scholarly journals Collection and Rearing of Container Mosquitoes and a 24-h Addition to the CDC Bottle Bioassay

2020 ◽  
Vol 20 (6) ◽  
Author(s):  
Casey Parker
Keyword(s):  
Aedes Aegypti ◽  
Disease Control ◽  
Mosquito Species ◽  
Insecticide Susceptibility ◽  
Resistance Monitoring ◽  
Standardized Protocol ◽  
Container Mosquitoes ◽  
Control And Prevention ◽  
Container Mosquito ◽  
Susceptibility Status

Abstract Container mosquitoes (Diptera: Culicidae) oviposit their eggs in both natural and artificial containers. Many container mosquito species also serve as important vectors of disease-causing pathogens including Aedes aegypti, Ae. albopictus, and Ae. triseriatus. Control of these species can be done through the use of adulticide sprays. The efficacy of these treatments is highly dependent on the insecticide susceptibility status of the local mosquito populations. This paper provides protocols on collecting and rearing container mosquitoes for use in the Centers for Disease Control and Prevention (CDC) bottle bioassay. A brief description of the CDC bottle bioassay is provided as well as a standardized protocol for the incorporation of a 24-h mortality to the CDC bottle bioassay. Results from this 24-h holding addition to the CDC bottle bioassay reveal that some forms of resistance may be missed without the incorporation of the additional mortality reading. These protocols provide a foundation for new laboratories to establish rearing protocols and begin conducting resistance monitoring.

scholarly journals Risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of aedes aegypti (linnaeus) in some sites in Accra, Ghana

2016 ◽  
Vol 50 (3) ◽  
pp. 136-141
Author(s):  
Takashi Suzuki ◽  
Joseph H Osei ◽  
Sasaki Sasaki ◽  
Michelle Adimazoya ◽  
Maxwell Appawu ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Emerging Diseases ◽  
Research Network ◽  
Insecticide Susceptibility ◽  
Aedes Mosquitoes ◽  
Human Landing ◽  
Survey Results ◽  
The University ◽  
University Of Ghana ◽  
Susceptibility Status

Background: Dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. Although, Dengue has not been reported in Ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease. Objective: This study was carried on the University of Ghana campus to determine the risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of Ae. aegypti in some sites in Accra, Ghana. Design: Larval surveys were carried to inspect containers within households and estimate larval indices and adult Aedes mosquitoes were collected using human landing collection technique. WHO tube assays was used to assess the insecticide susceptibility status of Aedes mosquitoes. Results: Ae. aegypti were the most prevalent species, 75.5% and followed by Ae. vittatus, 23.9 %. Ae. albopictus and Ae. granti were in smaller numbers. Household index (HI), Breteau index (BI), and container index were calculated as 8.2%, 11.2% and 10.3% respectively with man-vector contact rate of 0.67 bites/man-hour estimated for the area. The mortalities recorded for Ae. aegypti from WHO tube assays was 88%, 94%, 80% and 99% for DDT (4%), deltamethrin (0.05%), lambdacyhalothrin (0.05%) and permethrin (0.75%) respectively.Conclusion: The survey results indicated that the density of Aedes mosquitoes was considered to be sufficient to promote an outbreak of viral haemorrhagic fevers on Legon Campus. Aedes mosquitoes were found to be resistant to DDT, deltamethrin and lamdacyhalothrin, but susceptible to permethrin. Funding: This study was supported in part by Japan Initiative for Global Research Network on Infectious Diseases(J-Grid).Keywords: Mosquitoes, Aedes aegypti, insecticide, risk, VHF transmission, Ghana

The flight distances of floodwater mosquitoes (Aedes vexans, Ochlerotatus sticticus and Ochlerotatus caspius) in Osijek, Eastern Croatia

Biologia ◽  
2011 ◽  
Vol 66 (4) ◽  
Author(s):  
Mirta Bogojević ◽  
Enrih Merdić ◽  
Tomislav Bogdanović
Keyword(s):  
Disease Control ◽  
Mosquito Species ◽  
Release Site ◽  
Maximum Distance ◽  
Dispersal Rate ◽  
Aedes Vexans ◽  
Dispersal Pattern ◽  
Mark Release Recapture ◽  
Control And Prevention

AbstractIn spring 2004, the mark-release-recapture study was conducted in the Osijek area, covering the total of 171 km2, to describe dispersal pattern of three floodwater mosquito species (Aedes vexans, Ochlerotatus sticticus and Oc. caspius). Forty CO2 baited Center for Disease Control and Prevention (CDC) traps were set at distances varying from 0.95 to 16.7 km from release site. Fifty thousand mosquitoes were released, and a total of 22 marked mosquitoes were recaptured in 12 traps, 82% of which were collected in the first six days after release. The maximum distance of recaptured mosquito (Oc. sticticus) was found at 11.68 km from the release site. During the study, the average dispersal rate per day for mosquitoes ranged from 0.96 km to 3.6 km in regard to different species.

