Liquid Chromatographic Determination of Miconazole Nitrate in Creams and Suppositories

1989 ◽  
Vol 72 (3) ◽  
pp. 442-444
Author(s):  
Theodore A Tyler ◽  
Judith A Genzale
Keyword(s):  
Active Ingredient ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Absorbance Detection ◽  
Miconazole Nitrate ◽  
Liquid Chromatographic Determination ◽  
The Mean ◽  
Liquid Chromatographic

Abstract A rapid method has been developed for the determination of miconazole nitrate in creams and suppositories. The sample is dissolved in ethanol, diluted in acetonitrile-water (1 + 1), and injected onto a C18 column. The mobile phase consists of 55% acetonitrile, a triethylammonium phosphate buffer, and an ion-pairing agent. The total run time is less than 4 min, and the active ingredient is determined using absorbance detection at 214 nm. The mean recovery of miconazole from spiked placebo samples was 99.7 ± 0.7% for the cream samples at the 2% level and 98.8 ± 0.3% for the suppository samples at the 4% level.

Liquid Chromatographic Determination of Sodium Saccharin, Caffeine, Aspartame, and Sodium Benzoate in Cola Beverages

1984 ◽  
Vol 67 (4) ◽  
pp. 745-747 ◽  
Author(s):  
Theodore A Tyler
Keyword(s):  
Rapid Method ◽  
Sodium Benzoate ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Absorbance Detection ◽  
Sodium Saccharin ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid method has been developed for the determination of sodium saccharin, caffeine, aspartame, and sodium benzoate in cola beverages. The sample is degassed, diluted in water, and injected onto a C18 column. The mobile phase consists of 15% acetonitrile in triethylammonium phosphate buffer adjusted to pH 4.3 with NaOH. The total run time is less than 10 min and the active compounds are determined using absorbance detection at 214 nm.

scholarly journals Liquid Chromatographic Determination of Dehydroepiandrosterone (DHEA) in Dietary Supplement Products

2000 ◽  
Vol 83 (4) ◽  
pp. 847-858 ◽  
Author(s):  
Richard D Thompson ◽  
Marvin Carlson
Keyword(s):  
Adrenal Cortex ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Mean Value ◽  
Peripheral Tissues ◽  
Complex Product ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method was developed to assess the potency of products that contain dehydroepiandrosterone (DHEA), a precursor hormone synthesized from cholesterol by the human adrenal cortex and converted to potent androgens and/or estrogens in peripheral tissues. Forty-five commercial products (both single and multi-ingredient) were subjected to analysis by the proposed method. A Zorbax Rx C18 column with a mobile phase containing acetonitrile–0.025M phosphate buffer (60 + 40), pH 3.50, and UV detection at 292 nm was used for 87% of the products. An alternative mobile phase containing methanol–0.025M phosphate (75 + 25), pH 3.50, was used to isolate DHEA from more complex product mixtures. Assay values varied from 0 to 109.5% of the declared amount with an overall mean value of 91.1%. The recoveries based on fortified products ranged from 96.4 to 101.2%, and the intraday precision (RSD, n = 5) varied from 0.50 to 1.66%.

Improved liquid-chromatographic determination of haloperidol in plasma.

1984 ◽  
Vol 30 (7) ◽  
pp. 1228-1230 ◽  
Author(s):  
A K Dhar ◽  
H Kutt
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Room Temperature ◽  
Internal Standard ◽  
Isoamyl Alcohol ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract This method for determination of haloperidol in plasma is based on "high-performance" isocratic liquid chromatography with the use of a C8 bonded reversed-phase column at room temperature. Haloperidol and the internal standard (chloro-substituted analog) are extracted from alkalinized plasma into isoamyl alcohol/heptane (1.5/98.5 by vol) and back-extracted into dilute H2SO4. The aqueous phase is directly injected onto the column. The mobile phase is a 30/45/25 (by vol) mixture of phosphate buffer (16.5 mmol/L, pH 7.0), acetonitrile, and methanol. Unlike other liquid-chromatographic procedures for haloperidol, commonly used psychotropic drugs do not interfere. Analysis can be completed within an hour. The procedure is extremely sensitive (1.0 microgram/L) and is well reproducible (CV 5.6% for a 2.5 micrograms/L concentration in plasma).

