scholarly journals Characterization of a novel mouse cDNA, ES18, involved in apoptotic cell death of T-cells

1999 ◽  
Vol 27 (6) ◽  
pp. 1524-1530 ◽  
Author(s):  
E. Park
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Mouse Cdna

scholarly journals Flow Cytometry and FTIR Spectroscopy for Detection of Early Apoptosis in Human T Cells

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1558 ◽  
Author(s):  
Günnur Güler ◽  
Ayten Nalbant
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Cell Death ◽  
Ftir Spectroscopy ◽  
Apoptotic Cell ◽  
Annexin V ◽  
Apoptotic Cell Death ◽  
Biochemical Changes ◽  
Human T Cells ◽  
Early Apoptosis

Apoptosis, a programmed cell death, has a vital role in various cellular processes. Apoptotic cells exhibit morphological and biochemical changes, detected by a variety of assays (caspases, mitochondrial dyes, DNA laddering). Flow cytometry is a powerful tool for detection of apoptotic cell death and allows information about the cell size and molecules associated with cell-bound antibodies. Recently, Fourier transform infrared (FTIR) spectroscopy as rapid and low-cost tool has been extensively used for cellular studies, providing information on cellular structures. The aim of this study was to detect early apoptosis and obtain further insights into the capability of FTIR spectroscopy, comparing the results with flow cytometry. In this study, apoptotic cell death was induced in human Jurkat T cells with Camptothecin (CPT), a DNA topoisomerase I inhibitor. Cells were cultured with 4µM CPT in RPMI (with 5% FCS) for 24 h. Immunoflourescence labeling for multicolor flow cytometry was accomplished with Annexin V concomitantly with 7-AAD. The same cells were also analyzed with ATR-FTIR spectroscopy. Flow cytometry data represents that the cells are Annexin V positive but 7AAD negative. This indicates that cells are in the early apoptotic stage, only externalization of phosphatidylserine exists on the plasma membrane. FTIR data reveals that membrane phospholipids and proteins undergo changes; fatty acid acyl chains are disordered and increased in mobility after treatment, which result from the early apoptosis process after CPT-treatment, confirmed by the flow cytometry. A combined study of flow cytometry and FTIR spectroscopy for analysis of apoptosis in human T cells exhibited compatible and complementary results. Existence of biophysical and biochemical changes in T cells after treatment were also demonstrated.

Functional Significance of Adhesion Molecules in Fas-Dependent Apoptotic Cell Death Induced by Interleukin-2-Activated T Cells

1998 ◽  
Vol 27 (4-5) ◽  
pp. 309-322 ◽  
Author(s):  
Yan-Wen Zhou ◽  
Yoshihiro Komada ◽  
Hiroto Inaba ◽  
Takao Deguchi ◽  
Kenji Sugiyama ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Adhesion Molecules ◽  
Functional Significance ◽  
Apoptotic Cell ◽  
Interleukin 2 ◽  
Apoptotic Cell Death ◽  
Activated T Cells

Immunological unresponsiveness and apoptotic cell death of T cells in measles virus infection

1995 ◽  
Vol 37 (3) ◽  
pp. 308-314 ◽  
Author(s):  
MICHAEL MARK ADDAE ◽  
YOSHIHIRO KOMADA ◽  
XAO-LI ZHANG ◽  
MINORU SAKURAI
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Virus Infection ◽  
Measles Virus ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

scholarly journals Doxycycline is an NF-κB inhibitor that induces apoptotic cell death in malignant T-cells

Oncotarget ◽  
2016 ◽  
Vol 7 (46) ◽  
pp. 75954-75967 ◽  
Author(s):  
Carolina V. Alexander-Savino ◽  
Matthew S. Hayden ◽  
Christopher Richardson ◽  
Jiyong Zhao ◽  
Brian Poligone
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Malignant T Cells

scholarly journals Upregulated TNFR Family Member Insufficient to Promote Apoptotic Cell Death in T Helper 17 Cells

