Susceptibility of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) to Temephos in Thailand and Surrounding Countries

2020 ◽  
Vol 57 (4) ◽  
pp. 1207-1220
Author(s):  
Manop Saeung ◽  
Ratchadawan Ngoen-Klan ◽  
Kanutcharee Thanispong ◽  
Vithee Muenworn ◽  
Michael J Bangs ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Virus Disease ◽  
Lao Pdr ◽  
Wide Distribution ◽  
Immature Stage ◽  
Published Data ◽  
Control Programs ◽  
Collection Sites

Abstract Aedes-borne virus disease control relies on insecticides to interrupt transmission. Temephos remains a key chemical for control of immature stage Aedes in Thailand and much of Southeast Asia. However, repeated use of insecticides may result in selection for resistance in vector populations, thus compromising operational intervention. Herein, the phenotypic response to temephos by Aedes aegypti (L.) and Aedes albopictus (Skuse) collected in Thailand and surrounding countries is presented. Data from 345 collection sites are included: 283 from literature review (244 sites with Ae. aegypti, 21 with Ae. albopictus, and 18 having both species sampled), plus 62 locations with Ae. aegypti in Thailand conducted between 2014 and 2018. Susceptibility assays followed WHO guidelines using the recommended discriminating dose of temephos (0.012 mg/liter) against late third to early fourth instar Ae. aegypti. Findings revealed 34 locations with susceptible Ae. aegypti, 13 with suspected resistance, and 15 indicating resistance. Published data between 1999 and 2019 in Thailand found Ae. aegypti resistant in 73 of 206 collection sites, whereas 3 locations from 11 sampled with low-level resistant in Ae. albopictus. From surrounding countries conducting temephos assays (Cambodia, Lao PDR, Myanmar, Malaysia, and Singapore), resistance is present in Ae. aegypti and Ae. albopictus from 27 of 56 and 19 of 28 locations, respectively. Routine insecticide susceptibility monitoring should be an operational requirement in vector control programs. Given the wide distribution and apparent increase in temephos-resistance, alternative larvicidal compounds must be considered if chemical control is to remain a viable vector control strategy.

scholarly journals Co-occurrence of kdr mutations V1016I and F1534C in pyrethroid-resistant Aedes aegypti (Diptera: Culicidae) populations from Costa Rica

2019 ◽  
Author(s):  
Aryana Zardkoohi ◽  
David Castañeda ◽  
Carmen Castillo ◽  
Juan C Lol ◽  
Francisco Lopez ◽  
...  
Keyword(s):  
Costa Rica ◽  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Vector Control ◽  
Resistance Management ◽  
Management Plan ◽  
Costa Rican ◽  
Mosquito Vector ◽  
Control Programs ◽  
Control And Prevention

AbstractAedes aegypti (Linnaeus, 1762) is considered the most important mosquito vector species for several arboviruses (e.g., dengue, chikungunya, Zika) in Costa Rica. The main strategy for the control and prevention of Aedes-borne diseases relies on insecticide-based vector control. However, the emergence of insecticide resistance in the mosquito populations present a big threat for the prevention actions. The characterization of the mechanisms driving the insecticide resistance in Ae. aegypti are vital for decision making in vector control programs. Therefore, we analyzed the voltage-gated sodium channel gene for the presence of the V1016I and F1534C kdr mutations in pyrethroid-resistant Ae. aegypti populations from Puntarenas and Limon provinces, Costa Rica. The CDC bottle bioassays showed that both Costa Rican Ae. aegypti populations were resistant to permethrin and deltamethrin. In the case of kdr genotyping, results revealed the co-occurrence of V1016I and F1534C mutations in permethrin and deltamethrin-resistant populations, as well as the fixation of the 1534C allele. Therefore, our findings make an urgent call to expand the knowledge about the insecticide resistance status and mechanisms in the Costa Rican populations of Ae. aegypti which must be a priority to develop an effective resistance management plan.

