Cryptosporidium parvum Infection of Intestinal Epithelium: Morphologic and Functional Studies in an In Vitro Model

1994 ◽  
Vol 169 (1) ◽  
pp. 170-177 ◽  
Author(s):  
R. B. Adams ◽  
R. L. Guerrant ◽  
S. Zu ◽  
G. Fang ◽  
J. K. Roche
Keyword(s):  
Cryptosporidium Parvum ◽  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Functional Studies ◽  
Vitro Model

Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium

2010 ◽  
Vol 188 (3) ◽  
pp. 659-667 ◽  
Author(s):  
Thérèse Sergent ◽  
Neil Piront ◽  
Julie Meurice ◽  
Olivier Toussaint ◽  
Yves-Jacques Schneider
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Anti Inflammatory ◽  
Vitro Model ◽  
Human Intestinal Epithelium

The Perfused Human Liver Wedge Biopsy: A New In Vitro Model for Morphological and Functional Studies

Hepatology ◽  
2007 ◽  
Vol 2 (4) ◽  
pp. 426S-432S ◽  
Author(s):  
Susan J. Burwen ◽  
Albert L. Jones ◽  
Ira S. Goldman ◽  
Lawrence W. Way ◽  
Sussan Dejbakhsh
Keyword(s):  
Human Liver ◽  
In Vitro Model ◽  
Functional Studies ◽  
Vitro Model ◽  
Wedge Biopsy

scholarly journals Requirement for CD40-CD40 Ligand Interaction for Elimination of Cryptosporidium parvum from Mice

1998 ◽  
Vol 66 (2) ◽  
pp. 603-607 ◽  
Author(s):  
Mary Cosyns ◽  
Svetlana Tsirkin ◽  
Michelle Jones ◽  
Richard Flavell ◽  
Hitoshi Kikutani ◽  
...  
Keyword(s):  
Cryptosporidium Parvum ◽  
In Vitro Model ◽  
Biliary Tree ◽  
Cd40 Ligand ◽  
Ligand Interaction ◽  
Cell Responses ◽  
Infected Cells ◽  
Vitro Model

ABSTRACT Mice with disrupted genes for CD40 and CD40 ligand (CD40L) are unable to clear infection with Cryptosporidium parvum and develop cholangitis. Parasites are present in the gut, gall bladder, and biliary tree, and biliary epithelial cells express CD40 on the cell surface. SCID mice infected with C. parvum for >1 month can clear the infection after reconstitution with spleen cells from CD40, but not CD40L, knockout mice. In an in vitro model, C. parvum-infected HepG2 cells were triggered to apoptosis when incubated with a CD40L-CD8 fusion protein. The requirement for CD40-CD40L interactions for immunity to C. parvum indicated by our results may entail the triggering of apoptosis in infected cells, in addition to the known role of CD40L-CD40 interactions in stimulating cytokine production and promoting T-cell responses.

scholarly journals Characterizing Phage-Host Interactions in a Simplified Human Intestinal Barrier Model

2020 ◽  
Vol 8 (9) ◽  
pp. 1374
Author(s):  
María A. Núñez-Sánchez ◽  
Joan Colom ◽  
Lauren Walsh ◽  
Colin Buttimer ◽  
Andrei Sorin Bolocan ◽  
...  
Keyword(s):  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Cell Model ◽  
Layer Structure ◽  
Intestinal Barrier ◽  
Host Interactions ◽  
Intestinal Epithelia ◽  
Vitro Model ◽  
Barrier Model

An intestinal epithelium model able to produce mucus was developed to provide an environment suitable for testing the therapeutic activity of gut bacteriophages. We show that Enterococcus faecalis adheres more effectively in the presence of mucus, can invade the intestinal epithelia and is able to translocate after damaging tight junctions. Furthermore, Enterococcus phage vB_EfaM_A2 (a member of Herelleviridae that possesses virion associated immunoglobin domains) was found to translocate through the epithelium in the presence and absence of its host bacteria. Phage A2 protected eukaryotic cells by reducing mortality and maintaining the structure of the cell layer structure. We suggest the mammalian cell model utilized within this study as an adaptable in vitro model that can be employed to enable a better understanding of phage–bacteria interactions and the protective impact of phage therapy relating to the intestinal epithelium.

Toxicity, uptake, and nuclear translocation of ingested micro-nanoplastics in an in vitro model of the small intestinal epithelium

2021 ◽  
pp. 112609
Author(s):  
Glen M. DeLoid ◽  
Xiaoqiong Cao ◽  
Dimitrios Bitounis ◽  
Dilpreet Singh ◽  
Paula Montero Llopis ◽  
...  
Keyword(s):  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Nuclear Translocation ◽  
Small Intestinal Epithelium ◽  
Small Intestinal ◽  
Vitro Model

scholarly journals The Effects of Nitrogen-Containing Bisphosphonates on Human Epithelial (Caco-2) Cells, an In Vitro Model for Intestinal Epithelium

1999 ◽  
Vol 14 (5) ◽  
pp. 784-791 ◽  
Author(s):  
I. M. Twiss ◽  
O. Pas ◽  
W. Ramp-Koopmanschap ◽  
J. Den Hartigh ◽  
P. Vermeij
Keyword(s):  
Intestinal Epithelium ◽  
In Vitro Model ◽  
Vitro Model
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