Quantitation of Ochratoxin A: Use of Reverse Phase Thin-Layer Chromatography for Sample Cleanup Followed by Liquid Chromatography or Direct Fluorescence Measurement

1988 ◽  
Vol 71 (5) ◽  
pp. 949-953 ◽  
Author(s):  
Andrzej A Frohlich ◽  
Ronald R Marquardt ◽  
Aniko Bernatsky
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ochratoxin A ◽  
Uv Light ◽  
Packed Column ◽  
Fluorescence Measurement ◽  
Reverse Phase ◽  
Direct Fluorescence ◽  
Layer Chromatography

Abstract An improved procedure for sample preparation and quantitation of ochratoxin A in moldy grain was developed. Grain samples were acidified and extracted, and extracts were subjected to reverse phase thin-layer chromatography (RPTLC). The separated spots, which were identified under UV light, were scraped and collected into recovery devices. Ochratoxin A was eluted from the adsorbent and analyzed by either liquid chromatography with fluorescence detection or direct spectrofluorometric measurement. The method yielded values that were proportional to the concentrations of toxin in the sample, had relatively high levels of recovery (94%), and required considerably less volume of solvents and preparation time than a standard packed column method.

Identification and Estimation of the Alkaloids of Rauwolfia serpentina by High Performance Liquid Chromatography and Thin Layer Chromatography

1983 ◽  
Vol 66 (4) ◽  
pp. 867-873
Author(s):  
Ugo R Cieri
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Uv Light ◽  
Extraction Procedures ◽  
Rauwolfia Serpentina ◽  
Aoac Method ◽  
Layer Chromatography

Abstract A procedure is presented for the identification and estimation of some of the alkaloids of Rauwolfia serpentina by high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). Two extraction procedures were studied, one essentially similar to the current AOAC method, and one using warm methanol. For HPLC, a μPorasil column, methanol as eluting solvent, and a fluorometric delector were used. Rescinnamine was detected at 330 nm, at which wavelength reserpine fluorescence is negligible. Reserpine was detected at 280 nm, where rescinnamine fluorescence is small. Other alkaloids detected were raubasinine, ajmalicine, yohimbine, ajmaiine, and serpentine. For TLC, CHCI3-CH3OH 197+ 3) and CHCI3-CH3OH (80 + 20) were used as developing solvents and spots were detected under long- and shortwave UV light. A semiquantitative IICprocedure was also developed for serpentine, the content of which was found to be in the 0.2-0.25% range.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

Sign in / Sign up

Export Citation Format

Share Document