scholarly journals Engineering Fusarium Head Blight Resistance in Wheat by Expression of a Fusion Protein Containing a Fusarium-Specific Antibody and an Antifungal Peptide

2008 ◽  
Vol 21 (9) ◽  
pp. 1242-1248 ◽  
Author(s):  
He-Ping Li ◽  
Jing-Bo Zhang ◽  
Run-Ping Shi ◽  
Tao Huang ◽  
Rainer Fischer ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Fusarium Head Blight ◽  
Recombinant Antibody ◽  
Crop Protection ◽  
Fusarium Head Blight Resistance ◽  
Natural Resistance ◽  
Antifungal Peptide ◽  
Type I ◽  
Head Blight ◽  
Antibody Fusion Protein

Fusarium head blight (FHB) or scab of wheat is a devastating disease in warm and humid regions at wheat-flowering periods worldwide. Natural resistance against FHB pathogens is inadequate and the development of FHB-resistant wheat cultivars has been a challenge. Expression of pathogen-specific antibodies in plants has been proposed as a strategy for crop protection. In this study, an antibody fusion protein comprising a Fusarium-specific recombinant antibody derived from chicken and an antifungal peptide from Aspergillus giganteus was expressed in wheat as a method for protecting plants against FHB pathogens. Plants expressing the antibody fusion displayed a very significantly enhanced resistance in T2 and T3 generations upon single-floret inoculation with the macroconidia of Fusarium asiaticum, the predominant species causing FHB in China, indicating a type II resistance. Spraying inoculation further revealed an enhanced type I resistance in the transgenic wheat plants. Remarkably, more grains were produced in the transgenic plants than the nontransgenic controls. Our results demonstrated that the antibody fusion protein may be used as an effective tool for the protection of crops against FHB pathogens.

scholarly journals Validation of a SNP-KASP marker for the Fusarium head blight resistance quantitative trait loci on chromosome 5AS

2020 ◽  
pp. 1-5
Author(s):  
Sudhakar Pandurangan ◽  
Kirby T. Nilsen ◽  
Santosh Kumar
Keyword(s):  
Quantitative Trait Loci ◽  
Fusarium Head Blight ◽  
Quantitative Trait ◽  
Polymorphic Locus ◽  
Fusarium Head Blight Resistance ◽  
Type I ◽  
Head Blight ◽  
Trait Loci ◽  
Type I Resistance ◽  
Kasp Marker

Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb5 is an important quantitative trait loci (QTL) conferring type I resistance to FHB. In this study, we accessed the usability of a Kompetitive allele-specific polymerase chain reaction (KASP) marker for the QTL on Fhb-5AS. The KASP clustering results were compared with the linked simple sequence repeat marker, wmc705 for Qfhb-5AS. Our results indicate that the single-nucleotide polymorphic locus wsnp_Ra_c24707_34262900 (IWA7777) provides reliable information for Qfhb-5AS-based resistance and would be amenable to masker-assisted selection, introgression of the resistance loci, and pyramiding of FHB resistance in wheat cultivars.

scholarly journals Effectiveness of multigenerational transfer of Sumai 3 Fusarium head blight resistance in hard red spring wheat breeding populations

2020 ◽  
Vol 100 (2) ◽  
pp. 156-174
Author(s):  
S. Berraies ◽  
R.E. Knox ◽  
R.M. DePauw ◽  
F.R. Clarke ◽  
A.R. Martin ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Type I ◽  
Head Blight ◽  
Hard Red Spring Wheat ◽  
Breeding Populations ◽  
Fhb Resistance ◽  
Selection For ◽  
Sumai 3

Several quantitative trait loci (QTL) have been identified for Fusarium head blight (FHB) resistance in the cultivar Sumai 3. Wheat breeders need to know which Sumai 3 loci are present in derived lines used as parents for effective marker-assisted selection for genetic improvement. This study was conducted to identify the loci in Sumai 3 derived parents that contribute FHB resistance in breeding populations. Three doubled haploid (DH) populations utilizing Sumai 3 derived parents, ND3085, ND744, and Alsen, were evaluated during 2007 and 2008 in FHB nurseries near Carman, MB, Ottawa, ON and Charlottetown, PE. The percentage of incidence, severity, Fusarium-damaged kernels (FDK), and deoxynivalenol (DON) accumulation were measured, and FHB index calculated. DNA markers at six FHB resistance loci detected in Sumai 3 were evaluated on the populations. For each trait, a t test was applied to means of observations pooled by parental type of each marker to determine which loci contributed to resistance. The alleles at 3BS and 5AS most frequently contributed to Type I and Type II FHB resistance, as well as to reduced FDK and DON in all three populations. Markers revealed resistance on 3BS and 5AS in Alsen, ND3085, and ND744, on 3BSc, 4D, and 6BS in ND744, on 4D in ND3085, and on 6BS in Alsen. In some environments, the susceptible parent Infinity contributed minor QTL on 2D, 3BSc, and 6BS. Likewise, Helios contributed minor QTL on 5AS and 6BS.

scholarly journals Characterisation of fusarium head blight resistance located on chromosome 4A of Triticum macha

