scholarly journals First Report of Tomato leaf curl New Delhi virus Infecting Tomato in Bangladesh

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1011-1011 ◽  
Author(s):  
M. N. Maruthi ◽  
A. R. Rekha ◽  
A. Cork ◽  
J. Colvin ◽  
S. N. Alam ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
Disease Symptoms ◽  
Viral Genomes ◽  
Virus Diversity ◽  
Important Cash Crop ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Viral Sequences ◽  
Leaf Curl

Tomato is an important cash crop for resource-poor farmers and accounts for 20% of the 2 million t of vegetables grown annually in Bangladesh. Tomato cultivation is affected by Tomato leaf curl virus (ToLCV), which can cause as much as 100% yield loss. Plants exhibiting typical ToLCV disease symptoms of yellowing, severe leaf curling, and stunting were collected at Jessore, Bangladesh during September 2003. The putative virus was transmitted from tomato to tomato by the whitefly Bemisia tabaci. In two separate experiments, 100% transmission was achieved by using 10 viruliferous B. tabaci adults for each of the 20 test plants that was confirmed by comparing the symptoms on test and virus source plants. Total DNAs were extracted from the symptomatic leaves, and the putative viral genomes were amplified by polymerase chain reaction by using the Deng A and B primers (1). Sequences generated from these primers were used to design virus-specific primers that were used to obtain complete viral sequences. Full-length DNA-A (2,740 nt; GenBank Accession No. AJ875157) and DNA-B (2,688 nt; GenBank Accession No. AJ875158) sequences of a bipartite Tomato leaf curl New Delhi virus from Jessore (ToLCNDV-[Jes]) were obtained, which were most similar to the corresponding sequences of ToLCNDV-(Lucknow) (GenBank Accession No. Y16421) at 95.7% and Tomato leaf curl Gujarat virus-(Varanasi) (Gen-Bank Accession No. AY190291) at 90.6% nt identities, respectively. DNA-A sequences had only 73.2% nt identity with the previously reported monopartite Tomato leaf curl Bangladesh virus (GenBank Accession No. AF188481) (2), confirming the occurrence of mono- and bipartite bego-moviruses in Bangladesh. The virus diversity poses a challenge for ToLCVD management in Bangladesh. References: (1) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (2) S. K. Green et al. Plant Dis. 85:1286, 2001.

scholarly journals First Report of Association of Tomato leaf curl virus-New Delhi with Yellow Mosaic Disease of Luffa cylindrica in India

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
S. S. Sohrab ◽  
B. Mandal ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
New Delhi ◽  
Luffa Cylindrica ◽  
Yellow Mosaic Disease ◽  
Tomato Leaf Curl Virus ◽  
Sponge Gourd ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Sponge gourd (Luffa cylindrica), an important cucurbitaceous vegetable in India, is affected by a disease (2) causing yellow spots on newly emerged leaves, mosaic, mild leaf curling and distortion, small leaves, and misshapen fruits. Nearly 100% of sponge gourd plants were symptomatic in Delhi. Geminivirus-like particles were observed with electron microscopy of uranyl acetate-stained leaf-dip preparations of the diseased plants collected from experimental fields at the Indian Agricultural Research Institute in New Delhi during May and June of 2002. The virus was transmitted by the whitefly (Bemisia tabaci) to sponge and ridge gourd (L. acutangula) after an acquisition and inoculation access period of 24 h each. Whitefly-inoculated plants produced typical yellow mosaic symptoms and contained geminate particles. Nucleic acid extracted from the field-infected and experimentally infected plants hybridized with 32P-labeled probe to DNA-A of Indian cassava mosaic virus, suggesting association of a begomovirus. The viral DNA, isolated by the alkali denaturation method (1) from the experimentally infected sponge gourd plants, was cloned in pBS SK+ at the EcoRI site. A clone with an insert of 2,658 bp was sequenced (GenBank Accession Nos. AJ557219, AJ555488, and AY309957) which shared 89.6 to 95.1% identity with the DNA-A of different strains of Tomato leaf curl virus-New Delhi (ToLCV-NDe). The highest sequence identity (95.1%) was with the severe strain of ToLCV-NDe (GenBank Accession No. U15015). The data suggest that the begomovirus associated with the yellow mosaic disease of L. cylindrica in India is a putative strain of ToLCV-NDe. Reference: (1) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (2) A. Varma and B. K. Giri. Virus diseases. Pages 225–245 in: Cucurbits. N. M. Nayar and T. A More, eds. Oxford and IBH Publishing House Private Ltd., New Delhi, India, 1998.

