First report and molecular prevalence of potential zoonotic Enterocytozoon bieneusi in Turkish tumbler pigeons (Columba livia domestica)

2021 ◽  
Author(s):  
Didem Pekmezci ◽  
Gamze Yetismis ◽  
Zeynep Nurselin Colak ◽  
Onder Duzlu ◽  
Gamze Nur Ozkilic ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Potential Role ◽  
Columba Livia ◽  
Enterocytozoon Bieneusi ◽  
Human Infection ◽  
Opportunistic Pathogens ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
Microsporidian Species

Abstract A total of 250 droppings of tumbler pigeons (Columba livia domestica, Columbidae) were collected individually from different breeders in Turkey, to investigate the presence and genotyping of microsporidian species by nested PCR and to reveal their zoonotic potential. In the present study, Enterocytozoon bieneusi was the only microsporidian species identified in 35 pigeons with an overall molecular prevalence of 14.0%. Only one known genotype zoonotic Peru6 was identified in all positive samples according to the sequence analyses of the internal transcribed spacer region of ribosomal DNA of E. bieneusi. This study represents the first report of E. bieneusi in pigeons in Turkey. Our study also confirms the competence of breeding pigeons as hosts for the zoonotic Peru6 genotype, corroborating its potential role as a source of human infection and environmental contamination. Lay summary Microsporidia are spore-producing fungi defined as emerging opportunistic pathogens of humans. The occurrence of microsporidia in animals could be risky for human public health. Home kept breeding pigeons pose a high risk for transmission of the microsporidians to humans.

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

scholarly journals Mammalian Microsporidiosis

2000 ◽  
Vol 37 (2) ◽  
pp. 113-128 ◽  
Author(s):  
K. Wasson ◽  
R. L. Peper
Keyword(s):  
Animal Species ◽  
Comparative Anatomy ◽  
Polar Filament ◽  
Enterocytozoon Bieneusi ◽  
Human Infection ◽  
Opportunistic Pathogens ◽  
Encephalitozoon Cuniculi ◽  
Obligate Intracellular ◽  
Encephalitozoon Intestinalis ◽  
Potential Pathogen

The phylum Microspora contains a diverse group of single-celled, obligate intracellular protozoa sharing a unique organelle, the polar filament, and parasitizing a wide variety of invertebrate and vertebrate animals, including insects, fish, birds, and mammals. Encephalitozoon cuniculi is the classic microsporidial parasite of mammals, and encephalitozoonosis in rabbits and rodents has been and continues to be recognized as a confounding variable in animal-based biomedical research. Although contemporary research colonies are screened for infection with this parasite, E. cuniculi remains a cause of morbidity and mortality in pet and conventionally raised rabbits. In addition, E. cuniculi is a potential pathogen of immature domestic dogs and farm-raised foxes. The recent discovery and identification of Encephalitozoon intestinalis, Encephalitozoon hellem, and Enterocytozoon bieneusi, in addition to E. cuniculi, as opportunistic pathogens of humans have renewed interest in the Microspora. Veterinary pathologists, trained in the comparative anatomy of multiple animal species and infectious disease processes, are in a unique position to contribute to the diagnosis and knowledge of the pathogenesis of these parasitic diseases. This review article covers the life cycle, ultrastructure, and biology of mammalian microsporaidia and the clinical disease and lesions seen in laboratory and domestic animals, particularly as they relate to Encephalitozoon species. Human microsporidial disease and animal models of human infection are also addressed. Often thought of as rabbit pathogens of historical importance, E. cuniculi and the related mammalian microsporidia are emerging as significant opportunistic pathogens of immunocompromised individuals.

Choiromyces cerebriformis (Tuberaceae), a new hypogeous species from Yunnan, China

Phytotaxa ◽  
2021 ◽  
Vol 482 (3) ◽  
pp. 251-260
Author(s):  
TIAN-JUN YUAN ◽  
OLIVIER RASPÉ ◽  
YONG-JUN LI ◽  
LI WANG ◽  
KAI-MEI SU ◽  
...  
Keyword(s):  
Related Species ◽  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Genetic Distances ◽  
Morphological Evidence ◽  
Similar Species ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
Morphological Differences ◽  
Light Orange

A new hypogeous species, Choiromyces cerebriformis sp. nov. is described and illustrated from Yunnan province, China. Both morphological evidence and sequence analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) support the species as new to science. C. cerebriformis differs from other Choiromyces species in having ascomata with larger lobes and light orange-brown gleba, and globose ascospores with short stick-like sparse spines ornamentation. Morphological differences and genetic distances with the similar species C. helanshanensis and C. alveolatus are discussed. A phenotypic key including related species is provided.

