scholarly journals First Confirmed Report that Puccinia horiana, Causal Agent of Chrysanthemum White Rust, Can Overwinter in Pennsylvania

Plant Disease ◽  
2012 ◽  
Vol 96 (9) ◽  
pp. 1381-1381 ◽  
Author(s):  
G. O'Keefe ◽  
D. D. Davis
Keyword(s):  
United States ◽  
Molecular Analysis ◽  
Tap Water ◽  
Signs And Symptoms ◽  
The United States ◽  
Causal Agent ◽  
Controlled Environment ◽  
White Rust ◽  
Environment Chamber ◽  
Puccinia Horiana

Chrysanthemum white rust (CWR) is a quarantine-significant pest in the United States (Title 7, Code of Federal Regulations, Part 319.37-2). The causal agent of CWR, Puccinia horiana Henn., is an autoecious, microcyclic rust that is pathogenic on chrysanthemum species (Chrysanthemum spp.) and close relatives within the family Asteraceae. CWR is indigenous to Japan, where it was first reported in 1895 (4). By the 1960s, CWR was found throughout Europe and later spread to Africa, Oceana, South America, and other parts of Asia. In North America, CWR was reported in Mexico and in the United States (New Jersey and Pennsylvania [1977], Oregon and Washington [1990], and California [1991]). Additional detections of CWR were later reported in 22 Pennsylvania counties (2004, 2006 to 2010) (3). These later Pennsylvania reports stated that eradication was attempted at some sites, but unconfirmed observations suggested that the rust pathogen might overwinter in volunteer plants (3). Since “CWR is known to overwinter in Europe where chrysanthemums overwinter (average minimum temperatures ranging from –10°F to 10°F)” (2), the unconfirmed Pennsylvania observations prompted us to determine if P. horiana can overwinter in Pennsylvania. During October 2010, we identified CWR on perennial mums planted at six outdoor garden locations in University Park, PA. Symptomatic plants were quarantined and eradication attempted. Eradicated sites were routinely surveyed and CWR confirmed in July 2011 on volunteer plants at two of the originally infested sites. An additional outdoor garden site with CWR was observed in State College, PA, during October 2011 and eradication attempted. The three infested sites were surveyed throughout the fall and winter of 2011 to 2012. During February 2012, two asymptomatic volunteer plants arising from root pieces were collected from each of the three sites. Each sample was washed with tap water to remove excess soil, examined morphologically, surface sterilized with 10% bleach, and divided into two subsamples. One subsample from each site was divided into crown and root portions and DNA extracted using a Qiagen DNeasy Plant Mini Kit. Molecular analysis was performed using modifications of published primers ITS 5 and Rust1 (1,4). Puccinia horiana was detected in plant roots from one site and in plant crowns from two sites. The remaining two subsamples from each site were transplanted into sterilized potting soil and placed in a clean controlled environment chamber at 18°C and 85% relative humidity (RH). After 6 weeks, six actively growing plants were transferred to a second clean controlled environment chamber at 17°C and 90 to 100% RH. On 6 April 2012, CWR symptoms and signs were confirmed morphologically on two plants that had been removed from one site. On 19 April 2012, CWR signs and symptoms were confirmed morphologically and by molecular analysis on leaves of volunteer plants at one University Park site. DNA extractions were sequenced and shared a 100% maximum identity to a known P. horiana accession (EU816920.1) in GenBank. To our knowledge, this is the first confirmed report of P. horiana overwintering in Pennsylvania. References: (1) H. Alaei et al. Mycol. Res. 113:668, 2009. (2) Anon. Chrysanthemum White Rust Bulletin, Syngenta Flowers Inc., Gilroy, CA, 2010. (3) S. Kim et al. Phytopathology 101:S91, 2011. (4) K. Pedley. Plant Dis. 93:1252, 2009.

scholarly journals Evidence for Systemic Infection by Puccinia horiana, Causal Agent of Chrysanthemum White Rust, in Chrysanthemum

2015 ◽  
Vol 105 (1) ◽  
pp. 91-98 ◽  
Author(s):  
M. R. Bonde ◽  
C. A. Murphy ◽  
G. R. Bauchan ◽  
D. G. Luster ◽  
C. L. Palmer ◽  
...  
Keyword(s):  
United States ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Mesophyll Cell ◽  
The United States ◽  
Causal Agent ◽  
Control Measures ◽  
Mesophyll Cells ◽  
White Rust ◽  
Puccinia Horiana