scholarly journals Tomato leaf curl Karnataka virus from Bangalore, India, Appears to be a Recombinant Begomovirus

2002 ◽  
Vol 92 (6) ◽  
pp. 637-645 ◽  
Author(s):  
Orawan Chatchawankanphanich ◽  
Douglas P. Maxwell
Keyword(s):  
Particle Bombardment ◽  
Phylogenetic Trees ◽  
Tomato Leaf ◽  
New Delhi ◽  
Intergenic Regions ◽  
Biotype B ◽  
Distinct Member ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

The genome of Tomato leaf curl virus (ToLCV) from Bangalore, India, a whitefly-transmitted geminivirus, was cloned (pIND9) and sequenced. The circular DNA of 2,759 nucleotides (U38239) is organized similarly to that of other begomoviruses with monopartite genomes. Comparison of the nucleotide sequence of pIND9 with other tomato-associated begomoviruses from India (Tomato leaf curl Bangalore virus [ToLCBV, Z48182]) and Tomato leaf curl New Delhi virus-Severe (ToLCNdV-Svr, U15015) showed moderate DNA sequence identities (82 to 87%) between capsid protein (CP) genes but low identities (66 to 67%) for the intergenic regions and the replication-associated protein (Rep) genes (75 to 81% identity). Phylogenetic trees generated with nucleotide sequences of the Rep and CP genes of 26 begomoviruses indicated that this ToLCV is distinct from other begomoviruses and that it may be a recombinant virus derived from at least three different viral lineages. Tomatoes (Lycopersicon esculentum) inoculated with the cloned DNA monomer of ToLCV (pIND9) via particle bombardment developed leaf curling and yellowing symptoms. The virus was transmitted by Bemisia tabaci biotype B from tomatoes infected via particle bombardment to healthy tomatoes and by sap inoculation from infected tomatoes to tomato, Nicotiana benthamiana and N. tabacum. This ToLCV is a distinct member of the genus Begomovirus from India that differs from the previously characterized Tomato leaf curl Sadasivanagar virus isolate Bangalore 1 (L12739), ToLCBV (Z48182), ToLCBV isolate Bangalore 4 (AF165098), and the bipartite ToLCNdV (U15015, U15016). Thus, this ToLCV is named Tomato leaf curl Karnataka virus (ToLCKV).

scholarly journals First Report of Tomato leaf curl New Delhi virus Infecting Cucumber in Central Java, Indonesia

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1485-1485 ◽  
Author(s):  
T. Mizutani ◽  
B. S. Daryono ◽  
M. Ikegami ◽  
K. T. Natsuaki
Keyword(s):  
Tomato Leaf ◽  
High Nucleotide Sequence Identity ◽  
New Delhi ◽  
Natural Occurrence ◽  
Cucumis Sativus L ◽  
First Report ◽  
Viral Genomes ◽  
Central Java ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Cucumber (Cucumis sativus L.) is an important vegetable in Indonesia. Cucumber plants showing yellowy green mosaic symptoms on leaves were observed in Klaten, Central Java, Indonesia in August 2008. Total DNAs were extracted from symptomatic leaves, and the putative viral genomes were amplified by PCR with the Deng A and B primers (2). The PCR-amplified viral genomic DNA was sequenced. The remaining part of DNA-A was amplified with two primers sets (ToLCNDV-A1F 5′-ACCAACAGGCCGATGAACA-3′ and ToLCNDV-A1R 5′-TTCCCACTATCTTCCTGTGCA-3′; ToLCNDV-A2F 5′-TCGAGTGTGATRAAGAYTGCA-3′ and ToLCNDV-A2R 5′-ACTAACTAAGCATTGCAGCGTC-3′ [R = A and G, Y = C and T]) and sequenced. The remaining part of DNA-B was amplified with two primers sets (ToLCNDV-B1F 5′-ARGAGTTYMCRYYTGTGGA-3′ and ToLCNDV-B1R 5′-TKCWGTYGGTCATGTCGT-3′; ToLCNDV-B2F 5′-TCYGTCAATCKCATGTCGYGT-3′ and ToLCNDV-B2R 5′-CCTTACGCGTATAYTGTYTRGA-3′ [K = G and T, M = A and C, W = A and T]) and sequenced. Full-length DNA-A (2,739 nt; GenBank Accession No. AB613825) and DNA-B (2,690 nt; GenBank Accession No. AB613826) sequences of a bipartite Tomato leaf curl New Delhi virus (ToLCNDV) from Central Java were obtained and they were most similar to the corresponding sequences of both DNA-A and DNA-B of ToLCNDV-[cucumber:Thailand] (DNA-A, GenBank Accession No. AB330079; DNA-B, GenBank Accession No. AB330080) at 95.5 and 91.0% nucleotide identities, respectively. On the basis of high nucleotide sequence identity with ToLCNDV-[cucumber:Thailand] and the demarcation criteria in species identification (3), the virus isolate from the diseased cucumber in Central Java is considered as a variant of ToLCNDV and was accordingly named ToLCNDV-Indonesia[Indonesia:Java:Cucumber:2008] (ToLCNDV-ID[ID:Jav:Cuc:08]). Although the importance of begomovirus diseases on chili pepper (Solanaceae) is currently highly noticed in Indonesia (1), ToLCNDV was newly isolated from cucumber (Cucurbitaceae) in this study. Therefore, farmers in Indonesia should pay more attention to controlling begomovirus vectors, white flies, on Cucurbitaceae. To our knowledge, this is the first report of the natural occurrence of ToLCNDV in Indonesia. References: (1) P. J. D. Barro et al. Biol. Invas.10:411, 2008. (2) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (3) C. M. Fauquet et al. Arch. Virol. 153:783, 2008.

