scholarly journals Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation

2005 ◽  
Vol 57 (1) ◽  
pp. 201-212 ◽  
Author(s):  
Paul E. Verslues ◽  
Elizabeth A. Bray
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Water Potential ◽  
Proline Accumulation ◽  
Low Water Potential ◽  
Aba Insensitive

scholarly journals Natural variation identifies genes affecting drought-induced abscisic acid accumulation in Arabidopsis thaliana

2017 ◽  
Vol 114 (43) ◽  
pp. 11536-11541 ◽  
Author(s):  
Rajesh Kalladan ◽  
Jesse R. Lasky ◽  
Trent Z. Chang ◽  
Sandeep Sharma ◽  
Thomas E. Juenger ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Water Potential ◽  
Natural Variation ◽  
Lipid Binding ◽  
Reverse Genetic ◽  
Loss Of Function ◽  
Start Domain ◽  
Domain Protein ◽  
Low Water Potential

Accumulation of the stress hormone abscisic acid (ABA) in response to drought and low water-potential controls many downstream acclimation mechanisms. However, mechanisms controlling ABA accumulation itself are less known. There was a 10-fold range of variation in ABA levels among nearly 300 Arabidopsis thaliana accessions exposed to the same low water-potential severity. Genome-wide association analysis (GWAS) identified genomic regions containing clusters of ABA-associated SNPs. Candidate genes within these regions included few genes with known stress or ABA-related function. The GWAS data were used to guide reverse genetic analysis, which found effectors of ABA accumulation. These included plasma-membrane–localized signaling proteins such as receptor-like kinases, aspartic protease, a putative lipid-binding START domain protein, and other membrane proteins of unknown function as well as a RING U-box protein and possible effect of tonoplast transport on ABA accumulation. Putative loss-of-function polymorphisms within the START domain protein were associated with climate factors at accession sites of origin, indicating its potential involvement in drought adaptation. Overall, using ABA accumulation as a basis for a combined GWAS–reverse genetic strategy revealed the broad natural variation in low-water-potential–induced ABA accumulation and was successful in identifying genes that affect ABA levels and may act in upstream drought-related sensing and signaling mechanisms. ABA effector loci were identified even when each one was of incremental effect, consistent with control of ABA accumulation being distributed among the many branches of ABA metabolism or mediated by genes with partially redundant function.

Involvement of Abscisic Acid in Controlling Plant Growth in Soil of Low Water Potential

1993 ◽  
Vol 20 (5) ◽  
pp. 425 ◽  
Author(s):  
R Munns ◽  
RE Sharp
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Water Potential ◽  
Cell Expansion ◽  
Shoot Growth ◽  
Xylem Sap ◽  
Leaf Expansion ◽  
Root And Shoot Growth ◽  
Dry Soil

Hormones appear to be important in controlling plant growth in soils of low water potential, particularly in changing the root:shoot ratio as the soil dries or becomes saline, and in communicating soil conditions to the leaves. This review has necessarily focused on abscisic acid (ABA), as there is little information about the role of other hormones in controlling growth in dry or saline soils. ABA is partly responsible for the differential response of root and shoot growth to dry soils. In dry soil it maintains root growth and inhibits shoot growth. However, when applied to well-watered plants, it usually inhibits root and shoot growth, showing that plants in dry soil respond quite differently from well-watered plants. ABA affects the rate of cell expansion in plants in dry soils: it maintains cell expansion in roots and inhibits that in leaves. It may also affect the rate of cell production, but little is known about this. The role of ABA as a long-distance signal in controlling growth by root-to-shoot communication is unclear: the concentrations found in xylem sap can affect stomatal conductance, but seem too low to affect leaf expansion. Yet drought and salinity generally affect leaf expansion before they affect leaf conductance. A possible solution to this puzzle is that ABA is transported in xylem sap in a complexed form, or that another compound in xylem sap stimulates the synthesis or activity of ABA in leaves, or affects leaf expansion independently of ABA.

scholarly journals Alvaradoa amorphoides germination at low water potential and the role of the antioxidant system

2015 ◽  
Vol 93 (2) ◽  
Author(s):  
Verónica Hernández-Pérez ◽  
Judith Márquez Guzmán ◽  
Sobeida Sánchez-Nieto ◽  
Rocio Cruz-Ortega
Keyword(s):  
Water Potential ◽  
Antioxidant System ◽  
La Selva ◽  
Low Water Potential ◽  
Superóxido Dismutasa

