Soybean Foliage Consumption Reduces Adult Western Corn Rootworm (Diabrotica virgifera virgifera)(Coleoptera: Chrysomelidae) Survival and Stimulates Flight

2021 ◽  
Author(s):  
Joseph L Spencer ◽  
Timothy R Mabry ◽  
Eli Levine ◽  
Scott A Isard
Keyword(s):  
Wind Tunnel ◽  
Crop Rotation ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Egg Laying ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Egg Maturation ◽  
Behavioral Resistance ◽  
Collection Sites

Abstract Western corn rootworm, Diabrotica virgifera virgifera LeConte, biology is tied to the continuous availability of its host (corn, Zea mays L.). Annual rotation of corn with a nonhost, like soybean (Glycine max (L.) Merrill) was a reliable tactic to manage western corn rootworm. Behavioral resistance to annual crop rotation (rotation resistance) allowed some eastern U.S. Corn Belt populations to circumvent rotation by laying eggs in soybean and in cornfields. When active in soybean, rotation-resistant adults commonly consume foliage, in spite of detrimental effects on beetle survival. Rotation-resistant beetle activity in soybean is enabled by the expression of certain proteinases and an adapted gut microbiota that provide limited protection from soybean antiherbivore defenses. We investigated the effects of corn and soybean herbivory on rotation-resistant female survival and initiation of flight using mortality assays and wind tunnel flight tests. Among field-collected females tested with mortality assays, beetles from collection sites in a cornfield survived longer than those from collection sites in a soybean field. However, reduced survival due to soybean herbivory could be restored by consuming corn tissues. Field-collected beetles that fed on a soybean tissue laboratory diet or only water were more likely to fly in a wind tunnel than corn-feeding beetles. Regardless of collection site and laboratory diet, 90.5% of beetles that flew oriented their flights upwind. Diet-related changes in the probability of flight provide a proximate mechanism for interfield movement that facilitates restorative feeding and the survival of females previously engaged in soybean herbivory. Rotation-resistant western corn rootworm females feeding on soybean tissues experience reduced survival in mortality assays and display increased flight probability (which may facilitate flight back to a cornfield where consumption of host tissues improves survival potential and facilitates maturation of eggs). The consequences of soybean herbivory provide a proximal mechanism for behavioral resistance to crop rotation. Increased egg-laying probability while feeding on soybean tissues, facilitation of egg maturation while feeding on corn tissues, and interfield movement are previously documented consequences.

Oviposition site selected by the western corn rootworm (Diabrotica virgifera virgifera Leconte) in southern Ontario strip plots

2005 ◽  
Vol 85 (4) ◽  
pp. 949-954 ◽  
Author(s):  
C. C. Knight ◽  
K. P. Pauls ◽  
M. K. Sears ◽  
A. W. Schaafsma
Keyword(s):  
Insect Pest ◽  
Adult Emergence ◽  
Diabrotica Virgifera Virgifera ◽  
Conclusive Evidence ◽  
Western Corn Rootworm ◽  
Egg Laying ◽  
Larval Feeding ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Southern Ontario

Western corn rootworm (WCR), Diabrotica virgifera virgifera L., is the most serious insect pest of corn, Zea mays L., in North America. Crop rotation had successfully controlled this pest until the late 1980s when a new biotype that lays its eggs in non-corn crops was discovered in Illinois. This biotype has spread to parts of Indiana, Michigan, and Ohio, and threatens Ontario. By using emergence cages, Pherocon Am® sticky traps and corn root ratings, egg-laying behaviour of WCR in Ontario was assessed in strip crop settings that represent typical cropping systems of southern Ontario. WCR adults were active in both soybean and winter wheat fields in July and August. Larval feeding and adult emergence in first-year corn fields following soybean and wheat were similar, confirming that oviposition did occur in both crops. These data do not, however, provide conclusive evidence for the presence of the rotation resistant WCR in Ontario because previous studies have demonstrated that WCR are capable of utilizing other crops for oviposition. Key words: Western corn rootworm, Diabrotica virgifera virgifera, strip plots, corn, soybean, wheat (winter)

Incidence of Western Corn Rootworm Beetles (Coleoptera: Chrysomelidae) on Corn in Virginia from 1987 to 1992

1993 ◽  
Vol 28 (1) ◽  
pp. 136-141 ◽  
Author(s):  
Roger R. Youngman ◽  
Eric R. Day
Keyword(s):  
Crop Rotation ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
The State ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Continuous Corn ◽  
Corn Field ◽  
Survey Results ◽  
Diabrotica Virgifera Virgifera Leconte

The discovery of western corn rootworm beetles, Diabrotica virgifera virgifera LeConte, in a southwest Virginia corn field in 1985 prompted annual surveys of corn fields in an average of 28 counties across the state from 1987 to 1992. All counties included in the annual surveys were representative of the major corn-growing regions of Virginia. Survey results indicated that western corn rootworm beetles spread rapidly throughout most of the western and central continuous corn-growing regions of the state. In the eastern and southeastern corn-growing regions of the state, where crop rotation is widely practiced, detections of western corn rootworm beetles were less common and typically involved only one to two counties per year from 1987 to 1992.

scholarly journals Management of western corn rootworm (Diabrotica virgifera virgifera)

2012 ◽  
Vol 27 (3) ◽  
pp. 189-201 ◽  
Author(s):  
Ivan Sivcev ◽  
Petar Kljajic ◽  
Miroslav Kostic ◽  
Lazar Sivcev ◽  
Sladjan Stankovic
Keyword(s):  
Crop Rotation ◽  
Diabrotica Virgifera Virgifera ◽  
Sowing Date ◽  
Climatic Conditions ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Significant Area ◽  
Soil Insecticides ◽  
Diabrotica Virgifera Virgifera Leconte