scholarly journals ​Molecular Characterization of Viruses Associated with Leaf Curl Symptoms on Capsicum (Bell Pepper) under Protected Cultivations in Dharwad, Karnataka

2021 ◽  
Author(s):  
C. Channakeshava. M.S. Patil. B. Gurupad ◽  
N.B. Moger
Keyword(s):  
Tomato Leaf ◽  
Bell Pepper ◽  
Ammonium Bromide ◽  
New Delhi ◽  
Tomato Leaf Curl Virus ◽  
Study Results ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

Background: Leaf curl or yellowing symptoms, typical of those caused by begomovirus infection, are commonly observed in capsicum (bell pepper) plants in polyhouses. Three capsicum samples with leaf curl and yellowing symptoms were collected from polyhouse at Hi-tech Horticulture, University of Agricultural Sciences, Dharwad, Karnataka, India, during 2017-2018. Methods: Total nucleic acid was isolated from symptomatic and non-symptomatic samples by following CTAB (Cetyl trimethyl ammonium bromide) method and PCR amplified using degenerate and coat protein gene primers of chilli and tomato leaf curl virus. The virus was confirmed by gel electrophoresis with 0.8 per cent agarose. Amplified PCR product was eluted and sent to sequencing (Chromous biotech Pvt. Ltd., Bengaluru). Result: The primer pair could amplify only Chilli leaf curl virus at ~500 bp but not Tomato leaf curl virus. Sequence homology of ChiLCV isolate form Dharwad matched with ChiLCV-Papaya-New Delhi (HM14036), ChiLCV-Chilli-Jodhpu (HM007104) and ChiLCV-Chilli-Noida (HM007114) respectively. The present study results showed that Chilli leaf curl virus is associated with the leaf curl and yellowing symptoms on capsicum under protected cultivation in Dharwad, Karnataka, India.

scholarly journals Identification of Replication Specificity Determinants in Two Strains of Tomato Leaf Curl Virus from New Delhi

1999 ◽  
Vol 73 (7) ◽  
pp. 5481-5489 ◽  
Author(s):  
Anju Chatterji ◽  
Malla Padidam ◽  
Roger N. Beachy ◽  
Claude M. Fauquet
Keyword(s):  
Binding Site ◽  
Tomato Leaf ◽  
Single Amino Acid ◽  
New Delhi ◽  
Rep Protein ◽  
Tomato Leaf Curl Virus ◽  
Severe Strain ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

ABSTRACT We used two strains of tomato leaf curl virus from New Delhi to investigate specificity in replication of their cognate genomes. The strains share 94% sequence identity and are referred to as severe and mild on the basis of symptoms on tomato and tobacco. Replication assays in tobacco protoplasts and plants showed that a single amino acid change, Asn10 to Asp in the N terminus of Rep protein, determines specificity for replication of the two strains based upon its interaction with the origin of replication (ori) sequences. The change of Asp10 to Asn in Rep protein of the mild strain coupled with point mutations at the 3rd and 10th nucleotides of the 13-mer binding site altered its replication ability, resulting in increased levels of virus accumulation. Similarly, changing Asn10 to Asp in Rep protein of the severe strain impaired replication of the virus and altered its severe phenotype in plants. Site-directed mutations made inori and Asn10 of Rep protein suggested that Asn10 recognizes the third base pair of the putative binding site sequence GGTGTCGGAGTC in the severe strain.

scholarly journals Identifikasi Begomovirus yang Berasosiasi dengan Penyakit Kuning pada Mentimun di Jawa Barat dan Bali

