Production of Near-Isogenic Lines and Marked Monosomic Lines in Common Wheat (Triticum aestivum) cv. Chinese Spring

Abstract Sixteen near-isogenic lines (NILs) carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of common wheat (Triticum aestivum) cv. Chinese Spring (CS). Three genes from alien species showed segregation distortion. In NILs carrying a marker gene of rye (Secale cereale) or Aegilops caudata, the alien chromosome segments were detected by fluorescence in situ hybridization (FISH). The NILs were grown with replications and the effect of marker genes on plant morphology in the genetic background of CS was investigated. These NILs were further crossed with the corresponding monosomics of CS and 13 monosomic lines whose monosome carries a respective marker gene were established and named “marked monosomics.” Many of the marked monosomics were distinguishable from the disomic NILs because of the different dosage effect of the genes. The NILs are utilized for studies on gene isolation or gene regulation. Marked monosomics are useful not only for monosomic analysis but also for production of homologous chromosome substitution lines because chromosome observation is not required.

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Analysis of spike productivity traits in near-isogenic lines of the common wheat cultivar Saratovskaya 29 carrying alien marker genes

Russian Journal of Genetics ◽

10.1134/s1022795410040058 ◽

2010 ◽

Vol 46 (4) ◽

pp. 417-424 ◽

Cited By ~ 2

Author(s):

V. S. Arbuzova ◽

T. T. Efremova ◽

L. I. Laikova

Keyword(s):

Common Wheat ◽

Wheat Cultivar ◽

Marker Genes ◽

Near Isogenic Lines ◽

Common Wheat Cultivar ◽

Isogenic Lines ◽

The Common

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Dissection and validation of a QTL cluster linked to Rht-B1 locus controlling grain weight in common wheat (Triticum aestivum L.) using near-isogenic lines

Theoretical and Applied Genetics ◽

10.1007/s00122-020-03622-z ◽

2020 ◽

Vol 133 (9) ◽

pp. 2639-2653

Author(s):

Panfeng Guan ◽

Xueyi Shen ◽

Qing Mu ◽

Yongfa Wang ◽

Xiaobo Wang ◽

...

Keyword(s):

Triticum Aestivum ◽

Common Wheat ◽

Triticum Aestivum L ◽

Grain Weight ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

Qtl Cluster

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Using near-isogenic lines of common wheat (Triticum aestivum L.) to determine the relationship between GlutoPeak parameters and quality characteristics

Cereal Research Communications ◽

10.1007/s42976-019-00004-0 ◽

2019 ◽

Vol 48 (1) ◽

pp. 81-86

Author(s):

W. F. Song ◽

X. X. Zhang ◽

Y. B. Zhang ◽

L. Chen ◽

X. F. Yang ◽

...

Keyword(s):

Triticum Aestivum ◽

Common Wheat ◽

Triticum Aestivum L ◽

Quality Characteristics ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

The Relationship

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The influence of etephon on yield components of common wheat (Triticum aestivum L.) cv. Bezostaya isogenic lines with Rht genes

Annales UMCS Agricultura ◽

10.2478/v10081-008-0034-1 ◽

2008 ◽

Vol 63 (3) ◽

Author(s):

Krzysztof Kowalczyk ◽

Agnieszka Jakubczak ◽

Michał Nowak

Keyword(s):

Triticum Aestivum ◽

Common Wheat ◽

Yield Components ◽

Triticum Aestivum L ◽

Isogenic Lines ◽

Rht Genes

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Comparative analysis of genetic background in eight near-isogenic wheat lines with different H genes conferring resistance to Hessian fly

Genome ◽

10.1139/g10-095 ◽

2011 ◽

Vol 54 (1) ◽

pp. 81-89 ◽

Cited By ~ 8

Author(s):

S. S. Xu ◽

C. G. Chu ◽

M. O. Harris ◽

C. E. Williams

Keyword(s):

