Improved Filter Paper Test for Detecting and Quantifying Increased Esterase Activity in Organophosphate-Resistant Mosquitoes (Diptera: Culicidae)

1989 ◽  
Vol 82 (2) ◽  
pp. 347-353 ◽  
Author(s):  
Nicole Pasteur ◽  
George P. Georghiou
2002 ◽  
Vol 129 (1) ◽  
pp. 139-145 ◽  
Author(s):  
E. C. DUARTE ◽  
T. W. GYORKOS ◽  
L. PANG ◽  
S. ÁVILA ◽  
C. J. F. FONTES

The ring-infected erythrocyte surface antigen (RESA), is one of the falciparum malaria vaccine candidates rarely studied in Brazil. Fieldwork logistics to conduct serology studies is simplified when eluates from whole blood dried on filter paper can be used. Therefore, this study aimed to assess the inter-test reliability for the anti-RESA ELISA-based indices using eluates from filter paper and from serum samples. The study population consisted of 210 individuals (Brazil) from whom matched samples were collected. Anti-RESA ELISA-based index means (±S.D.) were 15.29% (±28.13%) for filter paper and 11.79% (±23.67%) for serum samples. The intra-class correlation coefficient was estimated to be 82.38%, indicating high test reliability. However, there was a significant tendency for filter paper test results to have higher values than serum sample test results (P<0.001). Explanations for this finding may be the presence of haemoglobin in the eluates from filter paper, which may interfere with ELISA testing.


2011 ◽  
Vol 90-93 ◽  
pp. 701-706
Author(s):  
Jing Song Qian ◽  
Hang Lu

The soil-water characteristic defines the relationship between the soil suction and gravimetric water content, w, or the volumetric water content, θ, or the degree of saturation, S. It is a convenient method to predict water content in the subgrade using the curve. But in the field tests of subgrades, the compaction degree of soil became lower with time than initially designed. With the purpose of finding out effect of compaction degree on soil-water characteristic curve, a study to the SWCC (soil-water characteristic curve) of Chongming low liquid limit clay using filter paper method was carried out and is presented in this paper. Specimens of different water contents were prepared by absorbing different amount of water, in order to better simulate the process of wetting of subgrade soil. After the filter paper test, the soil-water characteristic curve was fitted with two models, and then the effect of compaction degree on the curve was analyzed. The figures show that the compaction degree of the specimen will decrease with higher water content, and from the gravimetric water content-matric suction curve, it is found that compaction degree has an effect on air-entry value and water storage capacity.


Heart ◽  
2010 ◽  
Vol 97 (2) ◽  
pp. 131-136 ◽  
Author(s):  
A. A. Hagege ◽  
E. Caudron ◽  
T. Damy ◽  
R. Roudaut ◽  
A. Millaire ◽  
...  

2021 ◽  
Author(s):  
Elham Samadi Kalkhoran ◽  
Mohammad Taghi Alebrahim ◽  
Mohammad Taghi Alebrahim ◽  
Hamid Reza Mohammaddust Chamn Abad ◽  
Jens Carl Streibig ◽  
...  

Abstract Frequent use of herbicides might impose a risk for nontarget species, such as earthworms. The objective was to test the combined toxic effect of binary herbicide mixtures: metribuzin:halosulfuron and metribuzin: flumioxazin on a nontarget earthworm in two test systems. Two experiments on filter paper and two on artificial soil determined lethality and chronic toxicity on the earthworm. Results showed the flumioxazin had no high toxicity in contact filter paper test, with the lethal concentration (LC50) of 153.10 µg a.i cm-2 at 48 h. In the artificial soil test, flumioxazin LC10 was 0.65 mg a.i kg-1 on day 14. Metribuzin showed high toxicity to earthworms in the filter paper test with LC50 17.17 µg a.i cm-2 at 48 h but LC10 of metribuzin. Halosulfuron and metribuzin were highly toxic with LC10 value 0.24 and 0.47 mg a.i kg-1 on day 14. The herbicides' mixed effect showed in both test systems antagonistic effect, meaning that the mixtures retracted the herbicides' action in the earthworms relative to a Concentration Addition reference model. A chronic toxicity test showed that earthworm catalase was stimulated by metribuzin:halosulfuron (50:50%) and metribuzin (100%).


2021 ◽  
Author(s):  
Wenwu Chen ◽  
Quanquan Jia ◽  
Peng Liu ◽  
Yanmei Tong

Abstract Loess is very widely distributed, and the unsaturated hydraulic conductivity of loess is related to many engineering issues. In order to determine the unsaturated hydraulic conductivity of remolded loess more conveniently and at a lower cost, filter paper test and soil column seepage test were carried out. The results indicate that in the one-dimensional soil column seepage process, the unsaturated hydraulic conductivity of loess increases with the increase of the volumetric water content, and as the seepage time continues, the unsaturated hydraulic conductivity of loess at different depths gradually becomes uniform. The changes in the microstructure indicate that the collapsible settlement will occur during the seepage process, which will reduce the unsaturated hydraulic conductivity of the underlying loess to a certain extent. Compared with the experimental results, the soil hydraulic conductivity curve (SHCC) obtained by the van Genuchten-Mualem model (VG-M model) underestimates the magnitude of unsaturated hydraulic conductivity in the part with a low volumetric water content (< 20%). and the Childs ༆ Collis-George model (CCG model) has more consistent results with the experimental results because it is based on more segments of the soil-water characteristic curve (SWCC).


HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 302-304 ◽  
Author(s):  
Muharrem Ergun ◽  
Ellen T. Paparozzi ◽  
Dermot P. Coyne ◽  
Durward Smith ◽  
Stephen Kachman ◽  
...  

Seedcoat color is an important trait, as it affects marketing and consumer acceptance of pinto beans (Phaseolus vulgaris L.). Pinto breeding line NE 94-4 showed seedcoat yellowing in on-farm field trials in Nebraska in 1996 and 1997. Hail, sprinkler irrigation, and fall rainfall appeared to be involved in increasing seedcoat yellowing, based on analysis of field and weather data of on-farm trial sites. The objective of this study was to determine the effect of moisture on seedcoat yellowing of pinto line NE 94-4 (susceptible) and pinto `UI-114' (highly resistant). Two greenhouse experiments were conducted involving misting of bean plants near maturity and injecting water into maturing bean pods. Another experiment evaluated the response of seeds of these two bean entries to moisture by placing them on moist filter paper in petri dishes in the laboratory. Results showed that both genotype and moisture content are involved in seedcoat yellowing. This simple, cheap, and effective filter paper test was then used to evaluate seedcoat yellowing of nine pinto genotypes in response to moisture. Pinto NE 94-4 and `Kodiak' showed the greatest change, while `Bill Z' showed the least change, in seedcoat color.


Sign in / Sign up

Export Citation Format

Share Document