scholarly journals Molecular, Physiological, and Host-Range Characterization of Acidovorax avenae subsp. citrulli Isolates from Watermelon and Melon in Israel

Plant Disease ◽  
2005 ◽  
Vol 89 (12) ◽  
pp. 1339-1347 ◽  
Author(s):  
Saul Burdman ◽  
Nadia Kots ◽  
Giora Kritzman ◽  
June Kopelowitz
Keyword(s):  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Pulse Field ◽  
Bacterial Isolates ◽  
Pulse Field Gel Electrophoresis ◽  
Acid Methyl ◽  
Group I ◽  
Serious Disease ◽  
Range Characterization

Bacterial fruit blotch (BFB), caused by Acidovorax avenae subsp. citrulli, is a serious disease of cucurbit plants. The first important occurrence of BFB in Israel was during 2000 to 2003 on watermelon and melon. Twelve bacterial isolates associated with these outbreaks were confirmed as A. avenae subsp. citrulli by pathogenicity assays, gas chromatography of fatty-acid methyl esters, and substrate-utilization profiles. The isolates were characterized in terms of their aggressiveness in different hosts by seed, seedling, and fruit inoculations, and according to their DNA fingerprinting profiles using pulse-field gel electrophoresis (PFGE) and repetitive-PCR approaches. Results from the present work agree with previous studies supporting the existence of two differentiated groups within A. avenae subsp. citrulli, one including strains that are more associated with watermelon (group II), the other consisting of strains that are usually associated with nonwatermelon cucurbits (group I). This study indicates that isolates from both groups have been introduced to Israel. PFGE analysis revealed that the 12 analyzed isolates can be divided into five different haplotypes, of which four were previously unreported. Additional differentiating features between group I and II strains are presented.

scholarly journals Investigating Intraspecific Variation of Acidovorax avenae subsp. citrulli Using DNA Fingerprinting and Whole Cell Fatty Acid Analysis

2000 ◽  
Vol 90 (2) ◽  
pp. 191-196 ◽  
Author(s):  
R. R. Walcott ◽  
D. B. Langston ◽  
F. H. Sanders ◽  
R. D. Gitaitis
Keyword(s):  
United States ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
The United States ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Pulse Field ◽  
Pulse Field Gel Electrophoresis ◽  
Group I

To assess the diversity of Acidovorax avenae subsp. citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of SpeI-digested DNA and gas chromatographic analysis of fatty acid methyl esters. Twenty-nine unique DNA fragments resulted from DNA digestion, and 14 distinct haplotypes were observed. Based on cluster analysis, two subgroups, I and II, were recognized, which accounted for 84.8% (eight haplotypes) and 15.2% (six haplotypes) of the strains, respectively. Results of cellular fatty acid analysis varied quantitatively and qualitatively for the A. avenae subsp. citrulli strains and supported the existence of the two subgroups. Group I includes strains from cantaloupe and pumpkin as well as the ATCC type strain, which was first described in the United States in 1978, whereas group II represents the typical watermelon fruit blotch-causing strains that appeared in the mainland United States in 1989. Knowledge of the two A. avenae subsp. citrulli groups may be useful in screening for watermelon fruit blotch resistance.

QSPR Modeling is Able to Predict Retention Times of Fatty Acids Using Simple Molecular Descriptors

2017 ◽  
Vol 2 (1) ◽  
pp. 35-43
Author(s):  
Mariene H. Duarte ◽  
Matheus P. Freitas ◽  
Cleiton Nunes
Keyword(s):  
Fatty Acid Methyl Esters ◽  
Molecular Descriptors ◽  
Methyl Esters ◽  
Relevant Information ◽  
Chromatographic Retention ◽  
Double Bonds ◽  
Acid Methyl ◽  
Qspr Modeling ◽  
Mlr Model

A QSPR modeling was carried out to predict the chromatographic retention times of a series of fatty acid methyl esters (FAME) widely used as standard in the characterization of lipids from agricultural and food products. Number of carbons, total double bonds, position of double bonds and geometric isomerism were used as descriptors to generate a Multiple Linear Regression (MLR) model. The best model yielded an RMSE = 0.167 and R2 = 0.999 for the calibration set, and RMSE = 0.151 and R2 = 1.000 for the test set. Number of carbons and total double bonds were the most important descriptors, according to the regression coefficients, but position of double bonds and isomerism cannot be neglected as they provide relevant information to improve the accuracy of the predicted property.