Genetic Background ◽

Genetic Similarity ◽

Triticum Aestivum L ◽

Hessian Fly ◽

Pedigree Analysis ◽

Recurrent Parent ◽

Linkage Drag ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

H Gene

Near-isogenic lines (NILs) are useful for plant genetic and genomic studies. However, the strength of conclusions from such studies depends on the similarity of the NILs’ genetic backgrounds. In this study, we investigated the genetic similarity for a set of NILs developed in the 1990s to study gene-for-gene interactions between wheat ( Triticum aestivum L.) and the Hessian fly ( Mayetiola destructor (Say)), an important pest of wheat. Each of the eight NILs carries a single H resistance gene and was created by successive backcrossing for two to six generations to susceptible T. aestivum ‘Newton’. We generated 256 target region amplification polymorphism (TRAP) markers and used them to calculate genetic similarity, expressed by the Nei and Li (NL) coefficient. Six of the NILs (H3, H5, H6, H9, H11, and H13) had the highly uniform genetic background of Newton, with NL coefficients from 0.97 to 0.99. However, genotypes with H10 or H12 were less similar to Newton, with NL coefficients of 0.86 and 0.93, respectively. Cluster analysis based on NL coefficients and pedigree analysis showed that the genetic similarity between each of the NILs and Newton was affected by both the number of backcrosses and the genetic similarity between Newton and the H gene donors. We thus generated an equation to predict the number of required backcrosses, given varying similarity of donor and recurrent parent. We also investigated whether the genetic residues of the donor parents that remained in the NILs were related to linkage drag. By using a complete set of ‘Chinese Spring’ nullisomic-tetrasomic lines, one third of the TRAP markers that showed polymorphism between the NILs and Newton were assigned to a specific chromosome. All of the assigned markers were located on chromosomes other than the chromosome carrying the H gene, suggesting that the genetic residues detected in this study were not due to linkage drag. Results will aid in the development and use of near-isogenic lines for studies of the functional genomics of wheat.

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Chilling injury and chlorotic reactions of euploids and aneuploids of the group 6 homoeologues of common wheat

Genome ◽

10.1139/g92-069 ◽

1992 ◽

Vol 35 (3) ◽

pp. 468-473 ◽

Cited By ~ 1

Author(s):

Ernest D. P. Whelan ◽

G. B. Schaalje

Keyword(s):

Triticum Aestivum ◽

Common Wheat ◽

Chinese Spring ◽

Prolonged Exposure ◽

Chilling Injury ◽

Triticum Aestivum L ◽

Recessive Trait ◽

Cytoplasmic Effects ◽

Group 6 ◽

Winter Wheat Cultivars

Aneuploid seedlings of the common wheat (Triticum aestivum L.) cv. Chinese Spring (CS) that are nullisomic or telosomic for the long arm of chromosome 6D are susceptible to chilling injury under prolonged exposure to 6 °C; normal euploids or telosomics for the short arm are not. Studies of seedling grown for various durations at 20 °C prior to growth at 6 °C showed that chilling injury was a juvenile phenomenon and that the extent of injury was inversely proportional to the duration of growth at 20 °C to a maximum of about 14 days. When reciprocal crosses were made between susceptible 6D nullisomics or long-arm ditelocentrics of CS and resistant 6D nullisomics of three spring and one winter wheat cultivars, progenies from aneuploid F1 hybrids all segregated for susceptibility as a recessive trait and at a frequency approximating a dihybrid ratio; no cytoplasmic effects were detected. Aneuploids of the group 6 homoeologues of the spring wheat cvs. Cadet and Rescue were resistant, as were group 6 whole-chromosome substitutions of eight different donor wheats in the recipient parent CS and 56 other euploids tested. Genes for resistance to chilling injury appear to involve the group 6 chromosomes and the short arm of 6D in Chinese Spring. In contrast with chilling injury, all aneuploid lines with only four doses of the "corroded" loci on group 6 chromosomes exhibited chlorotic symptoms.Key words: Triticum aestivum, chilling injury.

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