Liquid Chromatographic Determination of Diazepam in Tablets: Collaborative Study

1985 ◽  
Vol 68 (3) ◽  
pp. 545-546
Author(s):  
Michael Tsougros
Keyword(s):  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Photometric Detection ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
The Mean ◽  
Liquid Chromatographic

Abstract A stability indicating liquid chromatographic method for the determination of diazepam in tablets was collaboratively studied by 6 laboratories. The method uses a Cig reverse phase column, a methanolwater mobile phase, p-tolualdehyde as the internal standard, and photometric detection at 254 nm. The collaborators were supplied with a synthetic tablet powder and 3 commercial tablet samples. The mean recovery of diazepam from the synthetic tablet powder was 100.2%. For all samples analyzed, the coefficient of variation was < 1.5%. The method has been adopted official first action.

Simultaneous Liquid Chromatographic Determination of Thiamine and Riboflavin in Selected Foods

1993 ◽  
Vol 76 (5) ◽  
pp. 1156-1160 ◽  
Author(s):  
Art Sims ◽  
Dirk Shoemaker
Keyword(s):  
American Association ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Fluorescence Detector ◽  
Liquid Chromatographic Determination ◽  
Optimum Wavelength ◽  
The Mean ◽  
Sensitivity Data ◽  
Liquid Chromatographic

Abstract A reliable, improved liquid chromatographic (LC) method has been developed for the measurement of thiamine and riboflavin in foods. The major improvement in the method is the chromatographic separation achieved. The method is also very reproducible and extremely sensitive. After autoclave extraction, samples are derivatized to form thiochrome (a highly fluorescent oxidation product of thiamine). Riboflavin is naturally fluorescent. Interferences are removed on a Ci8 cartridge and chromatographed by using a reversed-phase separation. The mobile phase used is 72% 0.005M NH4OAc (pH 5.0)-28% MeOH. Fluorescence detection using wavelength switching, 370-435 for thiamine and 370-520 for riboflavin, allows determination of each vitamin at its optimum wavelength for maximum sensitivity. Detection limits were 0.05 ng for both thiamine and riboflavin. The method can also be performed by using a fluorescence detector at a single wavelength, but with a sacrifice of sensitivity. Data comparisons between AOAC fluorometric and LC results were excellent for routine samples, as well as for American Association of Cereal Chemists (AACC) check samples. All LC results from AACC samples were within 2 standard deviations of the mean. Reproducibility was 1.9% for thiamine and 1.6% for riboflavin.

Liquid Chromatographic Determination of Carbofuran in Technical and Formulated Products: Collaborative Study

1986 ◽  
Vol 69 (5) ◽  
pp. 915-918
Author(s):  
Edward J Kikta ◽  
◽  
E Bane ◽  
A Burns ◽  
A Christensen ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Data Sets ◽  
Matched Pairs ◽  
Reverse Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method for the analysis of technical and formulated carbofuran samples was evaluated in a collaborative study. Carbofuran is determined by reverse phase LC, using a water-methanol mobile phase and acetophenone as internal standard, and detected at 280 nm. Twelve samples, 5 formulations and technical matched pairs, were analyzed by 17 collaborating laboratories. Accuracy and variability of results are typical of large LC data sets. The method has been adopted official first action.

