A light microscopical morphometric study of the luminal epithelium of human endometrium during the peri-implantation period

Abstract The conceptuses (embryo/fetus and placental membranes) of pigs require energy to support elongation and implantation, and amounts of glucose and fructose increase in the uterine lumen during the peri-implantation period. Conceptuses from day 16 of pregnancy were incubated with either 14C-glucose or 14C-fructose and amounts of radiolabeled CO2 released from the conceptuses measured to determine rates of oxidation of glucose and fructose. Glucose and fructose both transport into conceptuses, and glucose is preferentially metabolized in the presence of fructose, whereas fructose is actively metabolized in the absence of glucose and to a lesser extent in the presence of glucose. Endometrial and placental expression of glucose transporters SLC2A1, SLC2A2, SCL2A3, and SLC2A4 were determined. SLC2A1 messenger RNA (mRNA) and protein, and SLC2A4 mRNA were abundant in the uterine luminal epithelium of pregnant compared to cycling gilts, and increased in response to progesterone and conceptus-secreted estrogen. SLC2A2 mRNA was expressed weakly by conceptus trophectoderm on day 15 of pregnancy, whereas SLC2A3 mRNA was abundant in trophectoderm/chorion throughout pregnancy. Therefore, glucose can be transported into the uterine lumen by SLC2A1, and then into conceptuses by SLC2A3. On day 60 of gestation, the cell-specific expression of these transporters was more complex, suggesting that glucose and fructose transporters are precisely regulated in a spatial-temporal pattern along the uterine-placental interface of pigs to maximize hexose sugar transport to the pig conceptus/placenta.

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Role and Regulation of the Serum- and Glucocorticoid-Regulated Kinase 1 in Fertile and Infertile Human Endometrium

Endocrinology ◽

10.1210/en.2007-0659 ◽

2007 ◽

Vol 148 (10) ◽

pp. 5020-5029 ◽

Cited By ~ 43

Author(s):

Fakhera Feroze-Zaidi ◽

Luca Fusi ◽

Masashi Takano ◽

Jenny Higham ◽

Madhuri S. Salker ◽

...

Keyword(s):

Marker Gene ◽

Human Endometrium ◽

Pcr Analysis ◽

Luminal Epithelium ◽

Uterine Receptivity ◽

Forkhead Transcription Factor ◽

Endometrial Stromal Cells ◽

Stromal Compartment ◽

Infertile Women

Using cDNA microarray analysis, we identified SGK1 (serum- and glucocorticoid-regulated kinase 1) as a gene aberrantly expressed in midsecretory endometrium of women with unexplained infertility. SGK1 is a serine/threonine kinase involved primarily in epithelial ion transport and cell survival responses. Real-time quantitative PCR analysis of a larger, independent sample set timed to coincide with the period of uterine receptivity confirmed increased expression of SGK1 transcripts in infertile women compared with fertile controls. We further demonstrate that SGK1 expression is regulated by progesterone in human endometrium in vivo as well as in explant cultures. During the midsecretory phase of the cycle, SGK1 mRNA and protein were predominantly but not exclusively expressed in the luminal epithelium, and expression in this cellular compartment was higher in infertile women. In the stromal compartment, SGK1 expression was largely confined to decidualizing cells adjacent to the luminal epithelium. In primary culture, SGK1 was induced and phosphorylated upon decidualization of endometrial stromal cells in response to 8-bromo-cAMP and progestin treatment. Moreover, overexpression of SGK1 in decidualizing cells enhanced phosphorylation and cytoplasmic translocation of the forkhead transcription factor FOXO1 and inhibited the expression of PRL, a major decidual marker gene. Conversely, knockdown of endogenous SGK1 by small interfering RNA increased nuclear FOXO1 levels and enhanced PRL expression. The observation that SGK1 targets FOXO1 in differentiating human endometrium, together with its distinct temporal and spatial expression pattern and increased expression in infertile patients, suggest a major role for this kinase in early pregnancy events.

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408 Morphometric study of endometrial luminal epithelium in electromagnetic field exposed rats

Toxicology Letters ◽

10.1016/s0378-4274(03)90407-3 ◽

2003 ◽

Vol 144 ◽

pp. s111

Author(s):

Leila Rowshangar ◽

Rad J. Soleimani

Keyword(s):

Electromagnetic Field ◽

Morphometric Study ◽

Luminal Epithelium

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Morphometric study of the human endometrium following continuous exposure to levonorgestrel released from vaginal rings during 90 days

Contraception ◽

10.1016/0010-7824(91)90074-p ◽

1991 ◽

Vol 43 (4) ◽

pp. 361-374 ◽

Cited By ~ 11

Author(s):

E. Johannisson ◽

I. Brosens ◽

F. Cornillie ◽

M. Elder ◽

J. White ◽

...

