent among the isolates from 1988, but a significant shift towards LT-gene carriage was observed in the 1998 group. CFA/I and CFA/IV were the most common CF types within both groups. The most prevalent resistance patterns among these isolates were ACSTSXT followed by ASTSXT and ASSXT. Conclusion: Our study of the two groups of isolates showed that the rate of LT and ST gene carriage, as well as antibiotic resistance markers, has changed in the ten years separating the two bacterial populations. These variations show the importance of monitoring pathogenic bacteria to obtain a near realistic picture of the circulating bacterial pathogens.

scholarly journals Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli.

1979 ◽  
Vol 23 (2) ◽  
pp. 325-329 ◽  
Author(s):  
M H Merson ◽  
F Orskov ◽  
I Orskov ◽  
R B Sack ◽  
I Huq ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Enterotoxin Production

scholarly journals Prevalence of enteropathogenic and enterotoxigenic Escherichia Coli in foods of animal origin in southern Brazil

1996 ◽  
Vol 26 (2) ◽  
pp. 247-250 ◽  
Author(s):  
José Antonio Aleixo ◽  
Gladis Paim Aver
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Food Samples ◽  
Enterotoxigenic Escherichia Coli ◽  
Animal Origin ◽  
Resistance Pattern ◽  
Southern Brazil ◽  
E Coli ◽  
Highly Sensitive ◽  
The City

Prevalence of Escherichia coli in foods of animal origin from the city of Pelotas, Brazil, was determined. The ocurrence of enterotoxigenic (ETEC) and classic enteropathogenic (EPEC) serogroups among E. coli isolates was determined. E. coli was isolated from 68% of the food samples surveyed. Of 36 food samples tested, 11(30%) and 24(66%) were positive for EPEC and ETEC strains respectively. However, of 187 E. coli isolates tested, 30(16%) were EPEC compared to 75(40%) which were ETEC. The antibiotic resistance pattern revealed that the isolates were highly sensitive to all antibiotics tested.

scholarly journals Antimicrobial Resistance Genes in Enterotoxigenic Escherichia coli O149:K91 Isolates Obtained over a 23-Year Period from Pigs

2003 ◽  
Vol 47 (10) ◽  
pp. 3214-3221 ◽  
Author(s):  
Christine Maynard ◽  
John M. Fairbrother ◽  
Sadjia Bekal ◽  
François Sanschagrin ◽  
Roger C. Levesque ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Antimicrobial Agents ◽  
Enterotoxigenic Escherichia Coli ◽  
Genotypic Resistance ◽  
Class 1 Integron ◽  
Phenotypic Resistance ◽  
Antimicrobial Resistance Genes

ABSTRACT A total of 112 Escherichia coli O149:K91 strains isolated from pigs with diarrhea in Quebec, Canada, between 1978 and 2000 were characterized for their genotypic antimicrobial resistance profiles. Tests for resistance to 10 antimicrobial agents were conducted. Resistance to tetracycline and sulfonamides was found to be the most frequent, but resistance to cefotaxime and ceftiofur was absent. An increase in the number of isolates resistant to at least three antimicrobials was observed over time. The distribution of 28 resistance genes covering six antimicrobial families (beta-lactams, aminoglycosides, phenicols, tetracycline, trimethoprim, and sulfonamides) was assessed by colony hybridization. Significant differences in the distributions of tetracycline [tet(A), tet(B), tet(C)], trimethoprim (dhfrI, dhfrV, dhfrXIII), and sulfonamide (sulI, sulII) resistance genes were observed during the study period (1978 to 2000). Sixty percent of the isolates possessed a class 1 integron, illustrating the importance of integrons in the epidemiology of antibiotic resistance in E. coli strains from pigs. Amplification of the integron's variable region resulted in four distinct fragments of 1, 1.3, 1.6, and 1.8 kb, with the 1.6- and 1.8-kb fragments appearing only during the last half of the study period. Examination of linkages among the different resistance genes showed a variety of positive and negative associations. Association analysis of isolates divided into two groups, those isolated between 1978 and 1989 and those isolated between 1990 and 2000, revealed the appearance of new positive resistance gene associations. Our genotypic resistance analyses of ETEC isolates from pigs indicate that many of the antibiotic resistance genes behind phenotypic resistance are not static but, rather, are in a state of flux driven by various selection forces such as the use of specific antimicrobials.

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