A surface replica technique for the examination of quartz sand grains in the electron microscope

1969 ◽  
Vol 39 (4) ◽  
pp. 1608-1610
Author(s):  
M. R. Hornby ◽  
W. B. Scott
Keyword(s):  
Electron Microscope ◽  
Quartz Sand ◽  
Replica Technique ◽  
Surface Replica

scholarly journals The influence of metahalloysite addition on tobermorite formation studied by X-ray powder diffraction and scanning electron microscope

2018 ◽  
Vol 163 ◽  
pp. 05008
Author(s):  
Anna Skawińska
Keyword(s):  
Phase Composition ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Hydrothermal Treatment ◽  
Powder Diffraction ◽  
Quartz Sand ◽  
Raw Material ◽  
Model Systems ◽  
X Ray ◽  
Scanning Electron

This paper presents the results of the studies carried out in the model systems and concerning the tobermorite synthesis with an addition of metahalloysite. Quartz sand and quicklime were the main raw material constituents. The mixtures in the form of slurries underwent hydrothermal treatment with an addition of metahalloysite (5%, 10%, 15%, 20% and 30%) for 4 hours and 12 hours. The resultant composites were analysed for their phase composition using X-ray powder diffraction. The microstructure was examined using the Scanning Electron Microscope. Tobermorite was the principle reaction product. When 30% metahalloysite was added to the mixture containing CaO and SiO2, the formation of katoite was found.

Characterization of Plant Epidermal Lens Effects by a Surface Replica Technique

1991 ◽  
Vol 42 (5) ◽  
pp. 581-587 ◽  
Author(s):  
GREG MARTIN ◽  
DAVID A. MYRES ◽  
THOMAS C. VOGELMANN
Keyword(s):  
Replica Technique ◽  
Surface Replica

Ultrastructural Studies Relating to the Surface Morphology of Cultured Cells

1970 ◽  
Vol 6 (2) ◽  
pp. 477-484
Author(s):  
R. G. P. PUGH-HUMPHREYS ◽  
W. SINCLAIR
Keyword(s):  
Electron Microscopy ◽  
Cultured Cells ◽  
Kidney Cells ◽  
Replica Technique ◽  
Regular Feature ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Surface Replica ◽  
Mesenchyme Cells ◽  
Canine Kidney

Scanning electron microscopy and a surface-replica technique for transmission electron microscopy have been used to study the ultrastructural features of cultured-cell surfaces The presence of microvilli measuring 0.1-0.2 µm in diameter by up to 5 µm in length has been noted as a regular feature of Landschütz ascites, ‘fibroblastic’ HeLa, and canine kidney, cells. The surfaces of chick mesenchyme cells were notably almost devoid of microvilli. The presence of microvilli at the cell surface is discussed briefly.

scholarly journals ELECTRON MICROSCOPE STUDIES OF THE MICROVILLI OF HELA CELLS

1967 ◽  
Vol 34 (2) ◽  
pp. 569-576 ◽  
Author(s):  
Harold W. Fisher ◽  
T. W. Cooper
Keyword(s):  
Hela Cells ◽  
Electron Microscopic Examination ◽  
Spatial Arrangement ◽  
Bottom Surface ◽  
Electron Microscopic ◽  
Replica Technique ◽  
Embedding Technique ◽  
Surface Replica

Microvilli of HeLa cells cultured in vitro were preserved for electron microscopic examination at different stages of routine cultivation procedures. By a double-embedding technique, vertical sectioning for electron microscopy was possible. It revealed that, although the microvilli were present on all sides of the cell in the dispersed stage and in the attached stage, they were not present on the bottom of the cell when it was stretched on the surface of the dish. When the cells were grown in dense colonies, they were found on top of each other, and microvilli were present on all sides, except on the bottom surface of those cells in contact with the dish. We achieved a more dramatic demonstration of the microvilli by developing a surface-replica technique which retains their spatial arrangement and permits characterization of the distribution of their number, length, and diameter.

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