Note on Paper Chromatographic Detection of Adulteration in Dark Colored Fruit Juices

1970 ◽  
Vol 53 (3) ◽  
pp. 578-579
Author(s):  
J Fitelson

Abstract A second collaborative study was carried out on the official first action method for dark colored fruit juices. The method is based mainly on differences in the anthocyanin patterns of adulterated fruit juices when compared with authentic samples. Natural colors used for such adulteration show more complex patterns than those of the dark juices, so that such addition can usually be detected. Anthocyanidin patterns yield much less information than the anthocyanin patterns. Eleven collaborators correctly detected adulteration in a blackberry juice with added invert sugar and elderberry juice, and 10 of them correctly found adulteration in a raspberry juice with added apple juice and grape skin extract. The method is recommended for adoption as official final action.

1967 ◽  
Vol 50 (2) ◽  
pp. 293-299 ◽  
Author(s):  
J Fitelson

Abstract Paper chromatography of the anthocyanidins, the phenolic components of the anthocyanins, is the most satisfactory means of detecting adulteration of Concord grape juice with other red grape juice and colored Italian grape skin extract. The 5 aniliocyanidins found in grape colors can be readily separated into 3 spots by paper chromatography. In the proposed method, anthocyanins are separated by lead precipitation, dissolved in water, and acid-hydrolyzed to obtain the anthocyanidins. Thirty Concord samples, 7 red grape juice samples, and 5 Italian grape skin extracts were examined and results were good. Four unknown samples were analyzed by 5 collaborators; all collaborators correctly detected the adulterated sample and the pure Concord sample. This method was recommended for adoption as official, first action


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