Nanostructured probes to enhance optical and vibrational spectroscopic imaging for biomedical applications

2017 ◽  
Author(s):  
Anil K. Kodali ◽  
Rohit Bhargava
Keyword(s):  
Raman Spectroscopy ◽  
Biomedical Applications ◽  
Spectroscopic Imaging ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Optical Resonances ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman ◽  
Ir And Raman

This article describes the use of nanostructured probes to enhance optical and vibrational spectroscopic imaging for biomedical applications. Engineered probes and surfaces are promising tools for enhancing signals for ultrasensitive detection of diseases like carcinoma. Two methods of interest are surface-enhanced infrared absorption (SEIRA) spectroscopy and surface-enhanced Raman spectroscopy (SERS) for IR and Raman modalities, respectively. SERS and SEIRA can be broadly categorized under a common modality termed surface-enhanced vibrational spectroscopy. This article first reviews various breakthrough findings reported in SERS and SEIRA, along with different types ofsubstrates and contrast agents used in realizing the enhancement and theories proposed to explain these findings. It then considers the configurations of nano-LAMPs and presents example results demonstrating their optical resonances and tunability. Finally, it evaluates a few techniques for fabricating multilayered nanoparticles and highlights some issues with respect to fabrication.

scholarly journals Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Xiaowei Cao ◽  
Zhenyu Wang ◽  
Liyan Bi ◽  
Jie Zheng
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cell Lung Cancer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Small Cell ◽  
Label Free ◽  
Small Cell Lung ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a good candidate for the development of fast and easy-to-use diagnostic tools, possibly used on serum in screening tests. In this study, a potential label-free serum test based on SERS spectroscopy was developed to analyze human serum for the diagnosis of the non-small cell lung cancer (NSCLC). We firstly synthesized novel highly branched gold nanoparticles (HGNPs) at high yield through a one-step reduction of HAuCl4 with dopamine hydrochloride at 60°C. Then, HGNP substrates with good reproducibility, uniformity, and high SERS effect were fabricated by the electrostatically assisted (3-aminopropyl) triethoxysilane-(APTES-) functionalized silicon wafer surface-sedimentary self-assembly method. Using as-prepared HGNP substrates as a high-performance sensing platform, SERS spectral data of serum obtained from healthy subjects, lung adenocarcinoma patients, lung squamous carcinoma patients, and large cell lung cancer patients were collected. The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. SERS spectra were analyzed by principal component analysis (PCA), which was able to distinguish different types of NSCLC. Considering its time efficiency, being label-free, and sensitivity, SERS based on HGNP substrates is very promising for mass screening NSCLC and plays an important role in the detection and prevention of other diseases.

Surface Modification of Gold Nanostars Through the Changes on Gold Seed Concentrations

MRS Advances ◽  
2018 ◽  
Vol 3 (41) ◽  
pp. 2449-2454
Author(s):  
Juan Carlos Martinez Espinosa ◽  
Miguel Jose Yacaman ◽  
German Plascencia Villa ◽  
Victor Hugo Romero Arellano ◽  
Ana Karen Zavala Raya
Keyword(s):  
Biomedical Applications ◽  
Surface Enhanced Raman Spectroscopy ◽  
Diameter Distribution ◽  
Biological Assays ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman ◽  
Gold Seed ◽  
Gold Nanomaterials ◽  
Specific Membrane

AbstractDue to its excellent optical properties, gold nanomaterials with anisotropic morphology are playing an important role in biomedical applications, specifically in the use of Surface Enhanced Raman Spectroscopy (SERS) technique for biological assays. In this work, we verified the behavior of the star shape nanoparticle peaks obtained by chemical synthesis (precursor reactant: HAuCl4, cationic surfactant: CTAB) and whose peaks were formed from the different concentrations of gold seeds (55, 65, 75 and 85 ul) which were added to the total solution (5.275 ml). The shape and size of the nanoparticles was verified with a Hitachi S-5500 microscope with a BF & DF SEM / STEM detector, and for the diameter distribution (hydrodynamic) was carried out by the dynamic light distribution technique with a Malvern DLS system Zetasizer Nano ZS. Particle sizes (peak-to-peak considering) were obtained with variations from 107 to 166 nm. The results suggest adding 75 ul of gold seeds to obtain uniform nanostars with well defined peaks. These gold nano-stars could be applied for identification of specific membrane markers for the study of different types of cancer by the SERS technique.

Ultrasensitive detection of phenolic antioxidants by surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Nancy Ornelas-Soto ◽  
Iris A. Aguilar-Hernández ◽  
Nils Kristian Afseth ◽  
Tzarara López-Luke ◽  
Flavio Contreras-Torres ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Phenolic Antioxidants ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface enhanced Raman spectroscopy of Aurora kinases: direct, ultrasensitive detection of autophosphorylation

RSC Advances ◽  
2013 ◽  
Vol 3 (13) ◽  
pp. 4221 ◽  
Author(s):  
Soumik Siddhanta ◽  
Dhanasekaran Karthigeyan ◽  
Partha P. Kundu ◽  
Tapas K. Kundu ◽  
Chandrabhas Narayana
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Aurora Kinases ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

ZnO–Ag hybrids for ultrasensitive detection of trinitrotoluene by surface-enhanced Raman spectroscopy

2014 ◽  
Vol 16 (28) ◽  
pp. 14706-14712 ◽  
Author(s):  
Xuan He ◽  
Hui Wang ◽  
Zhongbo Li ◽  
Dong Chen ◽  
Qi Zhang
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Hybrid Substrates ◽  
Tnt Detection

Low-cost SERS sensors were fabricated by 4-ATP-functionalized ZnO–Ag hybrid substrates for TNT detection with high sensitivity, selectivity and reproducibility.

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