Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint

Abstract We have identified an S-phase DNA damage checkpoint in Schizosaccharomyces pombe. This checkpoint is dependent on Rad3, the S. pombe homolog of the mammalian ATM/ATR checkpoint proteins, and Cds1. Cds1 had previously been believed to be involved only in the replication checkpoint. The requirement of Cds1 in the DNA damage checkpoint suggests that Cds1 may be a general target of S-phase checkpoints. Unlike other checkpoints, the S. pombe S-phase DNA damage checkpoint discriminates between different types of damage. UV-irradiation, which causes base modification that can be repaired during G1 and S-phase, invokes the checkpoint, while γ-irradiation, which causes double-stranded breaks that cannot be repaired by a haploid cell if induced before replication, does not invoke the checkpoint. Because the same genes are required to respond to UV- and γ-irradiation during G2, this discrimination may represent an active suppression of the γ response during S-phase.

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Cell cycle G2 arrest induced by HIV-1 Vpr in fission yeast (Schizosaccharomyces pombe) is independent of cell death and early genes in the DNA damage checkpoint

Virus Research ◽

10.1016/s0168-1702(00)00167-2 ◽

2000 ◽

Vol 68 (2) ◽

pp. 161-173 ◽

Cited By ~ 30

Author(s):

Robert T. Elder ◽

Min Yu ◽

Mingzhong Chen ◽

Steven Edelson ◽

Yuqi Zhao

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Death ◽

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Dna Damage Checkpoint ◽

G2 Arrest ◽

Early Genes ◽

Hiv 1

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Isolation and Characterization of mas1+of Schizosaccharomyces pombe, a Homologue of Human CIP29/Hcc-1 Involved in the Regulation of Cell Division

Journal of Life Science ◽

10.5352/jls.2011.21.12.1666 ◽

2011 ◽

Vol 21 (12) ◽

pp. 1666-1677

Author(s):

Jae-Young Cha ◽

Sang-Min Shin ◽

Se-Eun Ha ◽

Jung-Sup Lee ◽

Jong-Kun Park

Keyword(s):

Cell Division ◽

Schizosaccharomyces Pombe ◽

Isolation And Characterization

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Isolation and characterization of Schizosaccharomyces pombe mutants with defective NAD-dependent malic enzyme

Canadian Journal of Microbiology ◽

10.1139/m86-088 ◽

1986 ◽

Vol 32 (6) ◽

pp. 481-486 ◽

Cited By ~ 13

Author(s):

C. Osothsilp ◽

R. E. Subden

Keyword(s):

Schizosaccharomyces Pombe ◽

Malic Enzyme ◽

Pool Analysis ◽

Starch Gel Electrophoresis ◽

Wild Type ◽

Isolation And Characterization ◽

Starch Gel ◽

Colony Color ◽

Color Indicator

To obtain NAD-dependent malic enzyme mutants of Schizosaccharomyces pombe, a colony color indicator screening system was developed. Mutants defective for malic acid utilization (mau mutants) are yellow, while wild-type colonies are blue on the defined bromcresol green based indicator medium. NAD-dependent malic enzyme mutants were distinguished from other mau mutants by subsequent, starch gel electrophoresis, spectrophotometry, complementation tests, and intermediate pool analysis with cell-free extracts.

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Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

Molecular and Cellular Biology ◽

10.1128/mcb.14.6.3895-3905.1994 ◽

1994 ◽

Vol 14 (6) ◽

pp. 3895-3905

Author(s):

S Kjaerulff ◽

J Davey ◽

O Nielsen

Keyword(s):

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Mutational Analysis ◽

M Cells ◽

Gene Products ◽

Structural Genes ◽

Triple Mutant ◽

Isolation And Characterization ◽

Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

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