Intercomparative Study on the Determination of Polynuclear Aromatic Hydrocarbons in Marine Shellfish Tissue

1988 ◽  
Vol 71 (2) ◽  
pp. 363-368 ◽  
Author(s):  
John F Uthe ◽  
Charles J Musial
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Statistical Analysis ◽  
Aromatic Hydrocarbons ◽  
Polynuclear Aromatic Hydrocarbons ◽  
The Other ◽  
Acetone Powder ◽  
Relative Standard ◽  
Polycyclic Aromatic

Abstract Twenty-five laboratories were sent 2 materials, one an acetone powder of lobster digestive gland, the other, the oil which had been extracted during preparation of the powder. Each laboratory was requested to measure the levels of a suite of polycyclic aromatic hydrocarbons in both materials. The response was poor with only 10 laboratories submitting results. Both intra- and interlaboratory precisions were poor; the interlaboratory error was so great as to preclude statistical analysis of the error. Relative standard deviations for oil results determined by liquid chromatography ranged from 39 to 96%.

Rapid, Semimicro Method for Determination of Polycyclic Aromatic Hydrocarbons in Shellfish by Automated Gel Permeation/Liquid Chromatography

1986 ◽  
Vol 69 (3) ◽  
pp. 462-466 ◽  
Author(s):  
Charles J Musial ◽  
John F Uthe
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
Screening Method ◽  
Homarus Americanus ◽  
Automated Method ◽  
Relative Standard ◽  
Gel Permeation ◽  
Polycyclic Aromatic

Abstract A simple, rapid, easily automated method is described for the determination of polycyclic aromatic hydrocarbons (PAHs) in shellfish such as American lobster (Homarus americanus) and blue mussel (Mytilus edulis). PAHs are extracted from small amounts (1-8 g) of tissue by saponification in IN ethanolic potassium hydroxide followed by partitioning into 2,2,4-trimethylpentane. This solution is evaporated just to dryness by rotary evaporation and the residue is dissolved in cyclohexane- dichloromethane (1 + 1) for gel permeation chromatography (GPC) on Bio-Beads SX-3. The GPC procedure is ideal as a screening method in the range 25-18 000 ng PAHs/g tissue. If individual PAH measurements are required, the appropriate GPC fraction is collected and PAHs are separated by reverse phase liquid chromatography (LC) with fiuorometric detection. Individual PAHs at concentrations as low as 0.25-10 ng/g can be determined. Recoveries of added fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[e]pyrene, benzo[A]fluoranthene, benzo[b]fluoranthene, benzo[α]pyrene, dibenz[a,h]anthracene, benzo[ghi]perylene, and indeno[l,2,3-cd]pyrene were quantitative, with relative standard deviations ranging from 0.0 to 16.9%.

scholarly journals Determination of Polycyclic Aromatic Hydrocarbons from Buzzards (Buteo buteo) and Tawny Owl (Strix aluco) by Liquid Chromatography with Fluorescence Detection

2002 ◽  
Vol 85 (1) ◽  
pp. 141-145 ◽  
Author(s):  
Susana González Amigo ◽  
Maria Asunción Lage Yusty ◽  
Jesús Simal Lozano
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Aromatic Hydrocarbons ◽  
Tawny Owl ◽  
Relative Standard ◽  
Polycyclic Aromatic ◽  
Predatory Birds ◽  
Lung And Kidney

Abstract Supercritical fluid extraction was applied to the determination of naturally contaminated polycyclic aromatic hydrocarbons (PAHs) in bird tissue by liquid chromatography with fluorescence detection (LC-FL). Recoveries (>90%) and relative standard deviations (≤7.7%) were satisfactory. The levels of 10 PAHs were analyzed in 6 classes of tissues (heart, liver, intestine, muscle, lung, and kidney) of 10 buzzards and 2 tawny owls, predatory birds from the Galicia (northwest Spain). The PAHs found most abundantly were pyrene, fluoranthene, benzo[a]anthracene, and anthracene. Chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[ghi]perylene, and indeno[1,2,3-cd]pyrene were not detected. Intestine, kidney, and lung were more polluted than other tissues.

High Performance Liquid Chromatography with Fluorescence and Ultraviolet Detection of Polynuclear Aromatic Hydrocarbons in Barley Malt

1982 ◽  
Vol 65 (6) ◽  
pp. 1395-1402
Author(s):  
Frank L Joe ◽  
Jean Salemme ◽  
Thomas Fazio
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Alumina Column ◽  
Reverse Phase ◽  
Barley Malt ◽  
Relative Standard

Abstract A simple, rapid method has been developed for the separation and determination of polynuclear aromatic hydrocarbons (PAHs) in barley malt. An ultrasonic- cyclohexane extraction method was used to separate the PAHs from ground barley malt. The cyclohexane extracts were purified by chromatography through a water-deactivated silica gel-alumina column. The eluate from the column was concentrated and purified further by partitioning between dimethyl sulfoxide (DMSO) and cyclohexane. The DMSO extract was diluted with water and the PAHs were extracted back into cyclohexane. The cyclohexane extract was washed with water, dried through sodium sulfate, and evaporated, and the resulting residue was dissolved in 80% aqueous acetonitrile-methanol (1 + 1) and subjected to reverse phase high performance liquid chromatography. Thirty barley malt samples were analyzed using this procedure. Peaks having the same retention time as the carcinogen benzo(a)pyrene were isolated from 18 of the samples, and were equivalent to trace levels ranging from <0.1 to 0.2 ppb. Average recoveries of 11 PAHs, including benzo(a)pyrene, benzo(b)fluoranthene, indeno(l,2,3-cd)pyrene, and benz(a)anthracene, added to 25 g samples at 2.5 and 5 ppb, ranged from 78 to 97%, with a mean relative standard deviation of 6.6%.

Sign in / Sign up

Export Citation Format

Share Document