<p><em>Alvaradoa amorphoides</em> es un árbol representativo de la Selva Baja Caducifolia de la zona de Xochicalco en el Estado <br />de Morelos, México. En este trabajo se evaluaron los requerimientos hídricos para la germinación de esta especie, tanto en campo como en laboratorio. En campo se evaluó la germinación en sitios con diferente geomorfología y características de humedad. En laboratorio se evaluó el porcentaje de germinación, imbibición, respiración y actividad de enzimas antioxidantes en semillas colocadas en soluciones de diferente potencial hídrico. El porcentaje de germinación en campo fue de 42%, pero fue diferente entre sitios independientemente de la humedad relativa y de la geomorfología del suelo. Las semillas expuestas a -0.5 MPa retrasaron su germinación, extendiendo la fase II de la germinación. Los potenciales de -1.0 y -1.5 MPa inhibieron la germinación 80 y 100%, respectivamente; sin embargo, las semillas permanecieron viables. El consumo de oxígeno no varió en las semillas expuestas a los diferentes potenciales hídricos, aunque sí su perfil respiratorio, ya que no mostraron las tres fases características. Los niveles de H<sub>2</sub>O<sub>2</sub> y O<sub>2</sub>-, y la actividad de catalasa, superóxido dismutasa y glutatión reductasa no fueron significativamente diferentes entre los potenciales hídricos probados (-0.5 y -1.0 MPa), solo la actividad de la ascorbato peroxidasa fue inhibida. Los resultados muestran que las semillas de <em>A. amorphoides</em> permanecen en fase II de la germinación en potenciales hídricos negativos, sin presentar niveles altos de especies reactivas de oxígeno (ERO) y permaneciendo viables, lo que podría explicar por qué el porcentaje de germinación no se vio afectado por la geoforma y la humedad en condiciones de campo.</p>

Applicability of abscisic acid and (or) proline accumulation as selection criteria for drought tolerance in Nicotiana tabacum

1994 ◽  
Vol 72 (10) ◽  
pp. 1535-1540 ◽  
Author(s):  
L. van Rensburg ◽  
G. H. J. Krüger
Keyword(s):  
Abscisic Acid ◽  
Drought Tolerance ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Proline Accumulation ◽  
Leaf Water ◽  
Cell Wall Elasticity ◽  
Drought Tolerant ◽  
Acid Accumulation ◽  
Selection Parameters

The efficacy of various aspects of abscisic acid and proline accumulation as potential selection parameters for drought tolerance in tobacco was evaluated under controlled conditions. The results indicated that both abscisic acid (though being less pronounced) and proline accumulate rapidly after a distinct threshold leaf water potential value has been reached and that probably because of their higher cell wall elasticity (0.23 and 0.28 MPa for the drought-tolerant cultivars GS46 and Elsoma, respectively, compared with 0.39 and 0.31 MPa for the drought-sensitive cultivars TL33 and CDL28, respectively) these threshold leaf water potential values are reached sooner in drought-tolerant cultivars. However, abscisic acid accumulation precedes proline accumulation in both the drought-tolerant and drought-sensitive cultivars. Proline concentrations increased sharply at a leaf water potential of ca. −1.27 MPa in the drought-tolerant cultivars and at a leaf water potential of ca. −1.50 MPa in the drought-sensitive cultivars. At a leaf water potential of −0.77 MPa the abscisic acid concentrations of all four cultivars were already significantly higher than those of their respective controls and were greater in the drought-tolerant cultivars. The leaf water potential value at which abscisic acid and proline start accumulating rapidly and the accumulated proline end concentrations are recommended as selection parameters for drought tolerance in tobacco. Key words: abscisic acid accumulation, cell-wall elasticity, drought stress, Nicotiana tabacum L., proline accumulation, selection parameters.

scholarly journals Role of Abscisic Acid in the Induction of Desiccation Tolerance in Developing Seeds of Arabidopsis thaliana

1992 ◽  
Vol 98 (4) ◽  
pp. 1484-1493 ◽  
Author(s):  
Cor Meurs ◽  
Amarjit S. Basra ◽  
Cees M. Karssen ◽  
Leendert C. van Loon
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Desiccation Tolerance ◽  
Developing Seeds

Abscisic acid influences the susceptibility of Arabidopsis thaliana to Pseudomonas syringae pv. tomato and Peronospora parasitica

2003 ◽  
Vol 30 (4) ◽  
pp. 461 ◽  
Author(s):  
Peter G. Mohr ◽  
David M. Cahill
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Pseudomonas Syringae ◽  
Bacterial Pathogen ◽  
Peronospora Parasitica ◽  
Aba Signal Transduction ◽  
Pathogen Challenge ◽  
Aba Insensitive ◽  
Avirulent Isolate ◽  
Increased Susceptibility

The phytohormone abscisic acid (ABA) plays a major role in the regulation of many physiological stresses although its role in pathogen-induced stress remains poorly understood. We examined the influence of ABA on interactions of Arabidopsis thaliana (L.) Heynh. (Arabidopsis) with a bacterial pathogen, Pseudomonas syringae pv. tomato and an Oomycete, Peronospora parasitica. Both addition of 100 μM ABA to plants and drought stress stimulated increased susceptibility of Arabidopsis to an avirulent isolate of P. syringae pv. tomato. In contrast, an ABA-deficient mutant of Arabidopsis, aba1-1, displayed reduced susceptibility to virulent isolates of P. parasitica. An ABA-insensitive mutant, abi1-1, that is impaired in ABA signal transduction did not alter in susceptibility to either P. syringae pv. tomato or P. parasitica. These results demonstrate that the concentration of endogenous ABA at the time of pathogen challenge is important for the development of susceptibility in Arabidopsis.

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