2016 ◽  
Vol 7 (1) ◽  
pp. 9 ◽  
Author(s):  
Rizki Haerunisa ◽  
Gede Suastika ◽  
Tri Asmira Damayanti
Keyword(s):  
Nicotiana Benthamiana ◽  
Protein Gene ◽  
Tomato Leaf ◽  
New Delhi ◽  
West Java ◽  
Ageratum Yellow Vein Virus ◽  
Squash Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

<p>ABSTRACT<br />A survey conducted from several cucumber cultivation area of West Java and Bali found some plants showing yellow mosaic, vein banding, and stunting symptoms, caused by Begomovirus infection. This study aimed to detect and determine incidence of Begomovirus on cucumber plants, and analyze variation of Begomovirus coat protein gene in West Java dan Bali. Leaf samples from 50 plants were taken randomly from each location in Sumedang, Karawang, Sukabumi (West Java) and Tabanan, Gianyar, Klungkung (Bali). Disease incidence was determined based on serological assay using specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) dan Squash leaf curl virus (SLCV). Incidence of ToLCNDV and SLCV were 28-100% and 30-80%, respectively. PCR using Begomovirus degenerate primers successfully amplified coat protein gene about ± 550bp. There were three Begomovirus species associated with yellowing disease on cucumber plant i.e Squash leaf curl China virus (SLCCNV), ToLCNDV, and Ageratum yellow vein virus (AYVV). Based on nucleotide sequences analysis, it was found that isolate SLCCNV had highest nucleotide homology with SLCCNV isolate Malaysia (EF197940) about 94.5%, and was considered as a strain “China”, whereas ToLCNDV has highest nucleotide similarity with ToLCNDV isolate Indonesia (AB613825) about 99.4% and was considered as a strain “Indonesia”. The AYVV sequences showed highest nucleotide AYVV isolate Nicotiana benthamiana from Indonesia (AB100305) about 92.1%.</p><p>Keywords: Cucumis sativus, Dot immunobinding assay, Squash leaf curl China virus, Tomato leaf curl New Delhi virus</p><p>ABSTRAK<br />Survei yang dilakukan di beberapa pertanaman mentimun di Jawa Barat dan Bali menemukan gejala mosaik kuning, daun keriting, penebalan tulang daun, dan kerdil akibat infeksi Begomovirus. Penelitian ini bertujuan untuk mendeteksi dan menghitung insidensi penyakit yang disebabkan oleh Begomovirus pada tanaman mentimun, serta menganalisis keragaman gen protein selubung Begomovirus di Jawa Barat dan Bali. Sampel daun diambil secara acak sebanyak 50 tanaman dari tiap lokasi pertanaman mentimun di Sumedang, Karawang, dan Sukabumi (Jawa Barat), Tabanan, Gianyar, dan Klungkung (Bali). Deteksi Begomovirus dan insidensi penyakit ditentukan dengan uji serologi DIBA menggunakan antiserum Tomato leaf curl New Delhi virus (ToLCNDV) dan Squash leaf curl virus (SLCV). Insidensi ToLCNDV dan SLCV berturut-turut berkisar 28-80% dan 30-80%. Deteksi dengan teknik PCR menggunakan primer universal Begomovirus berhasil mengamplifikasi gen protein selubung berukuran ± 550 pb. Hasil perunutan nukleotida menunjukkan terdapat tiga spesies Begomovirus yang menginfeksi tanaman mentimun di Jawa Barat dan Bali, yaitu Squash leaf curl China virus (SLCCNV), ToLCNDV, dan Ageratum yellow vein virus (AYVV). Isolat SLCCNV Bali memiliki kesamaan nukleotida dan asam amino terhadap isolat SLCCNV dari negara lainnya berkisar antara 89.8-94.5% dan 94.2-96.3%, dan dikelompokkan ke dalam strain “Cina”. ToLCNDV isolat Jawa Barat dan Bali memiliki kesamaan nukleotida dan asam amino berkisar antara 92.8-99.4% dan 97.3-99.4% dengan isolat ToLCNDV dari negara lainnya, tergolong ke dalam strain “Indonesia”. Gen protein selubung AYVV Bali memiliki kesamaan nukleotida dan asam amino berkisar antara 89.5-92.1% dan 94.7-95.2%, dengan kesamaan tertinggi dengan isolat AYVV asal Indonesia yang menginfeksi Nicotiana benthamiana.</p><p>Kata kunci: Cucumis sativus, Dot immunobinding assay, Squash leaf curl China virus, Tomato leaf curl New Delhi virus.</p>

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