Keyword(s):

Human Endometrium ◽

Morphometric Study ◽

Continuous Exposure ◽

Vaginal Rings

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410 Steroids Regulate SLC2A1 and SLC2A3 to Deliver Glucose into Trophectoderm for Metabolism via Glycolysis

Journal of Animal Science ◽

10.1093/jas/skaa278.348 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 188-189

Author(s):

Gregory A Johnson ◽

Avery C Kramer ◽

Chelsie Steinhauser ◽

Heewon Seo ◽

Bryan A McLendon ◽

...

Keyword(s):

Temporal Pattern ◽

Sugar Transport ◽

Glucose Transporters ◽

Luminal Epithelium ◽

Specific Expression ◽

Uterine Lumen ◽

Placental Membranes ◽

Oxidation Of Glucose ◽

Implantation Period

Abstract The conceptuses (embryo/fetus and placental membranes) of pigs require energy to support elongation and implantation, and amounts of glucose and fructose increase in the uterine lumen during the peri-implantation period. Conceptuses from Day 16 of pregnancy were incubated with either 14C-glucose or 14C-fructose and amounts of radiolabeled CO2 released from the conceptuses measured to determine rates of oxidation of glucose and fructose. Both glucose and fructose transport into conceptuses, and glucose is preferentially metabolized in the presence of fructose, while fructose is actively metabolized in the absence of glucose and to a lesser extent in the presence of glucose. Endometrial and placental expression of glucose transporters SLC2A1, SLC2A2, SCL2A3 and SLC2A4 were determined. SLC2A1 mRNA and protein, and SLC2A4 mRNA were abundant in the uterine luminal epithelium of pregnant compared to cycling gilts, and increased in response to progesterone and conceptus-secreted estrogen. SLC2A2 mRNA was expressed weakly by conceptus trophectoderm on Day 15 of pregnancy, while SLC2A3 mRNA was abundant in trophectoderm/chorion throughout pregnancy. Therefore, glucose can be transported into the uterine lumen by SLC2A1, and then into conceptuses by SLC2A3. On Day 60 of gestation, the cell-specific expression of these transporters was more complex, suggesting that glucose and fructose transporters are precisely regulated in a spatial-temporal pattern along the uterine-placental interface of pigs to maximize hexose sugar transport to the pig conceptus/placenta.

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Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Reproduction ◽

10.1530/rep.1.00861 ◽

2006 ◽

Vol 131 (1) ◽

pp. 139-151 ◽

Cited By ~ 24

Author(s):

Jing Cong ◽

Hong-Lu Diao ◽

Yue-Chao Zhao ◽

Hua Ni ◽

Yun-Qin Yan ◽

...

Keyword(s):

Implantation Site ◽

Prostaglandin E ◽

Luminal Epithelium ◽

Prostaglandin I2 ◽

Rat Uterus ◽

Pregnant Rats ◽

Cox 2 ◽

Prostacyclin Synthase ◽

Implantation Period ◽

Cox 1

It has been shown that both prostaglandin I2 (PGI2) and PGE2 are essential for mouse implantation, whereas only PGE2 is required for hamster implantation. To date, the expression and regulation of cyclooxygenase (COX) and prostaglandin E synthase (PGES), which are responsible for PGE2 production, have not been reported in the rat. The aim of this study was to examine the expression pattern and regulation of COX-1, COX-2, membrane-associated PGES-1 (mPGES-1), mPGES-2 and cytosolic PGES (cPGES) in rat uterus during early pregnancy and pseudopregnancy, and under delayed implantation. At implantation site on day 6 of pregnancy, COX-1 immunostaining was highly visible in the luminal epithelium, and COX-2 immunostaining was clearly observed in the subluminal stroma. Both mPGES-1 mRNA and protein were only observed in the subluminal stroma surrounding the implanting blastocyst at the implantation site on day 6 of pregancy , but were not seen in the inter-implantation site on day 6 of pregnancy and on day 6 of pseudopregnancy. Our data suggest that the presence of an active blastocyst is required for mPGES-1 expression at the implantation site. When pregnant rats on day 5 were treated with nimesulide for 24 h, mPGES-1 protein expression was completely inhibited. cPGES immunostaining was clearly observed in the luminal epithelium and subluminal stromal cells immediately surrounding the implanting blastocyst on day 6 of pregnancy. mPGES-2 immunostaining was clearly seen in the luminal epithelium at the implantation site. Additionally, immunostaining for prostaglandin I synthase (PGIS) was also strongly detected at the implantation site. In conclusion, our results indicate that PGE2 and PGI2 should have a very important role in rat implantation.

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