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 580-582 ◽  
Author(s):  
A. Steed ◽  
E. Chandler ◽  
M. Thomsett ◽  
J. Carter ◽  
S. Faure ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Marker Assisted Selection ◽  
Single Gene ◽  
Fusarium Head Blight Resistance ◽  
Type I ◽  
Head Blight ◽  
Yield Trait ◽  
Fhb Resistance ◽  
Wheat Lines ◽  
Triticum Macha

Chromosome 4A of Triticum macha carries resistance to Fusarium head blight (FHB). Double haploid lines (DH) of T. macha 4A were used to determine the type of resistance and location of the gene(s). FHB resistance and yield trait data collected over two seasons following spray and point inoculation, indicate that the resistance is of type I and is probably conferred by a single gene. The resistance was mapped with microsatellite markers to a small area of the T. macha 4A chromosome flanked by markers gwm 610 and gwm 165. This could greatly facilitate future marker assisted selection work aimed at increasing resistance to FHB in other winter wheat lines.

scholarly journals Updating the Breeding Philosophy of Wheat to Fusarium Head Blight (FHB): Resistance Components, QTL Identification, and Phenotyping—A Review

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1702
Author(s):  
Akos Mesterhazy
Keyword(s):  
Fusarium Head Blight ◽  
Plant Resistance ◽  
Fusarium Head Blight Resistance ◽  
Type I ◽  
Type Ii ◽  
Head Blight ◽  
Resistance Level ◽  
Wheat Varieties ◽  
Kernel Infection ◽  
Trait Locus

Fusarium head blight has posed continuous risks to wheat production worldwide due to its effects on yield, and the fungus provides additional risks with production of toxins. Plant resistance is thought to be the most powerful method. The host plant resistance is complex, Types I–V were reported. From the time of spraying inoculation (Type I), all resistance types can be identified and used to determine the total resistance. Type II resistance (at point inoculation) describes the spread of head blight from the ovary to the other parts of the head. Therefore, it cannot solve the resistance problem alone. Type II QTL (quantitative trait locus) Fhb1 on 3BS from Sumai 3 descendant CM82036 secures about the same resistance level as Type I QTL does on 5AS and 5ASc in terms of visual symptoms, FDK (Fusarium damaged kernel), and deoxynivalenol response. Recently, increasing evidence supports the association of deoxynivalenol (DON) content and low kernel infection with FHB (Fusarium head blight) resistance (Types III and IV), as QTL for individual resistance types has been identified. In plant breeding practice, the role of visual selection remains vital, but the higher correlations for FDK/DON make it possible to select low-DON genotypes via FDK value. For phenotyping, the use of more independent inocula (isolates or mixtures) makes resistance evaluation more reliable. The large heterogeneity of the mapping populations is a serious source of underestimating genetic effects. Therefore, the increasing of homogeneity is a necessity. As no wheat varieties exist with full resistance to FHB, crops must be supported by proper agronomy and fungicide use.

scholarly journals Evaluation of type I fusarium head blight resistance of wheat using non-deoxynivalenol-producing fungi

2010 ◽  
Vol 59 (1) ◽  
pp. 147-157 ◽  
Author(s):  
N. Gosman ◽  
Srinivasachary ◽  
A. Steed ◽  
E. Chandler ◽  
M. Thomsett ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Type I ◽  
Head Blight

Comparing Genetic Variation within Red Winter Wheat Populations with and without Image-Based Optical Sorter Selection

2019 ◽  
Vol 9 (2) ◽  
pp. 266-277
Author(s):  
Hussein M. Khaeim ◽  
Anthony Clark ◽  
Tom Pearson ◽  
Dr. David Van Sanford
Keyword(s):  
Genetic Variation ◽  
Fusarium Head Blight ◽  
Heading Date ◽  
Fusarium Head Blight Resistance ◽  
Gibberella Zeae ◽  
Mass Selection ◽  
Head Blight ◽  
Red Winter Wheat ◽  
Two Populations ◽  
Head Scab

Head scab is historically a devastating disease affecting not just all classes of wheat but also barley and other small grains around the world. Fusarium head blight (FHB), or head scab, is caused most often by Fusarium graminearum (Schwabe), (sexual stage – Gibberella zeae) although several Fusarium spp. can cause the disease. This study was conducted to determine the effect of mass selection for FHB resistance using an image-based optical sorter. lines were derived from the C0 and C2 of two populations to compare genetic variation within populations with and without sorter selection. Our overall hypothesis is that sorting grain results in improved Fusarium head blight resistance. Both of the used wheat derived line populations have genetic variation, and population 1 has more than population 17. They are significantly different from each other for fusarium damged kernel (FDK), deoxynivalenol (DON), and other FHB traits. Although both populations are suitable to be grown for bulks, population 1 seems better since it has more genetic variation as well as lower FDK and DON, and earlier heading date. Lines within each population were significantly different and some lines in each population had significantly lower FDK and DON after selection using an optical sorter. Some lines had significant reduction in both FDK and DON, and some others had either FDK or DON reduction. Lines of population 1 that had significant reduction, were more numerous than in population 17, and FDK and DON reduction were greater.

Registration of CJ 8809 Wheat Germplasm with Fusarium Head Blight Resistance

2007 ◽  
Vol 1 (1) ◽  
pp. 72-74
Author(s):  
Guo-Liang Jiang ◽  
ZhaoSu Wu ◽  
ZhaoXia Chen ◽  
JiMing Wu ◽  
QiMei Xia ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Head Blight ◽  
Wheat Germplasm
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