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1526
Author(s):  
Tufan Utku Çalışkan ◽  
Ayten Nalbant
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Cd4 T Cells ◽  
Th17 Cells ◽  
Apoptotic Cell ◽  
B Cell Lymphoma ◽  
Apoptotic Cell Death ◽  
T Helper ◽  
T Helper 17 ◽  
T Helper 17 Cells

Fas is the receptor of tumor necrosis family receptors (TNFR) and involves in apoptosis. Since discovery of T helper 17 cells (Th17) in 2005, which are defined as a new type of helper T cells, it has become clear that the dysregulated function Th17 cells and their cytokines could contribute to pathology of diseases including autoimmune diseases and cancer. There is not much known about apoptotic and survival mechanisms of Th17 cells in the literature. Therefore, the players of apoptotic cell death in Th17 cells were investigated in the study. To carry out designed experiments, venous blood were drawn from the healthy volunteers with approval from the Noninvasive Ethics Committee. Peripheral blood mononuclear cells (PBMCs) were isolated from blood with Ficoll separation method. The naïve CD4+ T cells were sorted from the PBMC. Sorted naive T cells were cultured under Th17 polarizing conditions. The activation, differentiation and apoptosis related molecules of cultured cells were monitored by Flow cytometry. Data showed that naive CD4+ T cells were activated and differentiated into Th17 cells. Activated Th17 cells were Fas positive. Activated, Fas positive Th17 cells did not underwent significant plasma membrane changes. Furthermore, it was also observed that there was not much change in the Bcl-2 protein level. Bcl-2 protein is belongs to B-cell-lymphoma-2 (Bcl-2) family proteins and is major regulator of intrinsic apoptotic pathway as promoting cell survival. In addition to that the expression of Bclx-L, is an anti-apoptotic protein, were increased in these cells. Data indicates that Th17 cells (under Th17 polarization condition) were increased expression of anti-apoptotic Bcl-2 family members.

scholarly journals Interleukin-10 rescues T cells from apoptotic cell death: association with an upregulation of Bcl-2

Immunology ◽  
1997 ◽  
Vol 92 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S. B. A. COHEN ◽  
J. B. CRAWLEY ◽  
M. C. KAHAN ◽  
M. FELDMANN ◽  
B. M. J. FOXWELL
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Interleukin 10 ◽  
Apoptotic Cell Death

scholarly journals Apoptotic cell death of primed CD45RO+ T lymphocytes in Epstein-Barr virus-induced infectious mononucleosis

Blood ◽  
1992 ◽  
Vol 80 (2) ◽  
pp. 452-458 ◽  
Author(s):  
T Uehara ◽  
T Miyawaki ◽  
K Ohta ◽  
Y Tamaru ◽  
T Yokoi ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Central Feature ◽  
Activated T Cells ◽  
Barr Virus ◽  
Epstein Barr

The expansion of activated T cells, characterized by the expression of CD45RO as well as HLA-DR antigens, is a central feature in acute infectious mononucleosis (IM) induced by primary infection of Epstein- Barr virus (EBV). However, the fate of these activated T cells in this disease is not clearly understood. We found that, on simple culture, a large proportion of T cells isolated from acute IM patients died rapidly, but only a few T cells from normal individuals did. Morphologic observations and DNA fragmentation analysis showed that the loss of viability of IM T cells after incubation was mediated by apoptosis. IM T cells undergoing apoptosis resided exclusively in the CD45RO+ populations of both CD4+ and CD8+ T cells, most of which were shown to coexpress apoptosis-related Fas antigen. Some cytokines such as interleukin-2 (IL-2), IL-5, and IL-6 could rescue IM T cells from apoptotic cell death. The results seemed to imply that most of primed (CD45RO+) T cells in acute IM might be subject to apoptotic cell death, possibly when leaving from the local sites actively producing certain soluble factors required for their survival. Our studies suggest the programmed cell death of peripheral mature T cells as a mechanism of antigen-driven selection.

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