Characterization of Plant Epidermal Lens Effects by a Surface Replica Technique

Microvilli of HeLa cells cultured in vitro were preserved for electron microscopic examination at different stages of routine cultivation procedures. By a double-embedding technique, vertical sectioning for electron microscopy was possible. It revealed that, although the microvilli were present on all sides of the cell in the dispersed stage and in the attached stage, they were not present on the bottom of the cell when it was stretched on the surface of the dish. When the cells were grown in dense colonies, they were found on top of each other, and microvilli were present on all sides, except on the bottom surface of those cells in contact with the dish. We achieved a more dramatic demonstration of the microvilli by developing a surface-replica technique which retains their spatial arrangement and permits characterization of the distribution of their number, length, and diameter.

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Characterization of the Crack Initiation and Crack Propagation Behavior of Welded Steels by Means of the Replica Technique

Practical Metallography ◽

10.3139/147.110296 ◽

2014 ◽

Vol 51 (8) ◽

pp. 557-567

Author(s):

M. Panzenböck ◽

A. Horn ◽

G. Posch ◽

H. Clemens

Keyword(s):

Crack Initiation ◽

Crack Propagation ◽

Replica Technique ◽

Propagation Behavior ◽

Crack Propagation Behavior

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Applications of the skin surface replica technique in dermatology

Journal of Audiovisual Media in Medicine ◽

10.3109/17453058409155913 ◽

1984 ◽

Vol 7 (1) ◽

pp. 21-26 ◽

Cited By ~ 3

Author(s):

Jeremy R. Nayler

Keyword(s):

Skin Surface ◽

Replica Technique ◽

Surface Replica

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Fabrication and characterization of silver nanorods incorporated calcium silicate scaffold using polymeric sponge replica technique

Materials & Design ◽

10.1016/j.matdes.2020.109026 ◽

2020 ◽

Vol 195 ◽

pp. 109026 ◽

Cited By ~ 2

Author(s):

Pawan Kumar ◽

Brijnandan S. Dehiya ◽

Anil Sindhu ◽

Ravinder Kumar ◽

Catalin I. Pruncu ◽

...

Keyword(s):

Calcium Silicate ◽

Replica Technique ◽

Silver Nanorods ◽

Fabrication And Characterization

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Direct Imaging of Sodium Stearate Crystals Dispersed in Waterpropylene Glycol Mixtures by Cryo-Electron Microscopy

Microscopy and Microanalysis ◽

10.1017/s1431927600029743 ◽

2001 ◽

Vol 7 (S2) ◽

pp. 734-735

Author(s):

Yue Ma ◽

J. Liang ◽

Y. Zheng ◽

S. L. Erlandsen ◽

L. E. Scriven ◽

...

Keyword(s):

Electron Microscopy ◽

Practical Importance ◽

Sodium Stearate ◽

Original Structure ◽

Replica Technique ◽

X Ray Scattering ◽

Cryo Electron Microscopy ◽

Transmission Electron ◽

Surface Active

Cryo-scanning electron microscopy (cryo-SEM) and cryo-transmission electron microscopy (cryo- TEM), in conjunctions with rheological measurements, light and confocal microscopy, x-ray scattering, and solid state NMR, are used to characterize sodium stearate (NaSt) crystals dispersed in waterpropylene glycol (PG) mixtures at macroscopic, microscopic, molecular, and atomic levels. NaSt is a surface-active, structural agent in household and personal cleaning products, including deodorant sticks and soap bars. A better structural characterization of NaSt/PG/water systems has practical importance in personal care and cosmetic industries. NaSt crystals and other soap crystal morphologies have been studied by the TEM/replica technique. However, the replicas were made of the residue after the original sample or its aqueous dilution were dried, and the original structure may have been lost during drying. Cryo-SEM was not used to study NaSt crystals because of its lower resolution and because the crystals are highly susceptible to radiation damage by electron beam.

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Ultrastructural Studies Relating to the Surface Morphology of Cultured Cells

Journal of Cell Science ◽

10.1242/jcs.6.2.477 ◽

1970 ◽

Vol 6 (2) ◽

pp. 477-484

Author(s):

R. G. P. PUGH-HUMPHREYS ◽

W. SINCLAIR

Keyword(s):

Electron Microscopy ◽

Cultured Cells ◽

Kidney Cells ◽

Replica Technique ◽

Regular Feature ◽

Ultrastructural Studies ◽

Transmission Electron ◽

Surface Replica ◽

Mesenchyme Cells ◽

Canine Kidney

Scanning electron microscopy and a surface-replica technique for transmission electron microscopy have been used to study the ultrastructural features of cultured-cell surfaces The presence of microvilli measuring 0.1-0.2 µm in diameter by up to 5 µm in length has been noted as a regular feature of Landschütz ascites, ‘fibroblastic’ HeLa, and canine kidney, cells. The surfaces of chick mesenchyme cells were notably almost devoid of microvilli. The presence of microvilli at the cell surface is discussed briefly.

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Ultrastructural Surface Characterization of Cultured Intracranial Tumors

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091019 ◽

1976 ◽

Vol 34 ◽

pp. 206-207

Author(s):

T. Liszczak ◽

S. Jacobson ◽

P. Kornblith

Keyword(s):

Critical Point ◽

Surface Characterization ◽

Malignant Cell ◽

Intracranial Tumors ◽

Cell Surfaces ◽

Replica Technique

One property of a malignant cell is its decreased intercellular adhesiveness in vivo(l), and in vitro(2). In an attempt to visualize any morphological correlates associated with this phenomenon we utilized the replica technique to examine cell surfaces and cell to substrate attachment regions in cultured intracranial tumors, tumors from other sites and reactive cells.Cells were explanted onto glass coverslips in Leighton tubes, cultured to approximately 50%confluency, buffer rinsed, 2.5% glutaraldehyde fixed, chrome-osmium postfixed, critical point dried with Freon 13 and 113, and replicated with platinum carbon. The cells and coverslips were dissolved away by hydroflouric acid.

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