Characterization of the Ryanodine Receptor Gene With a Unique 3′-UTR and Alternative Splice Site From the Oriental Fruit Moth

B52 is a Drosophila melanogaster protein that plays a role in general and alternative splicing in vitro. It is homologous to the human splicing factor ASF/SF2 which is essential for an early step(s) in spliceosome assembly in vitro and also regulates 5' and 3' alternative splice site choice in a concentration-dependent manner. In vitro, B52 can function as both a general splicing factor and a regulator of 5' alternative splice site choice. Its activity in vivo, however, is largely uncharacterized. In this study, we have further characterized B52 in vivo. Using Western blot (immunoblot) analysis and whole-mount immunofluorescence, we demonstrate that B52 is widely expressed throughout development, although some developmental stages and tissues appear to have higher B52 levels than others do. In particular, B52 accumulates in ovaries, where it is packaged into the developing egg and is localized to nuclei by the late blastoderm stage of embryonic development. We also overexpressed this protein in transgenic flies in a variety of developmental and tissue-specific patterns to examine the effects of altering the concentration of this splicing factor in vivo. We show that, in many cell types, changing the concentration of B52 adversely affects the development of the organism. We discuss the significance of these observations with regard to previous in vitro results.

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Prevalent skipping of an individual exon accounts for shortened protein 4.1 Presles

Blood ◽

10.1182/blood.v80.11.2925.2925 ◽

1992 ◽

Vol 80 (11) ◽

pp. 2925-2930 ◽

Cited By ~ 1

Author(s):

S Feddal ◽

S Hayette ◽

F Baklouti ◽

R Rimokh ◽

R Wilmotte ◽

...

Keyword(s):

Red Blood Cells ◽

Alternative Splice ◽

Splice Site ◽

Blood Cells ◽

Protein 4.1 ◽

Alternative Splice Site ◽

Alternatively Spliced ◽

Domain Protein

Abstract An asymptomatic shortened variant of protein 4.1 (-8.5 Kd) was first recognized in the red blood cells and designated protein 4.1 Presles. We show here that the missing segment belongs to the 22/24 Kd domain. Protein 4.1 cDNA from reticulocytes was amplified, mapped, and sequenced. The truncation appeared to result from the prevalent skipping of an individual and alternatively spliced exon, also called motif II, whereas this motif is preferentially retained under normal conditions. The same phenomenon was observed in lympho-blastoid cells. Sequencing over 80 bp of intronic sequences 5′ and 3′ of motif II failed to reveal any change. A new alternative splice site was incidently found 81 nucleotide downstream of motif II in both normal and truncated 4.1 mRNA.

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half pint Regulates Alternative Splice Site Selection in Drosophila

Developmental Cell ◽

10.1016/s1534-5807(02)00128-4 ◽

2002 ◽

Vol 2 (3) ◽

pp. 343-353 ◽

Cited By ~ 57

Author(s):

Cheryl Van Buskirk ◽

Trudi Schüpbach

Keyword(s):

Alternative Splice ◽

Splice Site ◽

Site Selection ◽

Splice Site Selection ◽

Alternative Splice Site

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CHARACTERIZATION OF THE SPLICE SITES IN GT–AG AND GC–AG INTRONS IN HIGHER EUKARYOTES USING FULL-LENGTH cDNAs

Journal of Bioinformatics and Computational Biology ◽

10.1142/s0219720004000570 ◽

2004 ◽

Vol 02 (02) ◽

pp. 309-331 ◽

Cited By ~ 21

Author(s):

SUMIE KITAMURA–ABE ◽

HITOMI ITOH ◽

TAKANORI WASHIO ◽

AKIHIRO TSUTSUMI ◽

MASARU TOMITA

Keyword(s):

Alternative Splice ◽

Splice Site ◽

Consensus Sequence ◽

Full Length ◽

Splice Sites ◽

Consensus Sequences ◽

Full Length Cdna ◽

Strong Consensus ◽

Higher Eukaryotes

For the purpose of analyzing the relation between the splice sites and the order of introns, we conducted the following analysis for the GT–AG and GC–AG splice site groups. First, the pre-mRNAs of H. sapiens, M. musculus, D. melanogaster, A. thaliana and O. sativa were sampled by mapping the full-length cDNA to the genomes. Next, the consensus sequences at different regions of pre-mRNAs were analyzed in the five species. We also investigated the mononucleotide and dinucleotide frequencies in the extensive regions around the 5' splice sites (5'ss) and 3' splice sites (3'ss). As a result, differential frequencies of nucleotides at the first 5'ss in both the GT–AG and GC–AG splice site groups were observed in A. thaliana and O. sativa pre-mRNAs. The trend, which indicates that GC 5'ss possess strong consensus sequences, was observed not only in mammalian pre-mRNAs but also in the pre-mRNAs of D. melanogaster, A. thaliana and O. sativa. Furthermore, we examined the consensus sequences of the constitutive and alternative splice sites. It was suggested that in the case of the alternative GC–AG introns, the tendency to have a weak consensus sequence at 5'ss is different between H. sapiens and M. musculus pre-mRNAs.

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