scholarly journals Investigating Intraspecific Variation of Acidovorax avenae subsp. citrulli Using DNA Fingerprinting and Whole Cell Fatty Acid Analysis

2000 ◽  
Vol 90 (2) ◽  
pp. 191-196 ◽  
Author(s):  
R. R. Walcott ◽  
D. B. Langston ◽  
F. H. Sanders ◽  
R. D. Gitaitis
Keyword(s):  
United States ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
The United States ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Pulse Field ◽  
Pulse Field Gel Electrophoresis ◽  
Group I

To assess the diversity of Acidovorax avenae subsp. citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of SpeI-digested DNA and gas chromatographic analysis of fatty acid methyl esters. Twenty-nine unique DNA fragments resulted from DNA digestion, and 14 distinct haplotypes were observed. Based on cluster analysis, two subgroups, I and II, were recognized, which accounted for 84.8% (eight haplotypes) and 15.2% (six haplotypes) of the strains, respectively. Results of cellular fatty acid analysis varied quantitatively and qualitatively for the A. avenae subsp. citrulli strains and supported the existence of the two subgroups. Group I includes strains from cantaloupe and pumpkin as well as the ATCC type strain, which was first described in the United States in 1978, whereas group II represents the typical watermelon fruit blotch-causing strains that appeared in the mainland United States in 1989. Knowledge of the two A. avenae subsp. citrulli groups may be useful in screening for watermelon fruit blotch resistance.

scholarly journals Molecular, Physiological, and Host-Range Characterization of Acidovorax avenae subsp. citrulli Isolates from Watermelon and Melon in Israel

Plant Disease ◽  
2005 ◽  
Vol 89 (12) ◽  
pp. 1339-1347 ◽  
Author(s):  
Saul Burdman ◽  
Nadia Kots ◽  
Giora Kritzman ◽  
June Kopelowitz
Keyword(s):  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Pulse Field ◽  
Bacterial Isolates ◽  
Pulse Field Gel Electrophoresis ◽  
Acid Methyl ◽  
Group I ◽  
Serious Disease ◽  
Range Characterization

Bacterial fruit blotch (BFB), caused by Acidovorax avenae subsp. citrulli, is a serious disease of cucurbit plants. The first important occurrence of BFB in Israel was during 2000 to 2003 on watermelon and melon. Twelve bacterial isolates associated with these outbreaks were confirmed as A. avenae subsp. citrulli by pathogenicity assays, gas chromatography of fatty-acid methyl esters, and substrate-utilization profiles. The isolates were characterized in terms of their aggressiveness in different hosts by seed, seedling, and fruit inoculations, and according to their DNA fingerprinting profiles using pulse-field gel electrophoresis (PFGE) and repetitive-PCR approaches. Results from the present work agree with previous studies supporting the existence of two differentiated groups within A. avenae subsp. citrulli, one including strains that are more associated with watermelon (group II), the other consisting of strains that are usually associated with nonwatermelon cucurbits (group I). This study indicates that isolates from both groups have been introduced to Israel. PFGE analysis revealed that the 12 analyzed isolates can be divided into five different haplotypes, of which four were previously unreported. Additional differentiating features between group I and II strains are presented.

Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography

2016 ◽  
Vol 495 ◽  
pp. 6-8 ◽  
Author(s):  
Ken'ichi Ichihara ◽  
Chihiro Kohsaka ◽  
Naohiro Tomari ◽  
Tamami Kiyono ◽  
Jun Wada ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Fatty Acid Analysis ◽  
Acid Methyl

Ionic liquids as stationary phases for fatty acid analysis by gas chromatography

The Analyst ◽  
2017 ◽  
Vol 142 (24) ◽  
pp. 4601-4612 ◽  
Author(s):  
C. Fanali ◽  
G. Micalizzi ◽  
P. Dugo ◽  
L. Mondello
Keyword(s):  
Gas Chromatography ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Stationary Phases ◽  
Methyl Esters ◽  
Fatty Acid Analysis ◽  
Gc Analysis ◽  
Acid Methyl

The present paper provides an overview of the application of ionic liquid (IL) columns for GC analysis of fatty acid methyl esters (FAMEs).

scholarly journals FATTY ACIDS ANALYSIS AND CHEMOTAXONOMIC CONSIDERATIONS OF MALVOIDEAE (MALVACEAE) SPECIES

Química Nova ◽  
2020 ◽  
Author(s):  
Diégina Fernandes ◽  
Otemberg Chaves ◽  
Yanna Teles ◽  
Maria Agra ◽  
Maria Vieira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Euclidean Distance ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Principal Component ◽  
Fatty Acid Analysis ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Fatty Acids Analysis

Previous researches showed that fatty acids analysis might be a useful tool to support the taxonomic investigation. In this approach the fatty acids content of ten Malvoideae species was analyzed and its chemotaxonomic significance has been investigated. The aerial parts of the species were collected in the Northeast of Brazil and their fatty acid methyl esters were analyzed by gas chromatography with flame ionization detector. The chemometric analysis consisted of principal component analysis (PCA) and hierarchical clustering analysis (HCA) with the euclidean distance between the samples given by the Ward.D2 algorithm. This is the first report of fatty acids from Wissadula peripocifolia, Herissantia crispa, Bakeridesia pickelii, Sidastrum micranthum, Pavonia cancellata and Pavonia malacophylla. The results showed the predominance of palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids in the studied species. By the PCA and HCA analysis, the fatty acid composition was able to distinguish the species Herissantia crispa and Pavonia malacophylla. Our findings showed a chemotaxonomic proximity among species from different genera reflecting the taxonomic and phylogenetic closeness previously demonstrated by molecular investigations on Malvoidae species. Furthermore, our results demonstrated that the fatty acid analysis may be an interesting tool to support the taxonomic investigations on Malvoideae species.

Identification of foodborne bacteria by infrared spectroscopy using cellular fatty acid methyl esters

2003 ◽  
Vol 55 (3) ◽  
pp. 709-716 ◽  
Author(s):  
P Whittaker ◽  
M.M Mossoba ◽  
S Al-Khaldi ◽  
F.S Fry ◽  
V.C Dunkel ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Cellular Fatty Acid ◽  
Foodborne Bacteria ◽  
Acid Methyl

A Consumer’s Guide to Eggs

EDIS ◽  
2013 ◽  
Vol 2013 (11) ◽  
Author(s):  
Jeanine Beatty ◽  
Karla Shelnutt ◽  
Gail P. A. Kauwell
Keyword(s):  
United States ◽  
Fatty Acid ◽  
New World ◽  
The United States ◽  
Omega 3 ◽  
Bottom Line ◽  
Christopher Columbus ◽  
Food Stores ◽  
Omega 3 Fatty Acid ◽  
Chicken Eggs

People have been eating eggs for centuries. Records as far back as 1400 BC show that the Chinese and Egyptians raised birds for their eggs. The first domesticated birds to reach the Americas arrived in 1493 on Christopher Columbus' second voyage to the New World. Most food stores in the United States offer many varieties of chicken eggs to choose from — white, brown, organic, cage free, vegetarian, omega-3 fatty acid enriched, and more. The bottom line is that buying eggs is not as simple as it used to be because more choices exist today. This 4-page fact sheet will help you understand the choices you have as a consumer, so you can determine which variety of egg suits you and your family best. Written by Jeanine Beatty, Karla Shelnutt, and Gail Kauwell, and published by the UF Department of Family Youth and Community Sciences, November 2013. http://edis.ifas.ufl.edu/fy1357

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