Chromatographic characterization of triacylglycerides and fatty acid methyl esters in microalgae oils for biodiesel production

2013 ◽  
Vol 5 (5) ◽  
pp. 053111 ◽  
Author(s):  
A. T. Soares ◽  
B. F. Silva ◽  
L. L. Fialho ◽  
M. A. G. Pequeno ◽  
A. A. H. Vieira ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Biodiesel Production ◽  
Acid Methyl ◽  
Chromatographic Characterization

Formation and Characterization of Fully Dilutable Microemulsion with Fatty Acid Methyl Esters as Oil Phase

2015 ◽  
Vol 3 (3) ◽  
pp. 443-450 ◽  
Author(s):  
Wanxu Wang ◽  
Hongtu Wei ◽  
Zhiping Du ◽  
Xiumei Tai ◽  
Guoyong Wang
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Acid Methyl

Rheological and Some Fuel Properties of Fatty Acid Methyl Esters of the Oils of Palm, Palm Kernel and Groundnut

2011 ◽  
Vol 367 ◽  
pp. 343-352
Author(s):  
V.C. Eze ◽  
Paul M. Ejikeme ◽  
O.D. Onukwuli
Keyword(s):  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Palm Kernel ◽  
Quality Parameters ◽  
Fuel Quality ◽  
Free Energies ◽  
Groundnut Oil ◽  
Kernel Oil ◽  
Acid Methyl

Production and characterization of fatty acids methyl esters (FAME) from palm oil (PO), palm kernel oil (PKO) and groundnut oil (GNO) were carried out in this study. Optimal amount of NaOH catalyst required for the transesterification of the degummed and refined oils were determined. Some rheological and fuel quality parameters of the FAME obtained from batch transesterification of each oil, petrodiesel and their blends, were determined. The optimal catalyst weights obtained, based on oil, were 0.40, 0.48 and 0.56 (%wt/v) for GNO, PKO and PO, respectively. The FAME produced had viscosities of 4.45, 3.44, and 4.11mm2/s as compared to the 38.84, 37.51 and 36.92mm2/s obtained for the parent oils PO, PKO, and GNO, respectively. The measured viscosity of petrodiesel was 2.85mm2/s. Thermodynamic free energies of activation for flow (∆Gvis) for the FAME samples were 18.1, 13.4 and 14.7KJ/mol for PO, PKO, GNO, respectively, and 10.1KJ/mol for petrodiesel.

scholarly journals GC-MS Characterization of Unsweetened and Sweetened “Kunun-zaki” Beverage and Pulp

2020 ◽  
pp. 25-39
Author(s):  
Esienanwan Esien Efiong ◽  
Jonathan Maduka Nwaedozie ◽  
Abraham Ehinomhen Ubhenin ◽  
Emmanuel Hala Kwon- Ndung
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Octadecadienoic Acid ◽  
Methyl Esters ◽  
Hexadecanoic Acid ◽  
Sweet Potatoes ◽  
Acid Methyl ◽  
Soxhlet Apparatus ◽  
Significant Health

Aims: To characterize unsweetened and sweetened “kunun-zaki” drink/pulp using GC-MS. Study Design: The experimental groups were divided into four: unsweetened “kunun-zaki” drink, sweetened “kunun-zaki” drink, unsweetened “kunun-zaki” pulp and sweetened “kunun-zaki” pulp respectively. Place of Study: Department of Biochemistry, Federal University of Lafia, Nasarawa State and Central Diagnostic and Research Laboratory, Ilorin, Nigeria. Methodology: The processes involved sorting, cleaning, soaking, decanting, washing, milling, gelatinization, fermentation, and sieving. Unsweetened “kunun-zaki” beverage was made from millet, sorghum, soybean, dried sweet potatoes, germinated rice, ginger, and cloves. The same process was followed for the sweetened variety, with addition of dried dates. After sieving to collect “kunun-zaki” beverage, the remnant was the pulp. Hydrodistillation and Soxhlet apparatus were used to extract the essential oils, and fatty acids respectively. Fatty acid methyl esters of the samples were then analysed using GC-MS. Results: The GC chromatogram identified three constituents in unsweetened “kunun-zaki” drink. The dominant constituents were 9,12-octadecadienenoic acid (z,)- (73.36%), and n-hexadecanoic acid (19.31%). Eleven compounds were identified in sweetened “kunun-zaki” drink. The most abundant was 9, 12-octadecadienoic acid (Z,Z)- 54.062%, followed by n-hexadecanoic acid (17.278%). Twenty-one peaks corresponding to eighteen compounds were detected in unsweetened “kunun-zaki” pulp. The dominant constituents were gingerol (20.262%), 1H-3a,7-methanoazulene,2,3,4,7,8,8a-hexahydro-3,6,8,8-teramethyl-,[3R-(3α,3aβ,7 β,8aα)]- (19.925%),  2-Butanone,4-(4-hydroxy-3-methoxyphenyl)- (15.59%), and cedrene (10.977%). For sweetened “kunun-zaki” pulp, four compounds were detected. The dominant compounds being 9,12-octadecadienoic acid (z,z)- (68.664%), n-hexadecanoic acid (18.654%), and oleic acid (10.199%). Conclusion: The results showed safe fatty acid profile of all samples for different health status. Other components showed bioactivities with significant health benefits. This study identified compounds which validate the medicinal claims of “kunun-zaki.” This is the first documentation of GC-MS profiling of “kunun-zaki” parts.

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