scholarly journals Headspace Gas Chromatographic Determination of 1,3-Butadiene in Simulated Saliva

2000 ◽  
Vol 83 (4) ◽  
pp. 929-932 ◽  
Author(s):  
Shirley Abrantes ◽  
Tereza C Dos Santos ◽  
Grazieli SimÕES
Keyword(s):  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Calibration Graph ◽  
Chewing Gum ◽  
Preliminary Assessment ◽  
Headspace Gas ◽  
Sensitive Determination ◽  
Headspace Gc ◽  
Migration Limit

Abstract A headspace gas chromatographic (GC) method was developed to determine 1,3-butadiene (1,3-BD) in simulated saliva in contact with chewing gum. The calibration graph was linear, and the limit of detection was 0.004 mg/L, which is well below the migration limit for this substance. The headspace GC method provides rapid and reliable analysis for monitoring 1,3-BD migration from chewing gum into simulated saliva. In this paper, we report headspace methodology for sensitive determination of 1,3-BD in chewing gum and results of selected analyses, enabling preliminary assessment of possible exposure to 1,3-BD through migration.

Headspace Gas Chromatographic Determination of Residual 1,3-Butadiene in Rubber-Modified Plastics and Its Migration from Plastic Containers into Selected Foods

1987 ◽  
Vol 70 (1) ◽  
pp. 18-21
Author(s):  
Timothy P Mcneal ◽  
Charles V Breder
Keyword(s):  
Chromatographic Determination ◽  
Chewing Gum ◽  
Flame Ionization ◽  
External Calibration Curve ◽  
Headspace Gas ◽  
The Matrix ◽  
Chromatographic Procedure ◽  
Standard Additions ◽  
Plastic Containers

Abstract A headspace gas chromatographic procedure has been developed for the determination of 1,3-butadiene in rubber-modified plastics and in some foods. Polymer solutions or foods are equilibrated in sealed vials at 90°C, and headspace samples are injected into a gas chromatograph. 1,3-Butadiene residues are measured using a flame ionization detector and are quantitated by the method of standard additions or an external calibration curve. Refrigerator tubs, vegetable oil bottles, chewing gum, and foods in contact with this type of packaging were analyzed. Limits of quantitation varied with the matrix, ranging from 2 ng/g (ppb) in chewing gum to 20 ng/g in polymers. 1,3-Butadiene was found in one polymer at 53 ng/g with an 8% coefficient of variation. The procedure yields "apparent" trace levels of 1,3-butadiene, and confirmation by a complementary technique is required.

Detection of peroxyacetyl nitrate in air using chemiluminescence aerosol detector

2014 ◽  
Vol 68 (11) ◽  
Author(s):  
Pavel Mikuška ◽  
Lukáš Bružeňák ◽  
Zbyněk Večeřa
Keyword(s):  
Alkaline Solution ◽  
Limit Of Detection ◽  
Packed Column ◽  
Direct Reaction ◽  
Peroxyacetyl Nitrate ◽  
Brij 35 ◽  
Alternative Approach ◽  
Sensitive Determination ◽  
Triton X

AbstractA method for the rapid and sensitive determination of peroxyacetyl nitrate (PAN) in air based on a chemiluminescence reaction with an alkaline solution of luminol in the chemiluminescence aerosol detector is described. The PAN is chromatographically separated from nitrogen dioxide and ozone in a packed column filled with 5 % OV-1 on Chromosorb 30/60 and the eluted PAN is detected via the direct reaction with the luminol solution consisting of 0.002 mol L−1 luminol, 1 vol. % Brij-35 and 0.1 mol L−1 KOH. The limit of detection is 14.9 ng m−3 (3 ppt) of PAN. Alternatively, the PAN after separation is thermally converted to NO2 which is detected by the chemiluminescence reaction with a solution consisting of 0.002 mol L−1 luminol, 0.5 mol L−1 KOH, 0.2 mol L−1 Na2SO3, 0.1 mol L−1 KI, 0.05 mol L−1 EDTA and 0.5 vol. % triton X-100. The alternative approach affords the simultaneous determination of PAN and NO2. The limit of detection is 50 ppt of PAN and 50 ppt of NO2. The time resolution is 3 min. The method was applied to the measurement of ambient peroxyacetyl nitrate in air.

Headspace gas chromatographic determination of ethylene oxide in air

1986 ◽  
Vol 21 (12) ◽  
pp. 701-704 ◽  
Author(s):  
R. Binetti ◽  
S. Di Marzio ◽  
P. Di Prospero ◽  
L. Gramiccioni ◽  
G. Viviano ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chromatographic Determination ◽  
Headspace Gas

scholarly journals Liquid Chromatographic Determination of α-Solasonine in Frozen Green Peas as an Indicator of the Presence of Nightshade Berries

2003 ◽  
Vol 86 (4) ◽  
pp. 759-763 ◽  
Author(s):  
Peter Cavlovic ◽  
Mohan Mankotia ◽  
Peter Pantazopoulos ◽  
Peter M Scott
Keyword(s):  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Solanum Nigrum ◽  
Expanded Uncertainty ◽  
Method Performance ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Green Peas

Abstract Nightshade berries containing glycoalkaloids can be a contaminant in green peas. Methodology was developed to detect this contamination. The glycoalkaloid α-solasonine was extracted from frozen green peas with 1% (v/v) acetic acid, cleaned up on a C18 cartridge, and determined by liquid chromatography with UV detection at 200 nm. Method performance characteristics for the determination of α-solasonine include linearity from 140 to 1500 ng injected (r = 0.9996–0.9999); recovery ranging from 68 to 79%; limit of quantitation (LOQ) = 4.5 ppm (280 ng standard), and limit of detection = 0.64 ppm (40 ng standard). At the LOQ, the expanded uncertainty at 95% confidence was 0.38 × the reported value. The method was applied to the detection of α-solasonine in frozen green peas in a 2-year study of 60 samples of frozen green peas from Ontario, Canada. None of the samples contained α-solasonine. No unripe berries of Solanum nigrum were detected visually in the samples.

