Frequency of Streptococcus mutants in patients having dental caries; a data from clinical setting in rural area of Balochistan

To assess the frequency of streptococcus mutans (S. mutans) and its association with socio-demographic factors in patients having dental caries presenting at a DHQ hospital in rural area of Balochistan. It was a cross-sectional study conducted at the clinical setting or DHQ hospital of a rural area in Balochistan from April 2020 January 2021. About 120 patients of age 18 to 40 years of either gender presenting dental caries were included in the study using non-random consecutive sampling technique. Data regarding age, gender, ethnicity, addictions (like gutka and smoking) pH level and oral hygiene was obtained from all the participants by researcher himself. The number of S. mutans colonies (CFU) was counted using a self-illuminating binocular microscope after plates were incubated for 48 hours at 37°C. All findings were entered into a pre-designed proforma. SPSS version 23 was used to analyze data. The median age of patients with dental caries was 28 years (IQR: 21-35 years) and 83.3% of the patients were males. The median S. mutans count of all the patients with dental caries was estimated as 35 with IQR as 32 to 38. Mann-Whitney statistics showed significant difference between ethnic groups for S. mutans count (p=0.049). A weak correlation was observed between S. Mutans count and pH level (r=0.206) with statistically insignificant difference (p=0.206). Frequency of S. mutans is 100% in individuals with dental caries. S. mutan counts have a significant association with ethnicity and weak correlation with pH in saliva.

Complexity analysis of algorithms: A case study on bioinformatics tools

The data volume produced by the omic sciences nowadays was driven by the adoption of new generation sequencing platforms, popularly called NGS (Next Generation Sequencing). Among the analysis performed with this data, we can mention: mapping, genome assembly, genome annotation, pangenomic analysis, quality control, redundancy removal, among others. When it comes to redundancy removal analysis, it is worth noting the existence of several tools that perform this task, with proven accuracy through their scientific publications, but they lack criteria related to algorithmic complexity. Thus, this work aims to perform an algorithmic complexity analysis in computational tools for removing redundancy of raw reads from the DNA sequencing process, through empirical analysis. The analysis was performed with sixteen raw reads datasets. The datasets were processed with the following tools: MarDRe, NGSReadsTreatment, ParDRe, FastUniq, and BioSeqZip, and analyzed using the R statistical platform, through the GuessCompx package. The results demonstrate that the BioSeqZip and ParDRe tools present less complexity in this analysis

Callus induction and chemical characterization of cell suspension cultures of jojoba (Simmondsia chinensis L.)

Jojoba (Simmondsia chinensis L.) oil is also known as liquid wax or fixed oil. It is an important metabolite of jojoba having commercial importance in cosmetics as well as a potential biofuel source. We presented an efficient system for in vitro establishment of cell suspension cultures (CSC) from proliferating friable calluses. For this purpose, cotyledon, internode, and leaf explants were cultured on MS medium + 1, 2, 4, 6, 8 or 10 µM 2, 4-Dichlorophenoxyacetic acid (2, 4-D), α-Naphthalene acetic acid (NAA) alone or in combination with 1 or 2 µM N6-benzylaminopurine (BAP) or Kinetin. Results demonstrated that 100% healthy, friable and variegated calluses were obtained on 8 µM, 10 µM 2, 4-D or 2, 4-D 10 µM + 2 µM BAP and represented as callus lines (CL) CL-1, CL-2 or CL-3, respectively, after 38 days. One-gram callus tissue per CL was then immersed in the respective liquid medium and agitated on an orbital shaker at 60-70 rpm under the growth room conditions (25 ± 2 °C, 16 h light period) for the preparation of CSC. After 15 days, CSC was sieved and large clumps were removed. Growth measurement of CSC was determined by cell counting, packed cell volume (PCV) and cell viability. The highest number of viable cells was obtained at 2.57 OD with CL-3, where PCV was highest (0.35 ml) on CL-1 of 38 days old calluses. 2,3,5-Triphenyltetrazolium chloride was a reliable approach for the determination of cell viability of CSC.

