Simple Colorimetric Method for Determination of Metaproterenol Sulfate in Pharmaceuticals

1984 ◽  
Vol 67 (1) ◽  
pp. 137-138
Author(s):  
Ramesh T Sane ◽  
Satish V Sawant ◽  
Vipul J Doshi ◽  
Jagannath G Mhalas ◽  
Ajay K Paarikh ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Statistical Validation

Abstract A simple coiorimetric method is described for the determination of metaproterenol sulfate (orciprenaline sulfate). The method is based on measurement of a colored species formed when metaproterenol sulfate is treated with diazotized dapsone, p-nitroaniline, or benzocaine at room temperature, followed by treatment with an aqueous solution of trimethylamine in the case of benzocaine. Compounds such as starch, talc, and common excipients do not interfere in the reaction. Statistical validation showed that the method was precise and accurate. The results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination of Pyridoxine Hydrochloride (Vitamin B6) in Pharmaceuticals

1983 ◽  
Vol 66 (1) ◽  
pp. 158-160
Author(s):  
Ramesh T Sane ◽  
Vipul J Doshi ◽  
Sanjay K Joshi
Keyword(s):  
Aqueous Solution ◽  
Sulfuric Acid ◽  
Vitamin B6 ◽  
Trichloroacetic Acid ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Thiamine Hydrochloride ◽  
Pyridoxine Hydrochloride ◽  
Statistical Validation

Abstract A simple colorimetric method is described for the determination of pyridoxine hydrochloride (vitamin B6). The method is based on the measurement of an orange species formed when pyridoxine hydrochloride is treated with diazotized dapsone and sulfanilamide in a mixture of trichloroacetic acid and sulfuric acid at room temperature, followed by treatment with an aqueous solution of sodium carbonate. Compounds such as thiamine hydrochloride, cyanocobalamin, and common excipients such as starch and talc which are present in various formulations with pyridoxine hydrochloride do not interfere in the reaction. Statistical validation showed that the method was highly precise and accurate. Results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination from Pharmaceutical Preparations

1986 ◽  
Vol 69 (2) ◽  
pp. 186-188 ◽  
Author(s):  
Ramesh T Sane ◽  
Vishwanath B Malkar ◽  
Vinay G Nayak ◽  
Dhananjay S Sapre
Keyword(s):  
Room Temperature ◽  
Colorimetric Method ◽  
Pharmaceutical Preparations ◽  
Statistical Validation ◽  
Terbutaline Sulfate

Abstract A simple colorimetric method is described for the determination of terbutaline sulfate. The method is based on measurement of a colored species formed when terbutaline sulfate is treated with diazotized dapsone and p-nitroaniline at room temperature. Compounds such as starch, talc, and common excipients do not interfere in the reaction. Statistical validation showed that the method was highly precise and accurate. The results agree well with those obtained by other methods reported in the literature.

Simple Colorimetric Method for Determination of Thiamine Hydrochloride in Pharmauceuticals

1985 ◽  
Vol 68 (1) ◽  
pp. 83-85
Author(s):  
Ramesh T Sane ◽  
Vipul J Doshi ◽  
Swati Jukar ◽  
Sanjay K Joshi ◽  
Satish V Sawant ◽  
...  
Keyword(s):  
Liver Extract ◽  
Colorimetric Method ◽  
Dosage Forms ◽  
Vitamin B ◽  
Thiamine Hydrochloride ◽  
Calcium Pantothenate ◽  
Statistical Validation ◽  
Alkaline Conditions ◽  
The Stability

Abstract A simple colorimetric method is described for the determination of thiamine hydrochloride (vitamin B,) in dosage forms. The method is based on measurement of a yellow complex formed when thiamine HC1 is treated with /7-methylaminophenol sulfate (Metol) under alkaline conditions. Compounds such as vitamins A, B2, B6, B,2, C, D, and E, and niacinamide, citric acid, liquid glucose, calcium pantothenate, biotin, liver extract, and folic acid do not interfere in the reaction. Extracting the complex into chloroform before quantitation enhances the stability of the reaction product and removes interference of watersoluble colored constituents in syrup samples. Statistical validation shows that the method is precise and accurate. Results agree well with those obtained by other methods in the literature

New colorimetric method for quantitative determination of protein in urine.

1977 ◽  
Vol 23 (5) ◽  
pp. 811-812 ◽  
Author(s):  
H Yatzidis
Keyword(s):  
Quantitative Determination ◽  
Tannic Acid ◽  
Chloride Solution ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Ferric Chloride Solution ◽  
Acid Protein ◽  
Analytical Recovery ◽  
Violet Color

Abstract Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected. The CV and analytical recovery ranged from 0.5 to 1.8% and 98 to 103%, respectively. Nonprotein urinary constituents do not interfere.

Rapid Colorimetric Method for the Determination of Methyl Salicylate in Medicated Oils

1966 ◽  
Vol 49 (4) ◽  
pp. 854-856
Author(s):  
M C Dutt
Keyword(s):  
Aqueous Solution ◽  
Ferric Chloride ◽  
Calibration Curve ◽  
Methyl Salicylate ◽  
Quantitative Estimation ◽  
Colorimetric Method ◽  
Synthetic Mixture ◽  
Excess Water ◽  
Ether Solution

Abstract The well-known reaction between methyl salicylate and ferric chloride has been successfully applied to the quantitative estimation of methyl salicylate in medicated oils. An aqueous solution of ferric chloride is added to an ether solution of medicated oil dissolved in excess water. After filtering, the absorbance of the filtrate is measured at 530 m/j, and the content of methyl salicylate is determined from a calibration curve. Methyl salicylate recoveries range from 92 to 97% for concentrations between 10 and 60% in a synthetic mixture. The method is simple and rapid, and compares favorably with Wilson’s method.