First Report of Macrophomina phaseolina Causing Leaf and Stem Blight of Tropical Soda Apple in Florida

2007 ◽  
Vol 8 (1) ◽  
pp. 40 ◽  
Author(s):  
Fanny Iriarte ◽  
Erin Rosskopf ◽  
Mark Hilf ◽  
Greg McCollum ◽  
Joe Albano ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Macrophomina Phaseolina ◽  
Vegetable Crops ◽  
Stem Tissue ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
Stem Blight ◽  
Leaf Necrosis ◽  
Tropical Soda Apple ◽  
Noxious Weed

Leaf necrosis was observed in tropical soda apple plants in Fort Pierce, FL. A fungus was isolated from symptomatic stem tissue and from numerous excised pycnidia. Identity was confirmed by analysis of the sequence of the internal transcribed spacer region, and Koch's postulates were completed. To the best of our knowledge this is the first report of M. phaseolina as a pathogen of S. viarum. This report provides further evidence of this noxious weed serving as a reservoir for potential pathogens of vegetable crops. Accepted for publication 25 September 2007. Published 15 November 2007.

scholarly journals Amanita pseudovaginata from Pakistan

2020 ◽  
Vol 5 (3) ◽  
pp. 19
Author(s):  
Abdul Nasir Khalid ◽  
Arooj Naseer
Keyword(s):  
Molecular Analysis ◽  
Fruiting Body ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Its Sequences ◽  
Published Data ◽  
Fungal Symbiont ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
Quercus Spp

Amanita pseudovaginata of Amanita subgenus Amanita sect. Vaginatae was found associated with Quercus spp. forests during a survey of macrofungi from oaks forests of Pakistan. The fruiting body was characterized morphoanatomically as well as by molecular analysis. The identification of the fungal symbiont as Amanita pseudovaginata was confirmed by Internal Transcribed Spacer Region (ITS) sequences. Sporocarps were matched with published data available from Russia and China. Phylogenetic analyses and morphological descriptions are provided. This represents the first report of this species in Pakistan.

scholarly journals Molecular Detection and Genotyping of Intestinal Microsporid-ia from Stray Dogs in Iran

2019 ◽  
Author(s):  
Moein DELROBAEI ◽  
Shahram JAMSHIDI ◽  
Parviz SHAYAN ◽  
Elahe EBRAHIMZADE ◽  
Iraj ASHRAFI TAMAI ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Capital City ◽  
Urine Samples ◽  
Internal Transcribed Spacer Region ◽  
Stray Dogs ◽  
Transmission Potential ◽  
Time Period ◽  
Specific Fragment

Background: Microsporidia as one of the most important pathogens in veterinary and agricultural settings, have emerged in immunocompromised patients in Iran. To date, different Enterocytozoon bieneusi genotypes have been identified in humans and animals, supporting the possibility of zoonotic zoonosis transmission potential. The aim of this study was to evaluate the distribution of E. bieneusi genotypes among overpopulated stray dogs in vicinity of Tehran, the capital city of Iran. Methods: Totally, 75 stool and 75 urine samples were obtained from 75 stray dogs during the time period from Mar 2015 to Oct 2015. DNA extraction was performed on all the samples and specific fragment of small subunit ribosomal RNA gene of E. bieneusi and Encephalitozoon spp. was amplified. Furthermore, specific primers targeting the internal transcribed spacer region of E. bieneusi were applied to determine the genotype of the microorganism. Results: Microsporidia was detected in 5.3% of stool samples, while none of the urine samples was positive for microsporidia species. Overall, 440 bp fragment of E. bieneusi was amplified in all the samples and there was no amplification for Encephalitozoon spp. The results of sequencing of 410 bp fragment of internal transcribed spacer region showed that all the E. bieneusi were genotype D. Conclusion: E. bieneusi was the most prevalent microsporidian species in the stray dogs and all the positive isolates were characterized as genotype D.

Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade

2004 ◽  
Vol 50 (12) ◽  
pp. 1001-1006 ◽  
Author(s):  
Wladyslav I Golubev ◽  
José P Sampaio ◽  
Leonor Alves ◽  
Nikita W Golubev
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Novel Species ◽  
Biosphere Reserve ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
26S Rdna ◽  
Yeast Strains ◽  
Rdna Sequencing ◽  
Steppe Plants

Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains and strain CBS 8016 have almost identical sequences and belong to the Holtermannia clade of the Tremellomycetidae (Basidiomycota, Hymenomycetes). A novel species named Cryptococcus festucosus (type strain VKM Y-2930) is proposed to accommodate these strains. Physiological characteristics and mycocin sensitivity patterns distinguishing Cryptococcus festucosus from the other species of this clade are presented.Key words: mycocin sensitivity, rDNA sequencing, Cryptococcus, Holtermannia.

Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F1 hybrids of Tecoma (Bignoniaceae)

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 437-445 ◽  
Author(s):  
Ryan N. Contreras ◽  
John M. Ruter ◽  
Joann Conner ◽  
Yajuan Zeng ◽  
Peggy Ozias-Akins
Keyword(s):  
Internal Transcribed Spacer ◽  
Potential Role ◽  
F1 Hybrids ◽  
Internal Transcribed Spacer Region ◽  
Pistillate Parent ◽  
Fertile Hybrids ◽  
Species Hybrids ◽  
Rdna Copy

Interspecific hybridization in Tecoma Juss. was conducted to develop novel forms for the nursery industry. We report fertile hybrids from the cross T. garrocha Hieron. (pistillate parent) × T. stans (L.) Juss. ex Kunth. Leaf morphology of the F1 hybrids of T. garrocha × T. stans was intermediate between the parents. GISH also confirmed hybridity. The F1 hybrids were successfully backcrossed to both parents and self-pollinated to produce BC and F2 progeny. Tecoma garrocha , T. stans, and T. guarume A. DC. ‘Tangelo’ were self-fertile. The F1 hybrids also were crossed with T. capensis (Thunb.) Lindl. and T. guarume ‘Tangelo’, resulting in three-species hybrids. FISH conducted on F1 hybrids identified four copies of the 18S internal transcribed spacer region. Further analysis using FISH has the potential to provide information on the evolution of Bignoniaceae and the potential role of polyploidy.

scholarly journals First report of Fusarium proliferatum causing stem and root rot on lucky bamboo (Dracaena braunii) in Iraq

2019 ◽  
Vol 12 (1) ◽  
pp. 1-5
Author(s):  
A.A. Lahuf
Keyword(s):  
Ribosomal Dna ◽  
Root Rot ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Its Region ◽  
Fusarium Proliferatum ◽  
Ornamental Plant ◽  
Sequence Analyses ◽  
First Report

Summary Lucky bamboo (Dracaena braunii) is a popular ornamental plant in Iraq. Individuals of this plant showing stem and root rot symptoms were observed during a survey conducted from November 2015 to February 2016 in several nurseries in Kerbala province, Iraq. Based on morphological characteristics and sequence analyses of the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA), the pathogen was identified as Fusarium proliferatum. This is the first report of stem and root rot caused by F. proliferatum on lucky bamboo (D. braunii) in Iraq.

scholarly journals Diversity and Aggressiveness of Rhizoctonia solani and Rhizoctonia-like Fungi on Vegetables in New York

Plant Disease ◽  
2009 ◽  
Vol 93 (6) ◽  
pp. 615-624 ◽  
Author(s):  
Mana Ohkura ◽  
George S. Abawi ◽  
Christine D. Smart ◽  
Kathie T. Hodge
Keyword(s):  
New York ◽  
Sequence Analysis ◽  
Rhizoctonia Solani ◽  
Internal Transcribed Spacer ◽  
Anastomosis Group ◽  
Internal Transcribed Spacer Region ◽  
Snap Bean ◽  
First Report ◽  
Pathogenicity Tests ◽  
Waitea Circinata

Vegetable growers in New York, especially those growing table beets, have recently observed that the corn rotation is no longer effective in suppressing diseases caused by Rhizoctonia solani and Rhizoctonia-like fungi. To investigate this problem, 68 isolates of Rhizoctonia solani and Rhizoctonia-like fungi infecting vegetables in New York were isolated, characterized, and their pathogenicity on corn determined. Sequence analysis of the rDNA internal transcribed spacer region inferred 26 isolates to belong to R. solani anastomosis group (AG) 2-2 and 19 isolates to belong to AG 4. Remaining isolates belonged to AG 1, AG 2-1, AG 5, AG 11, Ceratobasidium AG (CAG) 2, CAG 6, and Waitea circinata var. zeae. This is a first report of AG 11 and W. circinata var. zeae recovered from naturally infected vegetables in New York. Pathogenicity tests on corn showed that the majority of isolates are pathogenic on corn, and isolates belonging to AG 2-2, AG 5, and AG 11 exhibited high aggressiveness. These results suggest that certain strains of R. solani and Rhizoctonia-like fungi infecting vegetables in New York have acquired the ability to infect corn. In addition, snap bean was inoculated with seven isolates exhibiting low to high aggressiveness on corn, and a correlation between aggressiveness on corn and snap bean was observed.

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