Studies on Human Cancer Using Sponge-Matrix Tissue Culture. III. The Invasive Properties of a Carcinoma (Strain He La) as Influenced by Temperature Variations, by Conditioned Media, and in Contact with Rapidly Growing Chick Embryonic Tissue

The fact that viral susceptibility changes during embryogenesis has been pointed out by both experimental embryologists and clinical practitioners, not to mention virologists working with avian material. In attempts to find the fundamental factors which make embryonic tissue susceptible or resistant to a given virus, the metabolic and proliferative rate have been considered relevant (Williamson et al., 1953; Robertson et al., 1955; Töndury, 1956). Experience accumulated in studies of the replication of various viruses in tissue culture has taught us that a high metabolic rate and active proliferation may not always enhance viral replication (Ginsberg, 1958). However, there seems to be justification for the view that an injurious agent leads to more severe tissue damage when it exercises its effect upon actively proliferating tissues than when it does so at the ‘resting stage’.

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Metabolic Gradients in Tissue Culture Studies of Human Cancer Cells

Human Tumor Cells in Vitro ◽

10.1007/978-1-4757-1647-4_9 ◽

1975 ◽

pp. 241-265 ◽

Cited By ~ 7

Author(s):

Joseph Leighton ◽

Ruy Tchao

Keyword(s):

Tissue Culture ◽

Cancer Cells ◽

Human Cancer ◽

Culture Studies ◽

Human Cancer Cells

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Eine Komplementbindungsreaktion zum Nachweis der bei Leukämieviren verschiedener Säuger vorkommenden gemeinsamen antigenen Komponente

Zeitschrift für Naturforschung B ◽

10.1515/znb-1970-0925 ◽

1970 ◽

Vol 25 (9) ◽

pp. 1029-1036 ◽

Cited By ~ 31

Author(s):

Werner Schäfer ◽

Jens Lange ◽

Liselotte Pister ◽

Eveline Seifert ◽

Fernando de Noronha ◽

...

Keyword(s):

Tissue Culture ◽

Cancer Cells ◽

Human Cancer ◽

Leukemia Virus ◽

Mouse Leukemia ◽

Human Cancer Cells ◽

Mouse Leukemia Virus ◽

Bovine Leukosis

By immunization of a rabbit with purified gs-antigen from mouse leukemia virus (MLV) a potent antiserum (R-gs-serum) was obtained, which reacts specifically with gs-antigen of MLV.In cat leukemia virus (KLV) two types of antigens, probably both group specific, could be demonstrated with anti-KLV-sera by Ouchterlony test. One of these was shown with MLV-sera to be identical with a MLV-gs-antigen component. This antigen occurring in both viruses is called gsinterspecies (interspec.) antigen.R-gs-serum allowed to detect gs-interspec. antigen also by the more sensitive CF-test. [Lispa (leukemia virus interspec. antigen) CF-test]. Preliminary experiments with this test indicated that bovine leukosis as well as human cancer cells can produce an agent in tissue culture which is serologically related to MLV.

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