scholarly journals Primer design for a prokaryotic differential display RT-PCR

1997 ◽  
Vol 25 (9) ◽  
pp. 1830-1835 ◽  
Author(s):  
R. Fislage ◽  
M. Berceanu ◽  
Y. Humboldt ◽  
M. Wendt ◽  
H. Oberender
Keyword(s):  
Differential Display ◽  
Primer Design ◽  
Rt Pcr

Primer design and transcript quantification of a highly multiplexed RT‐PCR for a nonmodel avian species

2011 ◽  
Vol 12 (1) ◽  
pp. 116-122 ◽  
Author(s):  
SUSAN L. BALENGER ◽  
CHRISTOPHER J. W. McCLURE ◽  
GEOFFREY E. HILL
Keyword(s):  
Primer Design ◽  
Avian Species ◽  
Rt Pcr ◽  
Transcript Quantification

scholarly journals Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes

1999 ◽  
Vol 32 (7) ◽  
pp. 845-852 ◽  
Author(s):  
A.M. Silva ◽  
E.G. Pires ◽  
E.F. Abrantes ◽  
L.R.P. Ferreira ◽  
R.T. Gazzinelli ◽  
...  
Keyword(s):  
Differential Display ◽  
Rt Pcr

Identification of Genes Induced by Taxol Application Using a Combination of Differential Display RT-PCR and DNA Microarray Analysis

2001 ◽  
Vol 56 (9-10) ◽  
pp. 814-819 ◽  
Author(s):  
Ei-ichiro Fukusaki ◽  
Takashi Oishi ◽  
Hozumi Tanaka ◽  
Shin-ichiro Kajiyama ◽  
Akio Kobayashi
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Differential Display ◽  
Gene Expression Pattern ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Dna Microarray Analysis ◽  
Glass Slides ◽  
Polymerase Chain ◽  
Cdna Fragments

Abstract The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.

Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

scholarly journals From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

2015 ◽  
Vol 23 (2) ◽  
Author(s):  
Adrian Man ◽  
Claudia Bănescu ◽  
Minodora Dobreanu ◽  
Cornel Fraefel
Keyword(s):  
Nucleic Acid ◽  
Molecular Biology ◽  
Molecular Cloning ◽  
Primer Design ◽  
Free Software ◽  
Good Knowledge ◽  
Rt Pcr ◽  
Pcr Assays ◽  
Validation Of Results

AbstractSuccessful experiments in molecular biology require good knowledge about various methods and protocols. In molecular biology, nucleic acid manipulation is the essence, starting with the quality of extraction and ending with several analysis assays (PCR, RT-PCR, qPCR, PCR arrays, molecular cloning, etc). Though many of these are so called “standardized”, in practice there are many variables that can influence the outcome of the experiment. Due to the importance of optimal primer design in PCR assays, we will focus on primer designing and checking software, but we also present other useful free tools that can help researchers in the molecular biology field

180 Inventory of “atherosclerotic genes” induced or repressed in endothelial cells and smooth muscle cells by differential display RT-PCR

1997 ◽  
Vol 11 ◽  
pp. 51
Author(s):  
Anton J.G. Horrevoets ◽  
Carlie J.M. de Vries ◽  
A.E. van Achterberg ◽  
Ruud D. Fontijn ◽  
Benien van Aken ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Differential Display ◽  
Muscle Cells ◽  
Rt Pcr

Molecular cloning of oxygen-inducible genes in Caenorhabditis elegans by RT-PCR differential display

1996 ◽  
Vol 3 (2) ◽  
pp. 107-110 ◽  
Author(s):  
Yoshitaka Kushibiki ◽  
Naoaki Ishii ◽  
Sumino Yanase ◽  
Hiroe Nakazawa
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Cloning ◽  
Differential Display ◽  
Rt Pcr ◽  
Inducible Genes
Sign in / Sign up

Export Citation Format

Share Document