scholarly journals Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia

2021 ◽  
Vol 15 (3) ◽  
pp. e0009205
Author(s):  
Rosilawati Rasli ◽  
Yoon Ling Cheong ◽  
M. Khairuddin Che Ibrahim ◽  
Siti Futri Farahininajua Fikri ◽  
Rusydi Najmuddin Norzali ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Vector Control ◽  
Aedes Albopictus ◽  
World Health ◽  
Dengue Vector ◽  
Organophosphate Insecticide ◽  
Potential Threat ◽  
Pyrethroid Insecticides ◽  
Dengue Vector Control

Background In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.

scholarly journals Semi Field Trial Competitive Power Married Made Sterile Aedes Albopictus as Method of Vector Control: A Current Vector Controlling Method

2019 ◽  
Vol 3 (1) ◽  
pp. 172-180
Author(s):  
Ahmad Rosyadi ◽  
Ahmad Sehabudin ◽  
Ariel Rifayat
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Case Fatality Rate ◽  
Case Fatality ◽  
Hemorrhagic Fever ◽  
Current Vector ◽  
Disease Vector ◽  
Very High ◽  
A Current

Aedes albopictus is a plant that causes Dengue Hemorrhagic Fever is not as prevalent as Aedes Aegypti is not a disease given by Aedes albopictus mosquito is not much different from Aedes Aegypti mosquito. The Case Fatality Rate (CFR) of Dengue Fever is very high in Indonesia, at 41.4% at the beginning of dengue cases in Indonesia. Innovation continues to create for disease vector control to break the chain of disease. The use of sterile insect techniques is a useful thing in the things that need to be socialized in the community.

scholarly journals Phenotypic and molecular analysis of insecticide resistance in Aedes albopictus populations from Greece

2020 ◽  
Author(s):  
Sofia Balaska ◽  
Emmanouil Alexandros Fotakis ◽  
Ilias Kioulos ◽  
Linda Grigoraki ◽  
Spyridoula Mpellou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
Molecular Analysis ◽  
Aedes Albopictus ◽  
Pyrethroid Resistance ◽  
Disease Outbreaks ◽  
Control Methods ◽  
Resistance Mutations ◽  
Control Programs ◽  
Southern European Countries

Abstract Background: Aedes albopictus has a well-established presence in southern European countries, associated with recent disease outbreaks (e.g. Chikungunya). Development of insecticide resistance in the vector is a major concern as it’s control mainly relies on the use of biocides. Data on the specie’s resistance status is essential for efficient and sustainable control. Methods: We investigated the insecticide resistance status of several Ae. albopictus populations from Greece. Bioassays were performed against diflubenzuron (DFB), B. thuringiensis var. israelensis (Bti), deltamethrin and malathion. Molecular analysis of known insecticide resistance loci was performed, i.e. voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance; presence and frequency of carboxylesterases 3 (CCEae3a) and 6 (CCEae6a) gene amplification associated with organophosphate (OP) resistance and; chitin synthase-1 (CHS-1) for the possible presence of DFB resistance mutations. Results: Bioassays showed full susceptibility to DFB, Bti and deltamethrin, but resistance against the OP malathion. VGSC analysis revealed a widespread distribution of mutations F1534C (in all populations, with allelic frequencies between 6.6% - 68.3%), and I1532T (in 6 populations), but absence of V1016G. CCE gene amplifications were recorded in 8 out of 11 populations. Co-presence of mutation F1534C and CCEae3a amplification was reported in a subgroup of samples. No mutations at the CHS locus I1043 were detected. Conclusions: The results indicate: (i) the suitability of larvicides DFB and Bti for Ae. albopictus control in Greece, (ii) a possible incipient pyrethroid resistance due to the presence of kdr mutations and (iii) a possible reduced efficacy of OPs, in a scenario of re-introducing them for vector control. The study highlights the need for systematic resistance monitoring for developing and implementing appropriate evidence-based control programs. Key words: diagnostic, arbovirus, mosquito tiger, insecticide resistance, vector control, Europe

scholarly journals Resistance Status of Aedes albopictus (Skuse) on Malathion in Bengkulu City