Invitro mycoparasitism activity of Trichoderma spp against Fusarium solani inciting root rot of chickpea ( Cicer arietinum L.)

Root rot of chickpea (Cicer arietinum L.) is caused by Fusarium solani. This paper describes the efficacy of Trichoderma spp. against sensitive and resistant isolates of F, solani by dual culture method under invitro conditions. Trichoderma virens, T. atroviride, T. viride, T. harzianum, T. koningiopsis, T. stilbohypoxyli,and T. pseudokoningii species were used for the antagonistic study. Results indicate that all Trichoderma species showed great antagonistic activity. But among them, T. virens, T. atroviride, T. viride showed 90% and 80 % antagonistic activity than others in case of a sensitive isolate of test fungus. The resistant isolate of the pathogen was restricting the antagonism to some extent.

Salinity stress resistance in wheat

Wheat is used as staple food worldwide and it ranked third in cereals. Its productivity a the global level decreases by many stresses mainly by salinity stress which is associated with different physiological and biochemical processes of plants. To overcome these growth and yield reduction issues, salinity resistance in wheat can be achieved. The introduction of resistance to salinity-induced water stress and ion toxicity in wheat lead to more reliable results. Salt tolerance mechanisms at tissues and whole plant levels along with sequestration of toxic ions can improve overall growth, yield, and salinity resistance capability in wheat. Different sources and measurements of salinity play important role in the production of salinity tolerant wheat. This article mainly reviews different physiological mechanisms, genetics, omics, and quality trait loci approaches for the production of salinity tolerant wheat.

Antimicrobial potential of eucalyptus oil and lemongrass oil alone and in combination against clinical bacteria in planktonic and biofilm mode

The use of natural substances has been trending from past few years. In recent years, the synthetic products obtained from plants have gained immense importance. Essential oils and volatile products obtained from plants are the source of food flavoring, aroma products and fragrance industries. Their use is also common to cure different ailments such as cancer, skin problems and nosocomial infections. Vast research has shown the antimicrobial properties of essential oils obtained from plants. Essential oils of lemongrass and eucalyptus have antiviral, antibacterial, antioxidant and insecticidal characteristics. Lemongrass and eucalyptus oils shows their antibacterial activity towards Gram positive bacteria such as Staphylococcus aureus, Bacillus subtilis and Gram-negative bacteria such as Escherichia coli and Klebsiella pneumonia. Combination of these oils also depicts high antimicrobial activity against different strains of bacteria. This review highlights the use of essential oils of lemongrass and eucalyptus for their in vitro antimicrobial properties against different bacteria in planktonic and biofilm mode. Mode of action by which the oil shows its inhibitory activity will be discussed. Susceptibility shown by different bacteria towards these essential oils and their components will be described

Genetic variability and association study of yield attributing traits in F2 populations of American cotton

Cotton is one of the most important fiber crops, grown all over the world. Genetic variability is a crucial factor from evolutionary point of view for crop species. It defines the adaptation of populations by allowing them to alter their genetic constitution in changing environment. Genetic variability in Gossypium hirsutum is declining due to selection pressure, hence causing low yield. Present study was conducted to evaluate ten parental genotypes with their F2 generations to uncover their genetic potential for yield by accessing genetic variability, heritability, genetic advance and association between fiber, yield and yield linked traits. High values of phenotypic and genotypic coefficient of variance were observed for node of first fruiting branch, monopodia per plant, boll weight, fiber strength and yield per plant. All morphological and fiber quality traits represented broad sense heritability ranging from 51% to 90%. High value of genetic advance was shown by plant height and yield per plant. Positive association of yield per plant was recorded for traits such as plant height, node of first fruiting branch, monopodia per plant, number of bolls per plant, seeds per boll, and ginning out turn. Additionally, F2 progeny of CIM-534×MNH-814 had high yield per plant along with maximum number of bolls per plant and seed per boll. So, such F2 progeny can further be explored to improve yield and yield contributing traits.

