Liquid Chromatographic Determination of Ivermectin in Bovine Milk: Interlaboratory Study
Abstract A laboratory trial was completed for an analytical method that can quantitate the marker residue of ivermectin, 22,23-dihydroavermectin B1a, in bovine milk at 1 ng/mL Currently, ivermectin is not approved for use in lactating dairy cows. In this method, the ivermectin residues are isolated from the milk matrix by a series of liquid-liquid extractions. A fluorescent derivative of the marker compound is prepared and then quantified by liquid chromatography with fluorescence detection. The interlaboratory study was successfully completed by using dosed milk and milk fortified with marker residue at 1,2, and 4 ng/mL. The average recoveries by the 3 participating laboratories were 87,59, and 95% at 1 ng/mL; 90,61, and 96% at 2 ng/mL; and 90,73, and 99% at 4 ng/mL. The concentrations of the marker residue in the dosed milk were 4.3, 3.7, and 4.7 ng/mL; coefficients of variation were 4.0,24.8, and 5.9%, respectively.