2018 ◽  
Vol 7 (2) ◽  
pp. 113-119
Author(s):  
Dessy Triana ◽  
Sitti Rahmah Umniyati ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Adult Female ◽  
Dengue Hemorrhagic Fever ◽  
Endemic Area ◽  
Hemorrhagic Fever ◽  
Evaluation Report ◽  
Control Programs ◽  
Diagnostic Dose

Abstract Dengue Hemorrhagic Fever (DHF) is a disease caused by dengue virus and transmitted by Aedes aegypti mosquito as the main vector and Aedes albopictus as secondary vector. In 2016, Bengkulu City was one of 3 cities that experiencing DHF outbreaks in Indonesia. Insecticides malathion have been used since 1990 in bulk in DHF control programs in Bengkulu City and have not had an evaluation report on Aedes sp. vulnerability to malathion. The purpose of this research was to know the resistance status of Ae albopictus mosquitoes from endemic and sporadic areas of DHF in Bengkulu City to malathion. The sample of the study was the Ae. albopictus adult female mosquitoes which collected from endemic and sporadic areas of DHF in Bengkulu City with ovitrap installation. The resistance test was performed by CDC Bottle Bioassay method with malation 96% and 50 μg/ml diagnostic dose of. The results of this study showed 1% mortality on endemic area and 5% on sporadic area. Aedes albopictus of both areas were resistant to malathion.   Keywords: Ae. albopictus, bottle bioassay, malathion.   Abstrak Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Pada tahun 2016, Kota Bengkulu merupakan 1 dari 3 kota yang mengalami KLB DBD di Indonesia. Insektisida malation telah digunakan sejak tahun 1990 secara massal dalam program pengendalian DBD di Kota Bengkulu dan belum memiliki laporan evaluasi mengenai kerentanan Aedes sp terhadap malation. Tujuan penelitian untuk mengetahui status kerentanan nyamuk Ae. albopictus dari daerah endemis dan sporadis DBD Kota Bengkulu terhadap malation. Sampel penelitian adalah nyamuk betina dewasa Ae. albopictus yang dikoleksi dari daerah endemik dan sporadis DBD Kota Bengkulu dengan pemasangan ovitrap. Uji kerentanan dilakukan dengan metode CDC Bottle Bioassay dengan malation 96% dan dosis diagnostik 50 µg/ml. Hasil penelitian ini  menunjukkan mortalitas 1% untuk daerah endemis dan 5% untuk daerah sporadis. Aedes albopictus kedua daerah telah resisten terhadap malation.   Kata Kunci: Ae. albopictus, bottle bioassay, malation.

scholarly journals A New, Cheap, Easy to Use, Foldable and Portable Autocidal Ovitrap for Aedes Control at Community Level

2021 ◽  
pp. 9-28
Author(s):  
G. Carnevale ◽  
P. Carnevale
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Vaccination Campaign ◽  
Specific Treatment ◽  
Hemorrhagic Fever ◽  
Community Level ◽  
It Use ◽  
Operational Issues ◽  
Single Larva