Darstellung, Schwingungsspektren und Normalkoordinatenanalysen der Dioxoosmate(VI) trans-[OsO2(CN)2(ox)]2-, transs-[OsO2(CN)2(mal)]2- und trans-[OsO2(CN)2(N2H2C2O2)]2- / Synthesis, Vibrational Spectra and Normal Coordinate Analysis of the Dioxoosmates(VI) frans-[OsO2(CN)2(ox)]2- , trans-[OsO2 (CN)2(mal)]2- and trans-[OsO2 (CN)2(N2H2C2O2)]2-

1999 ◽  
Vol 54 (9) ◽  
pp. 1116-1121
Author(s):  
A. Strueß ◽  
W. Preetz
Keyword(s):  
Aqueous Solution ◽  
Oxalic Acid ◽  
Raman Spectra ◽  
Malonic Acid ◽  
Vibrational Spectra ◽  
Room Temperature ◽  
Normal Coordinate ◽  
X Ray ◽  
Ir And Raman

By careful acidification of the aqueous solution of trans-K2[OsO2(OH)4] in the presence of the required amount of cyanide ions with oxalic acid, malonic acid or oxamide the osmyl complexes trans-[OsO2(CN)2(ox)]2- (1), trans-[OsO2(CN)2(mal)]2- (2) und trans-[OsO2(CN)2(N2H2C2O2)]2- (3) are formed. The IR and Raman spectra of the (n-Bu4N) and (Et4N) salts of 1, 2 und 3 were measured at room temperature. Based on the molecular parameters of the X-ray determination of related complexes normal coordinate analyses have been performed and the vibrations were assigned. The valence force constants are fd(C≡N ) = 16.95, fd(Os=O) = 6.68 - 6.70, fd(Os-O) = 2.55 - 2.60, fd(Os-C) = 2.55 and fd(Os-N) = 2.30 mdyn/Å. For the chelate ligands, fd(C =0) ranges from 11.03 - 11.15, fd(C-O/N) from 4.86 - 5.05 and fd(C-C) from 4.07 - 4.70 mdyn/Å.

scholarly journals Determination of starch by iodine colorimetry

1968 ◽  
Vol 40 (2) ◽  
pp. 60-66
Author(s):  
K. A. Vainio
Keyword(s):  
Room Temperature ◽  
Potato Starch ◽  
Starch Content ◽  
Colorimetric Method ◽  
Iodine Concentration ◽  
Wheat Starch ◽  
Laboratory Animals ◽  
Concentration Limit ◽  
Minimum Concentration

In the iodine colorimetric method of Paloheimo gently dextrinized solutions are prepared of pure starch and of the analysis sample. One of the optical cells (A) of the comparator is provided with a solution made of pure starch and the other (B) with the solution to be analysed. Both solutions have the same iodine concentration. The solution in B must have a intensive colour than that in A. Solution B is then diluted with an iodinewater solution of the same iodine concentration as in the solutions A and B. When these solutions have attained the same colour it is concluded that also the starch concentration is the same and the starch content of the sample can be calculated. The results obtained by this method are compared with those obtained with the amyloglucosidase method of Salo. Table 1 shows that the two methods give very similar results. Different circumstances which might possibly interfere with the colorimetric starch determinations are studied. It was observed that attention must be paid to the intensity of boiling when the 0.05-N H2SO4 dextrinizing solutions are boiled. If the intensity is very different in the comparison solution and the solution to be analysed, considerable errors may occur. If the sample contains added chalk the neutralizing power of the sample should be determined beforehand and the normality of the solution adjusted to 0.05. If the sample contains acid it should be extracted beforehand with 80-% ethanol. —Cellulose and sugars have no influence on the results, nor have plant proteins or proteins of milk. However, if greater amounts of protein were added, a casein preparation intended for laboratory animals showed an obvious disturbing effect, as did gelatin and meat protein. – Faeces did not appear to have an interfering influence in colorimetric starch determination. The iodine colorimetric sensitiveness of starch solutions was also studied. It appeared that 0.18 mg of dextrinized potato starch already deepened the colour of 100 ml dilute iodine solution in room temperature. For wheat starch the corresponding minimum concentration was 0.27 mg/100 ml. In 3° the concentration limit was even lower, 0.05—0.09 mg/100 ml. In all the above mentioned studies the author has used as comparator essential parts of a Pulfrich photometer. A proper comparator (Fig. 1) can also be made by any skilled optician.

scholarly journals Determination of the antioxidant activity of Ficus carica aqueous extract

2012 ◽  
pp. 25-31 ◽  
Author(s):  
Svetlana Trifunski ◽  
Dorina Ardelean
Keyword(s):  
Antioxidant Activity ◽  
Traditional Medicine ◽  
Potassium Permanganate ◽  
Aqueous Extract ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Water Extract ◽  
Leaf Water ◽  
Water Extracts

The aim of this study was to examine the antioxidant activity of water extracts from fig leaf. Water extracts were prepared according to traditional medicine. The antioxidant activity of the extracts was spectrophotometrically determined. Using the potassium permanganate colorimetric method it was found that the water extract that was maintained at the refrigerator had lower antioxidant activity than extract that was maintained at the room temperature.

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