scholarly journals A case of bacterial keratitis caused by multi-drug-resistant Shewanella algae without marine exposure

2021 ◽  
Vol 2021 (11-12) ◽  
Author(s):  
Je Eun Song ◽  
Sollip Kim ◽  
Hyung Koo Kang ◽  
Inkwon Chung ◽  
Yee Gyung Kwak ◽  
...  
Keyword(s):  
Vegetative State ◽  
Bacterial Keratitis ◽  
Drug Resistant ◽  
Ionization Time ◽  
Gram Negative ◽  
Flight Mass Spectrometry ◽  
Shewanella Algae ◽  
Rrna Sequencing ◽  
Human Infections ◽  
Suspected Pneumonia

ABSTRACT Shewanella are Gram-negative rods and marine pathogens. Here, we report a case of bacterial keratitis caused by Shewanella algae without marine exposure. A 66-year-old man with suspected pneumonia was sent to the emergency department from a nursing hospital. He had been in there for 2 years in a vegetative state and could not close his eyes voluntarily. Neither the patient nor his family had experienced any marine exposure. Keratitis was suspected in his right eye. Gram-negative rods grew from swab culture and identified as S. algae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA sequencing. The patient was treated with topical tobramycin, moxifloxacin and ofloxacin as well as steroids for 14 days, and the keratitis improved. S. algae is a rare human pathogen, and most human infections involve marine exposure. This is the second report of bacterial keratitis caused by S. algae worldwide and the first in Asia.

scholarly journals Severe Wound Infection withPhotobacterium damselaessp.damselaeandVibrio harveyi, following a Laceration Injury in Marine Environment: A Case Report and Review of the Literature

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jörg Hundenborn ◽  
Steffi Thurig ◽  
Mechthild Kommerell ◽  
Heike Haag ◽  
Oliver Nolte
Keyword(s):  
Wound Infection ◽  
Data Retrieval ◽  
Marine Microorganisms ◽  
Sequencing Data ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Rdna Sequencing ◽  
Photobacterium Damselae ◽  
High Level ◽  
Human Infections

Marine microorganisms are uncommon etiologies of skin and skin structure infections, that is, wound infections. We report a case of severe wound infection, caused by the marinePhotobacterium damselae(Vibrionaceae), in a 64-year-old male patient, returning from Australia. The isolate tested positive for pPHDD1, a plasmid conferring high-level virulence. Furthermore, the wound was coinfected withVibrio harveyi, a halophile bacterium, which has never been reported from human infections before. Identification was achieved by use of Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry (MALDI-TOF) and confirmed by 16S rDNA sequencing. Data retrieval from bibliography was complicated sinceP. damselaehas been renamed often with a number of synonyms present in the literature:Photobacterium damsela,Vibrio damselae,Vibrio damsela,Pasteurella damselae, andListonella damsela. With all synonyms used as query terms, a literature search provided less than 20 cases published worldwide. A majority of those cases presenting as severe wound infection are even fatal following progression into necrotizing fasciitis. Management with daily wound dressing and antibiotic therapy (ofloxacin empirically, followed by doxycycline after availability of microbiology) led in the reported case to a favorable outcome, which seems to be, however, the exception based on a review of the available literature.

scholarly journals Comparing Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group

2015 ◽  
Vol 53 (11) ◽  
pp. 3580-3588 ◽  
Author(s):  
Raquel Arinto-Garcia ◽  
Marcos Daniel Pinho ◽  
João André Carriço ◽  
José Melo-Cristino ◽  
Mário Ramirez
Keyword(s):  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Streptococcus Anginosus ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Subspecies Level ◽  
Human Infections ◽  
Tof Ms

The heterogeneity of members of theStreptococcus anginosusgroup (SAG) has traditionally hampered their correct identification. Recently, the group was subdivided into 6 taxa whose prevalence among human infections is poorly described. We evaluated the accuracy of the Rapid ID32 Strep test, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and a PCR multiplex method to identify 212 SAG isolates recovered from human infections to the species and subspecies level by using multilocus sequence analysis (MLSA) as the gold standard. We also determined the antimicrobial susceptibilities of the isolates. Representatives of all SAG taxa were found among our collection. MALDI-TOF MS and the Rapid ID32 Strep test correctly identified 92% and 68% of the isolates to the species level, respectively, but showed poor performance at the subspecies level, and the latter was responsible for major identification errors. The multiplex PCR method results were in complete agreement with the MLSA identifications but failed to distinguish the subspeciesStreptococcus constellatussubsp.pharyngisandS. constellatussubsp.viborgensis. A total of 145 MLSA sequence types were present in our collection, indicating that within each taxon a number of different lineages are capable of causing infection. Significant antibiotic resistance was observed only to tetracycline, erythromycin, and clindamycin and was present in most taxa. MALDI-TOF MS is a reliable method for routine SAG species identification, while the need for identification to the subspecies level is not clearly established.

scholarly journals Prosthetic valve endocarditis caused by Pasteurella in a penicillin allergic patient: challenges in diagnosis and treatment

2012 ◽  
Vol 4 (1) ◽  
pp. 32 ◽  
Author(s):  
Giovanni Satta ◽  
Rebecca Louise Gorton ◽  
Hala Kandil
Keyword(s):  
Pasteurella Multocida ◽  
Prosthetic Valve Endocarditis ◽  
Laser Desorption ◽  
Allergic Patient ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Rrna Sequencing ◽  
Microbiological Techniques ◽  
Immunocompromised State

<em>Pasteurella multocida</em> is a rare cause of infective endocarditis with only a few cases described. This report involves a 38-year-old penicillin-allergic patient in an immunocompromised state with several co-morbidities. Two molecular microbiological techniques, 16S rRNA sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were used to confirm the species identification as <em>P. multocida</em>. Previous reports in the literature are also reviewed.

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