Gas-Liquid Chromatographic Determination of T-2 Toxin in Plasma

1983 ◽  
Vol 66 (4) ◽  
pp. 909-912 ◽  
Author(s):  
Steven P Swanson ◽  
Venkatachalam Ramaswamy ◽  
Val R Beasley ◽  
William B Buck ◽  
Harold H Burmeister
Keyword(s):  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Aqueous Sodium Hydroxide ◽  
Chromatographic Determination ◽  
Florisil Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed onOV-1 at 230°C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 ± 5.5% at levels ranging from 50 to 1000 ng/m L. The limit of detection is 25 ng/mL.

Rapid Thin Layer Chromatographic Determination of Aflatoxin M1 in Powdered Milk

1985 ◽  
Vol 68 (5) ◽  
pp. 952-954
Author(s):  
Maria Luisa Serralheiro ◽  
Maria Lurdes Quinta
Keyword(s):  
Aqueous Solution ◽  
Carbon Tetrachloride ◽  
Thin Layer ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Methanol Solution ◽  
Aflatoxin M1 ◽  
Powdered Milk ◽  
Layer Chromatography

Abstract A method has been developed for the detection of aflatoxin Mi in milk. The toxin is extracted with chloroform, the extract is evaporated, and the residue is partitioned between carbon tetrachloride and an aqueous saline-methanol solution. The toxin is once again extracted with chloroform from the methanol solution and analyzed by thin layer chromatography. The limit of detection of Mi in powdered milk is 0.5 μg/ kg; recoveries of added Mj are about 83%. The limit of detection can be improved to 0.3 μg/kg if the plate is sprayed with an aqueous solution of H2S04 after development.

Liquid Chromatographic Determination of Benzoyl Peroxide in Acne Preparations

1984 ◽  
Vol 67 (5) ◽  
pp. 913-915
Author(s):  
Chih-Kuang Chou ◽  
David C Locke
Keyword(s):  
Benzoyl Peroxide ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Detector Response ◽  
Electrochemical Detector ◽  
Reverse Phase ◽  
Order Of Magnitude ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid, precise, and accurate liquid chromatographic (LC) method is described for the determination of benzoyl peroxide (BP) in acne preparations. BP is extracted from a water dispersion of the preparation with dichloromethane (DCM), and an aliquot is eluted from a C-18 reverse phase LC column with acetonitrile-O.lOM aqueous NaCI04. Selective and sensitive quantitation is accomplished with a reductive mode electrochemical detector. This detector is an order of magnitude more sensitive than a 240 nm UV absorption detector; the lower limit of detection is 2 ng for a 4 μL injection. The recovery of BP is 99.4% and the detector response is linear to at least 2 μg per 4 μL injection.

Gas-chromatographic determination of 5-fluorocytosine in human serum.

1976 ◽  
Vol 22 (6) ◽  
pp. 772-776 ◽  
Author(s):  
S A Harding ◽  
G F Johnson ◽  
H M Solomon
Keyword(s):  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Mean Value ◽  
Normal Serum ◽  
Trimethylsilyl Derivative ◽  
Serum Samples ◽  
Gas Chromatographic Method

Abstract We describe a sensitive and precise gas-chromatographic method, in which cytosine is used as the internal standard, for determination of an antifungal agent, 5-fluorocytosine, in serum. The trimethylsilyl derivative of this drug is well separated from the internal standard and from normal serum constituents. Amphotericin B does not interfere with the determination of 5-fluorocytosine. The lower limit of detection for 5-fluorocytosine is 1 mg/liter when 200 mul of serum is analyzed. Within-run precision (CV), established by analysis of 10 replicates, was 4.5% at a concentration of 19.9 mg/liter. Twenty-five serum samples were analyzed for 5-fluorocytosine by a microbiological assay and by the gas-chromatographic method. Mean value observed with the bioassay was 78.5 mg/liter and with our procedure was 69.4 mg/liter. When values for our assay were regressed against values for the bioassay, slope of the least-squares line was 0.85, intercept was 2.7 mg/liter, and r was 0.93.

Thin Layer Chromatographic Determination of Citrinin

1979 ◽  
Vol 62 (1) ◽  
pp. 201-202 ◽  
Author(s):  
Robert D Stubblefield
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Linear Relationship ◽  
Silica Gel ◽  
Coefficient Of Variation ◽  
Ethylenediaminetetraacetic Acid ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Tlc Plates

Abstract Clearly defined zones of citrinin can be obtained on thin layer chromatographic (TLC) plates and measured by fluorodensitometry. Silica gel plates were prepared as a slurry with aqueous 0.05M Na2EDTA (ethylenediaminetetraacetic acid), spread at 0.5 mm wet thickness, and activated at 105°C for 1 hr. Plates were developed in acetic acid-benzene (5+95). The limit of detection was 10 ng citrinin/zone. Densitometric analysis (365 nm excitation, 505 nm emission) revealed that a linear relationship exists for levels of 10 ng to at least 100 ng/zone wtih a coefficient of variation of ±5%.

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