Polysomal RNA's of Rat Liver: Identification and Characterization of Several Kinds of High Molecular Weight RNA Other than 18S and 28S Species

1971 ◽  
Vol 70 (3) ◽  
pp. 451-455 ◽  
Author(s):  
Masamichi TAKAGI ◽  
Tadashi INOUE ◽  
Yasukiyo UMEMURA
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Rat Liver ◽  
Identification And Characterization

Identification and characterization of a polysaccharide-containing antigen fromSchistosoma mansonieggs which cross-reacts with the surface of schistosomula

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 255-268 ◽  
Author(s):  
D. W. Dunne ◽  
Q. D. Bickle
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
High Molecular Weight ◽  
Concanavalin A ◽  
Trichloroacetic Acid ◽  
Soluble Fraction ◽  
Cell Adherence ◽  
Dependent Cell ◽  
Identification And Characterization

SUMMARYAntisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction ofSchistosoma mansoniSEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to byS. mansoni-infected mouse sera, and has been designated K3(Kappa 3). The rabbit antisera were used to partially characterize antigen K3as havingMrin the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3serum also reacted with the surface ofS. bovis, S. haematobiumand to a lesser extentS. japonicumschistosomula.

scholarly journals Identification and Characterization of High-Molecular-Weight Glutenin Subunits from Agropyron intermedium

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e87477 ◽  
Author(s):  
Shuanghe Cao ◽  
Zhixin Li ◽  
Caiyan Gong ◽  
Hong Xu ◽  
Ran Yang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Glutenin Subunits ◽  
Agropyron Intermedium ◽  
Identification And Characterization
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