Characterization and in-vivo toxicological evaluation of imidacloprid nanoemulsion in rats

Acute pesticide poisoning is an important public health problem worldwide and accounts for a significant number of deaths occurring each year. The present article aimed to investigate toxic effects of imidacloprid (IMD) nanoemulsion formulated using ultrasound dispersion technique, and characterized using FTIR, TEM anddynamic light scattering in adult rats. The synthesized Nano-emulsion droplets are mainly spherical in shape and their sizes ranged between (19nm –128 nm) with zeta potential of −38.8± 0.mV. Also, The median lethal dose (LD50) of nano imidacloprid in rats was 39 mg/kg body weight. Administration of different doses of 3, 1.4 and 0.8 mg.kg mg/kg b.wt. of IMD Nano emulsion to rats for 21 days, adversely affects the body weight and weight gain, and resulted in a significant increase in serum serum ALT, AST activities , glucose, Creatinine, urea and cholesterol concentrations, as well as reduced serum total proteins, Albumin and globulin as compared to control rats. The results clearly suggest that treatment with IMD nanoemulsion adversely affects the liver & kidney functions which confirmed by the histopathological findings. Nanoemulsion form and also increases the DNA damage as confirmed by the comet test

Honey bees show dance pattern to communicate – A review

The honey bee language is considered by many to be one of the most interesting systems for animal communications, used for recruitment to food sources. Honeybee's forager dancers communicate food and other resources to the household by quantity, consistency, direction, and spatial location. The waggle dance was interesting and complex, which bees used for spatial information on desired resources. All honeybee species use the waggle dance to convey their position and distance from food sources and possible new nest sites. The research was carried out on dance communication, earlier ideas, controversies, and solutions gave a broad overview. In this analysis, unique problems are focused on as follows: (a) multiple dance forms. (b) Distance and path calculation (c) How bees do dark hive dance.? Several experiments verified that bees perform various kinds of dance, depending on their particular task. There is, however, still a lack of comprehensive knowledge on other types of dances, which help us solve numerous questions and help us better understand the meaning of the different kinds of dances carried in and outside the hive by honeybees.

Influence of defoliation during reproductive stage on seed yield in uniculm mungbean plants

An experiment was conducted at the pot yard of Bangladesh Institute of Nuclear Agriculture, Mymensingh during the period from February to May 2018 to investigate the effect of defoliations on morphological characters, yield attributes and yield of uniculm mungbean plant. The experiment comprised of nine levels of defoliation viz., control, 2, 3, 4, 5 leaves removal from base and 2, 3, 4 and 5 leaves removal from top out of 7 leaves during flowering start phase. The morpho-physiological characters such as plant height, stem diameter, leaf area and total dry mass plant-1 and number of racemes, reproductive characters such as rachis length and diameter, number of flowers plant-1 and reproductive efficiency, yield contributing characters such as number of pods plant-1, pod length, number of seeds pod-1, single pod and 100-seed weight and seed yield decreased with increasing defoliation both from base and top except basal 2 leaves defoliation. The above parameters increased over control in basal 2 leaves defoliation. The decrement due to defoliation was greater in top defoliated plants than the corresponding basal defoliated ones indicating upper leaves contributes more assimilate to the sink than the basal leaves. The highest morpho-physiological parameters, reproductive characters and yield contributing characters observed in basal 2 leaves defoliated plants which resulted the highest seed yield (5.75 g plant-1) followed by control (5.49 g plant-1). The lowest morpho-physiological parameters, reproductive and yield contributing characters recorded in top 5 defoliated plants and produced the lowest seed yield (1.21 g plant-1). These results suggest that uniculm mungbean plant tolerate basal 2 leaves loss in the canopy during reproductive stage.