Simple Colorimetric Method for Determination of Pyridoxine Hydrochloride (Vitamin B6) in Pharmaceuticals

1983 ◽  
Vol 66 (1) ◽  
pp. 158-160
Author(s):  
Ramesh T Sane ◽  
Vipul J Doshi ◽  
Sanjay K Joshi
Keyword(s):  
Aqueous Solution ◽  
Sulfuric Acid ◽  
Vitamin B6 ◽  
Trichloroacetic Acid ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Thiamine Hydrochloride ◽  
Pyridoxine Hydrochloride ◽  
Statistical Validation

Abstract A simple colorimetric method is described for the determination of pyridoxine hydrochloride (vitamin B6). The method is based on the measurement of an orange species formed when pyridoxine hydrochloride is treated with diazotized dapsone and sulfanilamide in a mixture of trichloroacetic acid and sulfuric acid at room temperature, followed by treatment with an aqueous solution of sodium carbonate. Compounds such as thiamine hydrochloride, cyanocobalamin, and common excipients such as starch and talc which are present in various formulations with pyridoxine hydrochloride do not interfere in the reaction. Statistical validation showed that the method was highly precise and accurate. Results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination of Metaproterenol Sulfate in Pharmaceuticals

1984 ◽  
Vol 67 (1) ◽  
pp. 137-138
Author(s):  
Ramesh T Sane ◽  
Satish V Sawant ◽  
Vipul J Doshi ◽  
Jagannath G Mhalas ◽  
Ajay K Paarikh ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Statistical Validation

Abstract A simple coiorimetric method is described for the determination of metaproterenol sulfate (orciprenaline sulfate). The method is based on measurement of a colored species formed when metaproterenol sulfate is treated with diazotized dapsone, p-nitroaniline, or benzocaine at room temperature, followed by treatment with an aqueous solution of trimethylamine in the case of benzocaine. Compounds such as starch, talc, and common excipients do not interfere in the reaction. Statistical validation showed that the method was precise and accurate. The results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination from Pharmaceutical Preparations

1986 ◽  
Vol 69 (2) ◽  
pp. 186-188 ◽  
Author(s):  
Ramesh T Sane ◽  
Vishwanath B Malkar ◽  
Vinay G Nayak ◽  
Dhananjay S Sapre
Keyword(s):  
Room Temperature ◽  
Colorimetric Method ◽  
Pharmaceutical Preparations ◽  
Statistical Validation ◽  
Terbutaline Sulfate

Abstract A simple colorimetric method is described for the determination of terbutaline sulfate. The method is based on measurement of a colored species formed when terbutaline sulfate is treated with diazotized dapsone and p-nitroaniline at room temperature. Compounds such as starch, talc, and common excipients do not interfere in the reaction. Statistical validation showed that the method was highly precise and accurate. The results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination of Thiamine Hydrochloride in Pharmauceuticals

1985 ◽  
Vol 68 (1) ◽  
pp. 83-85
Author(s):  
Ramesh T Sane ◽  
Vipul J Doshi ◽  
Swati Jukar ◽  
Sanjay K Joshi ◽  
Satish V Sawant ◽  
...  
Keyword(s):  
Liver Extract ◽  
Colorimetric Method ◽  
Dosage Forms ◽  
Vitamin B ◽  
Thiamine Hydrochloride ◽  
Calcium Pantothenate ◽  
Statistical Validation ◽  
Alkaline Conditions ◽  
The Stability

Abstract A simple colorimetric method is described for the determination of thiamine hydrochloride (vitamin B,) in dosage forms. The method is based on measurement of a yellow complex formed when thiamine HC1 is treated with /7-methylaminophenol sulfate (Metol) under alkaline conditions. Compounds such as vitamins A, B2, B6, B,2, C, D, and E, and niacinamide, citric acid, liquid glucose, calcium pantothenate, biotin, liver extract, and folic acid do not interfere in the reaction. Extracting the complex into chloroform before quantitation enhances the stability of the reaction product and removes interference of watersoluble colored constituents in syrup samples. Statistical validation shows that the method is precise and accurate. Results agree well with those obtained by other methods in the literature

scholarly journals DETERMINATION OF THIAMINE HCL (VITAMIN B1) AND PYRIDOXINE HCL (VITAMIN B6) CONTENT IN TABLET BY FTIR

2016 ◽  
Vol 8 (10) ◽  
pp. 257 ◽  
Author(s):  
Ilma Nugrahani ◽  
Citra Kartini
Keyword(s):  
Vitamin B6 ◽  
Dosage Form ◽  
Vitamin B1 ◽  
Area Under The Curve ◽  
Thiamine Hydrochloride ◽  
Compound Identification ◽  
Quantitation Limit ◽  
Validation Parameters ◽  
Tablet Dosage