Western corn rootworm (WCR) was registered for the first time in Europe near the Surcin international airport in Serbia in 1992. The spread of WCR on the territory of Serbia and its population density increased fast. The Serbian territory was entirely populated in the following few years, while major damages occurred on corn grown for two or more years in the same field. Data on damages caused to over 140,000 ha under corn until 1999 were collected by organized monitoring. After 2000 and 2003, population abundance of D.v. virgifera, as well as the number of damaged corn fields, significantly decreased due to drought and application of crop rotation. Corn rootworm has one generation per year. It overwinters in the egg stage. Under the climatic conditions of Serbia larvae hatching starts around May 15th. The highest number of larvae on root is observed around June 20th when feeding is most intensive and plants become lodged as they lose roots. First adults emerge by the end of June. Their abundance increases during July and reaches maximum by the end of the month. From the second decade of August the abundance decreases. Adults are present in the field until the first frosts. Larvae are much more harmful and significant than adults. Larvae feed on roots or into roots by boring. Roots can be entirely destroyed under heavy attack and the host plants lodged already at the end of June. Under our climatic and agrotechnical conditions, adults are sporadic pests. Adults are a threat only when sowing is done after the optimal sowing date or in case of stubble corn sowing. Crop rotation is an efficient and most widespread means of WCR control. No damage on corn grown in crop rotation has been registered in Serbia for now. In the first year of production corn does not require protection from Diabrotica virgifera virgifera LeConte larvae. Several insecticides have performed high efficacy by application at sowing and have been registered for commercial use. On the other hand, soil insecticides have never been applied on a significant area in Serbia.

scholarly journals Detection of alternative splicing in Diabrotica virgifera virgifera LeConte, in association with Bt resistance using RNA-seq and PacBio Iso-Seq

2020 ◽  
Author(s):  
Zixiao Zhao ◽  
Christine G. Elsik ◽  
Bruce E. Hibbard ◽  
Kent S. Shelby
Keyword(s):  
Alternative Splicing ◽  
Matrix Protein ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Rna Seq ◽  
Bt Resistance ◽  
Bt Toxins ◽  
Alternatively Spliced

AbstractBackgroundAlternative splicing is one of the major mechanisms that increases transcriptome diversity in eukaryotes, including insect species that have gained resistance to pesticides and Bt toxins. In western corn rootworm (Diabrotica virgifera virgifera LeConte), neither alternative splicing nor its role in resistance to Bt toxins has been studied.ResultsTo investigate the mechanisms of Bt resistance we carried out single-molecule real-time (SMRT) transcript sequencing and Iso-seq analysis on resistant, eCry3.1Ab-selected and susceptible, unselected, western corn rootworm neonate midguts which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. We present transcriptome-wide alternative splicing patterns of western corn rootworm midgut in response to feeding on eCry3.1Ab-expressing corn using a comprehensive approach that combines both RNA-seq and SMRT transcript sequencing techniques. We found that 67.73% of multi-exon genes are alternatively spliced, which is consistent with the high transposable element content of the genome. One of the alternative splicing events we identified was a novel peritrophic matrix protein with two alternative splicing isoforms. Analysis of differential exon usage between resistant and susceptible colonies showed that in eCry3.1Ab-resistant western corn rootworm, expression of one isoform was significantly higher than in the susceptible colony, while no significant differences between colonies were observed with the other isoform.ConclusionOur results provide the first survey of alternative splicing in western corn rootworm and suggest that the observed alternatively spliced isoforms of peritrophic matrix protein may be associated with eCry3.1Ab resistance in western corn rootworm.

scholarly journals Inheritance and Fitness Costs of Cry3Bb1 Resistance in Diapausing Field Strains of Western Corn Rootworm (Coleoptera: Chrysomelidae)

2020 ◽  
Vol 113 (6) ◽  
pp. 2873-2882
Author(s):  
Ram B Shrestha ◽  
Aaron J Gassmann
Keyword(s):  
Developmental Rate ◽  
Diabrotica Virgifera Virgifera ◽  
Dry Mass ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Adult Size ◽  
Inheritance Of Resistance ◽  
Diabrotica Virgifera ◽  
Fitness Costs ◽  
Diabrotica Virgifera Virgifera Leconte

Abstract Field-evolved resistance to Cry3Bb1 corn by western corn rootworm, Diabrotica virgifera virgifera LeConte (Colleoptera: Chrysomellidae), has been reported in field populations in Iowa, Illinois, Nebraska, and Minnesota. Inheritance and fitness costs associated with Cry3Bb1 resistance have been determined for non-diapausing laboratory strains of western corn rootworm with either laboratory-selected resistance or field-derived resistance. However, information on inheritance and fitness costs of Cry3Bb1 resistance in the diapausing field populations is lacking. In this study, we determined the inheritance of Cry3Bb1 resistance for four diapausing field strains of western corn rootworm using plant-based bioassays. We also determined the fitness costs for eight diapausing field populations in a greenhouse experiment. We found that Cry3Bb1 resistance was an autosomal trait and that the inheritance of resistance was mostly non-recessive; however, there was some variation in the dominance of Cry3Bb1 resistance. We did not find evidence of fitness costs affecting survival to adulthood, developmental rate, or adult dry mass. However, we did detect a fitness cost affecting adult size. The results of this study will add to the current understanding of field-evolved resistance to Cry3Bb1 corn by western corn rootworm and help in developing better strategies to manage resistance.

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