scholarly journals Embryonic Tissue Culture of Laodelphax striatellus FALLÉN

1970 ◽  
Vol 14 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Kenichiro YAMADA ◽  
Takashi TOKUMITSU ◽  
Eishiro SHIKATA
Keyword(s):  
Tissue Culture ◽  
Embryonic Tissue ◽  
Laodelphax Striatellus

Viral Susceptibility and Embryonic Differentiation.

Development ◽  
1963 ◽  
Vol 11 (4) ◽  
pp. 757-764
Author(s):  
Juhani Rapola ◽  
Tapani Vainio ◽  
Lauri Saxén
Keyword(s):  
Tissue Culture ◽  
Viral Replication ◽  
Metabolic Rate ◽  
Tissue Damage ◽  
Embryonic Tissue ◽  
High Metabolic Rate ◽  
Viral Susceptibility ◽  
Proliferative Rate ◽  
Active Proliferation ◽  
Severe Tissue

The fact that viral susceptibility changes during embryogenesis has been pointed out by both experimental embryologists and clinical practitioners, not to mention virologists working with avian material. In attempts to find the fundamental factors which make embryonic tissue susceptible or resistant to a given virus, the metabolic and proliferative rate have been considered relevant (Williamson et al., 1953; Robertson et al., 1955; Töndury, 1956). Experience accumulated in studies of the replication of various viruses in tissue culture has taught us that a high metabolic rate and active proliferation may not always enhance viral replication (Ginsberg, 1958). However, there seems to be justification for the view that an injurious agent leads to more severe tissue damage when it exercises its effect upon actively proliferating tissues than when it does so at the ‘resting stage’.

scholarly journals Embryonic tissue culture of Inazuma dorsalis MOTSCHULSKY (Homoptera: Delphacidae)

1969 ◽  
Vol 13 (3) ◽  
pp. 159-161 ◽  
Author(s):  
Kenichiro YAMADA ◽  
Takashi TOKUMITSU ◽  
Eishiro SHIKATA
Keyword(s):  
Tissue Culture ◽  
Embryonic Tissue

Tissue section lifting for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 86-87
Author(s):  
Adrian F. van Dellen
Keyword(s):  
Infectious Disease ◽  
Electron Microscopy ◽  
Tissue Culture ◽  
Organ System ◽  
Surrounding Tissue ◽  
Paraffin Sections ◽  
Surface Areas ◽  
Specific Determination ◽  
High Degree ◽  
Accuracy And Repeatability

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

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