<p><strong>Objective: </strong>The combination of thiamine hydrochloride (vitamin B1) with pyridoxine hydrochloride (vitamin B6) has been efficacious to help the metabolism of carbohydrates and amino acids. FTIR (Fourier transform infrared) is a technique widely used in compound identification and determination of functional groups but rarely used for the quantitative purposes. This study aims to obtain a analysis determination method of this vitamin combination simultaneously in tablet dosage form using FTIR.</p><p><strong>Methods: </strong>Amount of vitamin B1 and B6 standard were mixed with KBr crystal in a series of concentration (% w/w) in kalium bromide (kbr) crystal, then measured with FTIR. These spectrums yielded were transformed into an absorbance afterward changed to its derivative. The finest spectrum, which showed the best specificity and linearity, was selected and its area under the curve was calculated. The other validation parameters: accuracy, precision, detection limit, quantitation limit, and ranges, next were tested and determined. The validated method than was used to analyze the levels of vitamin B1 and B6 simultaneously in the tablets.</p><p><strong>Results: </strong>Vitamin B1 and B6 have the linear concentration range from 0.5 to 3.00% w/w. The regression equation for vitamin B1 is y = 0.0608 x-0.0176 with r = 0.9997 and Vx0 = 1.5047%, for vitamin B6: y = 0.0977 x+0.0079 r = 0.9995 and Vx0 = 1.7832%. Recovery results of vitamin B1: 98.98 to 100.93%, while B6: 99.06 to 100.43%. Intra-day and inter-day precision for vitamin B1: 1.73; 1.62; 1.48, and 0.58%, meanwhile for vitamin B6: 1.29; 1.60; 1.78, and 1.39%. The limits of detection and quantitation for vitamin B12 was 0.079 and 0.263% w/w respectively, and for vitamin B6, was: 0.093 and 0.311% w/w. The tablets from the market were tested showed the results that meet with compendia requirements.</p><p><strong>Conclusion: </strong>FTIR can be used for the determination of levels of vitamin B1 and B6 simultaneously in tablet dosage form and have met the validation requirements.</p>

Collaborative Study Using a ZDBT Colorimetric Method for the Determination of Copper in Alcoholic Products

1967 ◽  
Vol 50 (2) ◽  
pp. 334-338
Author(s):  
Duane H Strunk ◽  
A A Andreasen
Keyword(s):  
Sulfuric Acid ◽  
Carbon Tetrachloride ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Good Precision ◽  
Determination Of Copper

Abstract Results are given on a collaborative study in which a zinc dibenzyldithiocarbamate (ZDBT) colorimetric method is used to measure copper in alcoholic products such as high wine, spirits, gin, whisky, brandy, rum, and wine. In this method, the sample is made ca 0.SN with sulfuric acid, and carbon tetrachloride containing 0.2% ZDBT is added. The colored copper-ZDBT complex is extracted in the carbon tetrachloride and measured at 438 mμ against a similar carbon tetrachloride extract of a blank. Data show good precision, and it is recommended that the ZDBT method be adopted as official, first action.

Collaborative Study of a Method for Sodium Lauryl Sulfate in Egg Whites

1968 ◽  
Vol 51 (3) ◽  
pp. 540-543
Author(s):  
John Wiskerchen
Keyword(s):  
Sulfuric Acid ◽  
Quantitative Determination ◽  
Sodium Lauryl Sulfate ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Egg White ◽  
Mean Deviation ◽  
Lauryl Sulfate ◽  
Benzethonium Chloride

Abstract A colorimetric method for the quantitative determination of sodium lauryl sulfate in liquid, frozen, and powdered egg white was studied by eight collaborators. Determinations were made on flake and powdered egg white at levels of 0.05, 0.1, and 0.2% (w/w) and on liquid egg white at levels of 0.006, 0.0125, and 0.0250% (w/w) sodium lauryl sulfate. The egg white is dissolved in water, and the protein is precipitated with ethanol and removed by filtration. An aliquot of the filtrate is evaporated to dryness, and the residue is dissolved in water and acidified with sulfuric acid. The sodium lauryl sulfate is complexed with Azure A, extracted into chloroform, and determined spectrophotometrically at 637 mμ. A blank determination is made on another aliquot of the filtrate by complexing the sodium lauryl sulfate with benzethonium chloride. This is a stable colorless complex. Average recoveries in the collaborative study were 98—102% with a mean deviation of 2.8—5.4%. It is recommended that the method be adopted as official, first action.

Colorimetric Determination of Dillapiole in Wheat

1979 ◽  
Vol 62 (1) ◽  
pp. 203-204
Author(s):  
Swadesh K Handa
Keyword(s):  
Sulfuric Acid ◽  
Colorimetric Method ◽  
Chromotropic Acid ◽  
Colorimetric Determination ◽  
Wheat Grain ◽  
Maximum Absorbance

Abstract A rapid colorimetric method has been developed for determining residues of dillapiole (3,4-methylenedioxy-5,6-dimethoxy allyl benzene) in wheat grain. Dillapiole is reacted with sulfuric acid to liberate formaldehyde which reacts with chromotropic acid to yield a pink compound with maximum absorbance at 575 nm. The method can detect 1—15 μg residue /ml.

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