scholarly journals Insecticide susceptibility status in Mexican populations ofStegomyia aegypti(= Aedes aegypti): a nationwide assessment

2017 ◽  
Vol 32 (2) ◽  
pp. 162-174 ◽  
Author(s):  
P. A. KURI-MORALES ◽  
F. CORREA-MORALES ◽  
C. GONZÁLEZ-ACOSTA ◽  
M. MORENO-GARCIA ◽  
R. SANTOS-LUNA ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Susceptibility ◽  
Susceptibility Status ◽  
Mexican Populations

scholarly journals A Survey of the Mosquito Species in Maxcanu, Yucatan, Mexico

2018 ◽  
Vol 34 (2) ◽  
pp. 128-130 ◽  
Author(s):  
Roger Cauich-Kumul ◽  
Juana María Coronado-Blanco ◽  
Jorge Ruiz-Ruiz ◽  
Maira Segura-Campos ◽  
Edgar Koyoc-Cardeña ◽  
...  
Keyword(s):  
Species Richness ◽  
Aedes Aegypti ◽  
Random Sampling ◽  
Rainy Season ◽  
Mosquito Species ◽  
Simple Random Sampling ◽  
Abundant Species ◽  
Anopheles Albimanus ◽  
Control And Prevention ◽  
Rural Locality

ABSTRACT A survey was carried out to identify the mosquitoes inhabiting human premises in the rural locality of Maxcanú, Yucatán, Mexico. Using the Centers for Disease Control and Prevention backpack aspirators, simple random sampling was carried out inside of 101 homes during the November 2013 rainy season. A total of 1,492 specimens were collected. Three subfamilies (Anophelinae, Culicinae, and Toxorhynchitinae) and 5 species were identified: Anopheles albimanus, Aedes aegypti, Culex interrogator, Limatus durhamii, and Toxorhynchites theobaldi. The most abundant species was Cx. interrogator (74%) followed by Ae. aegypti (25%). The Chao 1 and Bootstrap species richness estimator indicated that it was possible to collect 90% of the expected species. This is the 1st time that the presence of An. albimanus, Cx. interrogator, Li. durhamii, and Tx. theobaldi has been recorded in Maxcanú.

scholarly journals A New Record of Aedes thibaulti In Massachusetts

2019 ◽  
Vol 35 (3) ◽  
pp. 207-209
Author(s):  
Ellen P. Bidlack ◽  
Kaitlyn M. O'Donnell ◽  
Douglas S. Bidlack ◽  
Todd B. Duval ◽  
Curtis Best ◽  
...  
Keyword(s):  
Disease Control ◽  
New Record ◽  
Mosquito Species ◽  
Forested Wetlands ◽  
Larval Habitat ◽  
Light Traps ◽  
Control And Prevention ◽  
The City ◽  
Collection Sites ◽  
Wetland Types

ABSTRACT For the 1st time, collections of adult female Aedes thibaulti are reported from Massachusetts. Initial collections occurred in 2016 and again in 2017 and 2018. This mosquito was found at 35 locations within 28 municipalities, including the city of Boston. Most of the Ae. thibaulti were collected with the Centers for Disease Control and Prevention miniature light traps baited with CO2. Collections were made from epidemiological (EPI) wk 22 to 35 with a peak at EPI wk 24. Although larvae have not yet been collected, the most common wetland types adjacent to collection sites were forested wetlands, which is consistent with the known larval habitat of this mosquito species. Aedes thibaulti is likely established in the state of Massachusetts.

scholarly journals Entomological Investigation of Aedes aegypti In Neighborhoods With Confirmed Human Arbovirus Infection In Puerto Rico

2018 ◽  
Vol 34 (3) ◽  
pp. 233-236 ◽  
Author(s):  
Gilberto E. Felix ◽  
Roberto Barrera ◽  
Jesus Vazquez ◽  
Kyle R. Ryff ◽  
Jorge L. Munoz-Jordan ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Aedes Aegypti ◽  
Disease Control ◽  
Vertical Transmission ◽  
Mosquito Density ◽  
Infection Rates ◽  
Single Location ◽  
Control And Prevention ◽  
Arbovirus Infection ◽  
The Relationship

ABSTRACT The exotic arboviruses chikungunya (CHIKV) and Zika (ZIKV) recently caused large outbreaks and continue to circulate in Puerto Rico, prompting entomological investigations at 9 locations with confirmed CHIKV- or ZIKV-infected human cases. Adult mosquitoes were collected using the Centers for Disease Control and Prevention autocidal gravid ovitraps over a 14-day period at each site. Mean female Aedes aegypti captured per trap-week ranged from 13.47 per trap-week to 1.27 per trap-week. Arbovirus-positive pools were detected at 7 of the 9 sampling sites. We investigated vertical transmission by collecting Ae. aegypti eggs in a single location where ZIKV was found in adult mosquitoes. We discuss the relationship between vector density and infection rates and its implications for determining mosquito density thresholds of novel invasive arboviruses such as CHIKV and ZIKV.

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