Measurement of the repeat period of myelin sheath using ultrathin frozen sections

2002 ◽  
Vol 51 (3) ◽  
pp. 195-197 ◽  
Author(s):  
T. Osawa
Keyword(s):  
Myelin Sheath ◽  
Frozen Sections ◽  
Ultrathin Frozen Sections

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

The Application of Ultracryotomy to Immunocytochemistry

1973 ◽  
Vol 31 ◽  
pp. 704-709
Author(s):  
R. G. Painter ◽  
K. T. Tokuyasu ◽  
S. J. Singer
Keyword(s):  
Frozen Sections ◽  
Protein Antigens ◽  
Carbon Coated ◽  
Antibody Conjugates ◽  
Ultrathin Frozen Sections

A technique for localizing intracellular antigens with immunoferritin conjugates directly on ultrathin frozen sections of glutaraldehyde-fixed tissues has been developed. This method overcomes some of the limitations of previously described procedures, since it avoids drastic fixation, dehydration and embedding procedures which could denature many protein antigens.Briefly cells or tissues were fixed with glutaraldehyde (0.5 to 2% for 1 hr), and ultrathin frozen sections were cut and mounted on grids covered with carbon-coated Formvar film by the procedure described previously. Such sections were stained with ferritin-antibody conjugates by methods described elsewhere.

Ultrathin frozen sections of yeast without aldehyde prefixation

1978 ◽  
Vol 36 (2) ◽  
pp. 58-59
Author(s):  
K. J. Böhm ◽  
a. E. Unger
Keyword(s):  
Aqueous Solutions ◽  
Biological Material ◽  
Yeast Cells ◽  
Frozen Sections ◽  
Good Preservation ◽  
Ultrathin Frozen Sections

During the last years it was shown that also by means of cryo-ultra-microtomy a good preservation of substructural details of biological material was possible. However the specimen generally was prefixed in these cases with aldehydes.Preparing ultrathin frozen sections of chemically non-prefixed material commonly was linked up to considerable technical and manual expense and the results were not always satisfying. Furthermore, it seems to be impossible to carry out cytochemical investigations by means of treating sections of unfixed biological material with aqueous solutions.We therefore tried to overcome these difficulties by preparing yeast cells (S. cerevisiae) in the following manner:

Immunogold localisation of endogenous immunoglobulin-G in ultrathin frozen sections of the human placenta

1989 ◽  
Vol 257 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Lopa Leach ◽  
Bryan M. Eaton ◽  
J. Anthony Firth ◽  
Soli F. Contractor
Keyword(s):  
Human Placenta ◽  
Immunoglobulin G ◽  
Frozen Sections ◽  
Immunogold Localisation ◽  
Ultrathin Frozen Sections

Ultrathin frozen sections of yeast cells

1972 ◽  
Vol 40 (1-2) ◽  
pp. 197-204 ◽  
Author(s):  
Heinz Bauer ◽  
Elsje Sigarlakie
Keyword(s):  
Yeast Cells ◽  
Frozen Sections ◽  
Ultrathin Frozen Sections

A study of ultrathin frozen sections of granular cells in newborn rat epidermis

1980 ◽  
Vol 70 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Shigeo Kakimi ◽  
Kimie Fukuyama ◽  
William L. Epstein
Keyword(s):  
Granular Cells ◽  
Frozen Sections ◽  
Newborn Rat ◽  
Ultrathin Frozen Sections

scholarly journals Visualization of longitudinally-oriented intermediate filaments in frozen sections of chicken cardiac muscle by a new staining method.

1983 ◽  
Vol 97 (2) ◽  
pp. 562-565 ◽  
Author(s):  
K T Tokuyasu
Keyword(s):  
Skeletal Muscle ◽  
Cardiac Muscle ◽  
Intermediate Filaments ◽  
Ethyl Cellulose ◽  
Staining Method ◽  
Uranyl Acetate ◽  
Frozen Sections ◽  
Cell Biol ◽  
Ultrathin Frozen Sections

When ultrathin frozen sections of chicken cardiac muscle were osmicated, dehydrated in ethanol, embedded in ethyl cellulose, and stained with acidic uranyl acetate, filaments of 10-12 nm width were visualized in wide interfibrillar spaces. Immunostaining of the frozen sections for desmin resulted in exclusive labeling of such filaments. These observations indicated that longitudinally oriented networks of intermediate filaments were present in the interfibrillar spaces, in addition to the transversely oriented networks that surround myofibrils at the level of Z band. As in skeletal muscle (Tokuyasu, K. T., A. H. Dutton, and S. J. Singer, 1983, J. Cell Biol. 97:1727-1735), desmin in chicken cardiac muscle is believed to be largely, if not entirely, in the form of intermediate filaments.

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