Liquid Chromatographic Determination of Ergot Alkaloids in Wheat

1986 ◽  
Vol 69 (4) ◽  
pp. 697-699
Author(s):  
George M Ware ◽  
Allen S Carman ◽  
Octave J Francis ◽  
Shia S Kuan
Keyword(s):  
Mobile Phase ◽  
Ergot Alkaloids ◽  
Ammonium Phosphate ◽  
Ammonium Hydroxide ◽  
Chromatographic Determination ◽  
Resin Column ◽  
Fluorescence Detector ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is described for the determination of individual ergot alkaloids in wheat. The sample is extracted with ethyl acetate-4% ammonium hydroxide (100 + 10), and the extract is cleaned up by liquidliquid partition. The ergot alkaloids are resolved by liquid chromatography (LC), using a porous cross-linked polystyrene-divinylbenzene resin column and a mobile phase consisting of acetonitrile-0.05M dibasic ammonium phosphate (55 + 45) buffered at pH 10.0. The ergot alkaloids ergonovine, ergonovinine, ergotamine, ergotaminine, α-ergocryptine, α-ergocryptinine, ergocristine, and ergocristinine are separated by LC and detected with a fluorescence detector. Recovery of ergot alkaloids added to wheat at levels of 16-760 ng/g averaged 85.6% with a coefficient of variation of 11.1%.

scholarly journals Micellar Liquid Chromatographic Determination of Carbaryl and 1-Naphthol in Water, Soil, and Vegetables

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mei-Liang Chin-Chen ◽  
Maria Rambla-Alegre ◽  
Abhilasha Durgbanshi ◽  
Devasish Bose ◽  
Sandeep K. Mourya ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Limit Of Detection ◽  
Cetylpyridinium Chloride ◽  
Sodium Dodecyl ◽  
Chromatographic Determination ◽  
Limit Of Quantification ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

A liquid chromatographic procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite 1-naphthol, using a C18 column (250’mm’ × ’4.6’mm) with a micellar mobile phase and fluorescence detection at maximum excitation/emission wavelengths of 225/333’nm, respectively. In the optimization step, surfactants sodium dodecyl sulphate (SDS), Brij-35 andN-cetylpyridinium chloride monohydrate, and organic solvents propanol, butanol, and pentanol were considered. The selected mobile phase was 0.15’M SDS-6% (v/v)-pentanol-0.01’M NaH2PO4buffered at pH 3. Validation studies, according to the ICH Tripartite Guideline, included linearity (r>0.999), limit of detection (5 and 18’ng mL-1, for carbaryl and 1-naphthol, resp.), and limit of quantification (15 and 50’ng mL-1, for carbaryl and 1-naphthol, resp.), with intra- and interday precisions below 1%, and robustness parameters below 3%. The results show that the procedure was adequate for the routine analysis of these two compounds in water, soil, and vegetables samples.

scholarly journals Liquid Chromatographic Determination of Mebendazole and its Metabolites, Aminomebendazole and Hydroxymebendazole, in Eel Muscle Tissue

1996 ◽  
Vol 79 (3) ◽  
pp. 645-651 ◽  
Author(s):  
Christiaan A J Hajee ◽  
Nel Haagsma
Keyword(s):  
Muscle Tissue ◽  
Mobile Phase ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Extraction Column ◽  
Phase Extraction ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract An analytical method is presented for liquid chromatographic (LC) determination of mebendazole (MBZ), hydroxymebendazole (MBZ-OH), and aminomebendazole (MBZ-NH2) in eel muscle tissue. Muscle tissue is extracted with ethyl acetate at pH 7.5. After addition of n-hexane, the extract is cleaned up and concentrated on an aminopropyl solid-phase extraction column. The test solutions are analyzed isocratically on a ChromSpher B LC column with acetonitrile–phosphate buffer, pH 6.2, as mobile phase. Limits of detection and quantitation were 0.7 and 1.1 ¼g/kg, respectively, for MBZOH; 1.4 and 2.3 ¼g/kg, respectively, for MBZ; and 1.5 and 2.1 ¼g/kg, respectively, for MBZ-NH2. Interand intraday coefficients of variation were 3.5 and 3.4%, respectively, for MBZ-OH; 2.5 and 3.1%, respectively, for MBZ; and 5.8 and 4.8%, respectively, for MBZ-NH2. Mean recoveries were 90% for MBZ, 74% for MBZ-NH2, and 92% for MBZ-OH. A linear range of applicability of at least 10–1000 ¼g/kg was found for each analyte. Incurred MBZ-NH2 (181.3 ¼g/kg) was identified in eel muscle tissue apart from MBZ (23.7 ¼g/kg) after 48 h exposure ina treatment bath containing MBZ at 1 mg/L.

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