Except for Yellow Fever there is still no operational vaccine, the Dengue vaccine (CYD-TDV, sold under the brand name Dengvaxia©) raised several issues and vaccination campaign were stopped. On the other hand there is still no specific treatment for the main arbovirus transmitted by Aedes aegypti and Aedes albopictus such as dengue, Chikungunya, Zika and recent outbreaks occurred all over the World. For WHO “vector control is the key strategy to control or prevent the transmission of dengue (and other arbovirus) but clearly there is a need for improved new vector control approaches. Only another one vaccine is available against arbovirus is against Japanese Encephalitis transmitted by Culex tritaeniorhynchus. In line with this recommended approaches of new tools to be used at community level we successfully developed and implemented in Angola a “Very Simple Ovitrap Model” which actually “collected” eggs of Aedes aegypti warning for risk of arbovirus outbreaks, which, unfortunately, occurred few years later. These ovitraps were transformed as a tool for vector control in adding few drops of Bacillus thuringiensis (Bt) in the water and several hundred of tests showed that not a single larva of Aedes aegypti was ever found in these “Bt fitted simple ovitrap”. To solve some operational issues (transport) we tested in Bali during 3 years several size and color of foldable container prepared as ovitraps and put in a garden among grass, banana trees etc, we thus collected eggs of Aedes albopictus, important vector of dengue and dengue hemorrhagic fever (DHF), a serious threat in Asia, and preliminary tests (unpub.) showed some resistance to insecticide increasing the need for control. According to these trials, made in complete field conditions, we proposed a new foldable model of ovitrap as a vector control tool presented as a kit to be used at community level with a 3 steps action: open it/fill it/use it.

scholarly journals Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

2020 ◽  
Author(s):  
Sofia Balaska ◽  
Emmanouil Alexandros Fotakis ◽  
Ilias Kioulos ◽  
Linda Grigoraki ◽  
Spyridoula Mpellou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Pyrethroid Resistance ◽  
Disease Outbreaks ◽  
Evidence Based ◽  
Resistance Mutations ◽  
Molecular Monitoring ◽  
Allelic Frequencies ◽  
Control Programs

Abstract Background: Aedes albopictus has a well-established presence in southern European countries, associated with recent disease outbreaks (e.g. Chikungunya). Development of insecticide resistance in the vector is a major concern as its control mainly relies on the use of biocides. Data on the species’ resistance status is essential for efficient and sustainable control. To date the insecticide resistance status of Ae. albopictus populations from Greece against major insecticides used in vector control remains largely unknown. Methods: We investigated the insecticide resistance status of nineteen Ae. albopictus populations from 11 regions of Greece. Bioassays were performed against diflubenzuron (DFB), B. thuringiensis var. israelensis (Bti), deltamethrin and malathion. Known insecticide resistance loci were molecularly analysed, i.e. voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance; presence and frequency of carboxylesterases 3 (CCEae3a) and 6 (CCEae6a) gene amplification associated with organophosphate (OP) resistance and; chitin synthase-1 (CHS-1) for the possible presence of DFB resistance mutations. Results: Bioassays showed full susceptibility to DFB, Bti and deltamethrin, but resistance against the OP malathion (range of mortality: 55.30-91.40%). VGSC analysis revealed a widespread distribution of mutations F1534C (in all populations, with allelic frequencies between 6.6% - 68.3%), and I1532T (in 6 populations; allelic frequencies below 22.70%), but absence of V1016G. CCE gene amplifications were recorded in 8 out of 11 populations (overall frequency: 33%). Co-presence of mutation F1534C and CCEae3a amplification was reported in 39 of the 156 samples commonly analysed. No mutations at the CHS-1 locus I1043 were detected. Conclusions: The results indicate: (i) the suitability of larvicides DFB and Bti for Ae. albopictus control in Greece, (ii) possible incipient pyrethroid resistance due to the presence of kdr mutations and (iii) possible reduced efficacy of OPs, in a scenario of re-introducing them for vector control. The study highlights the need for systematic resistance monitoring for developing and implementing appropriate evidence-based control programs.

scholarly journals Review of the ecology and behaviour of Aedes aegypti and Aedes albopictus in Western Africa and implications for vector control

2021 ◽  
pp. 100074
Author(s):  
Beatrice R. Egid ◽  
Mamadou Coulibaly ◽  
Samuel Kweku Dadzie ◽  
Basile Kamgang ◽  
Philip J. McCall ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Aedes Albopictus ◽  
Western Africa
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