Puccinia horiana, causal agent of the disease commonly known as chrysanthemum white rust (CWR), is a quarantine-significant fungal pathogen of chrysanthemum in the United States and indigenous to Asia. The pathogen was believed to have been eradicated in the United States but recently reappeared on several occasions in northeastern United States. The objective of the study presented here was to determine whether P. horiana could systemically infect chrysanthemum plants, thus providing a means of survival through winters. Scanning and transmission electron microscopy revealed the development of P. horiana on the surface and within leaves, stems, or crowns of inoculated chrysanthemum plants artificially exposed to northeastern U.S. winter temperatures. P. horiana penetrated leaves directly through the cuticle and then colonized the mesophyll tissue both inter- and intracellularly. An electron-dense material formed at the interface between fungal and host mesophyll cells, suggesting that the pathogen adhered to the plant cells. P. horiana appeared to penetrate mesophyll cell walls by enzymatic digestion, as indicated by the absence of deformation lines in host cell walls at penetration sites. The fungus was common in vascular tissue within the infected crown, often nearly replacing the entire contents of tracheid cell walls. P. horiana frequently passed from one tracheid cell to an adjacent tracheid cell by penetration either through pit pairs or nonpitted areas of the cell walls. Individual, presumed, fungal cells in mature tracheid cells of the crown and stems arising from infected crowns suggested that the pathogen might have been moving at least partially by means of the transpiration stream. The demonstration that chrysanthemum plants can be systemically infected by P. horiana suggests that additional disease control measures are required to effectively control CWR.

Sporulation Capacity and Longevity of Puccinia horiana Teliospores in Infected Chrysanthemum Leaves

2013 ◽  
Vol 14 (1) ◽  
pp. 11 ◽  
Author(s):  
Morris R. Bonde ◽  
Cristi L. Palmer ◽  
Douglas G. Luster ◽  
Susan E. Nester ◽  
Jason M. Revell ◽  
...  
Keyword(s):  
United States ◽  
Fungal Pathogen ◽  
Local Environment ◽  
The United States ◽  
First Report ◽  
White Rust ◽  
Teliospore Germination ◽  
Sporulation Capacity ◽  
Leaf Necrosis ◽  
Puccinia Horiana

Puccinia horiana Henn., a quarantine-significant fungal pathogen and causal agent of chrysanthemum white rust (CWR), was first discovered in the United States in 1977 and later believed to have been eradicated. Recently, however, the disease has sporadically reappeared in the northeastern US. Possible explanations for the reappearance include survival of the pathogen in the local environment, and reintroduction from other locations. To determine the possibility that the pathogen might be overwintering in the field, we undertook the study described here. Results from the study showed that P. horiana teliospores, imbedded in infected leaves, were capable of sporulating 2 weeks after inoculation, and this capacity continued until the leaf became necrotic and desiccated. This is the first report of the extreme susceptibility of P. horiana teliospores to leaf necrosis and desiccation and suggests that field infections following winter are unlikely to originate from teliospores. Teliospore germination on excised leaves was shown to be inhibited by light. Accepted for publication 3 April 2013. Published 23 August 2013.

Viability of Puccinia horiana Teliospores Under Various Environmental Conditions

2014 ◽  
Vol 15 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Morris R. Bonde ◽  
Cristi L. Palmer ◽  
Douglas G. Luster ◽  
Susan E. Nester ◽  
Jason M. Revell ◽  
...  
Keyword(s):  
United States ◽  
Fungal Pathogen ◽  
The United States ◽  
Northeastern United States ◽  
Plant Debris ◽  
White Rust ◽  
Winter Conditions ◽  
Dry Soil ◽  
Puccinia Horiana ◽  
Sensitive Method

Puccinia horiana Henn. is a quarantine-significant fungal pathogen and causal agent of chrysanthemum white rust (CWR). The pathogen and disease were first discovered in the United States in 1977 and quickly eradicated. During the early 1990s, CWR reemerged in several instances, but in each instance was declared eradicated. However, since approximately 2004 CWR has reappeared at an accelerated frequency. This has suggested that either P. horiana is entering the country more frequently from foreign locations or that P. horiana is now established in the field, implying that spores are capable of surviving winter conditions in plant debris or soil. As a result of the possibility that the pathogen has become established in the United States, we initiated several lines of research. The objectives of the study reported here were: (i) develop a better and more sensitive method to measure teliospore longevity; and (ii) determine if the pathogen is able to survive northeastern winters as viable teliospores. Results from the study showed that teliospores survived in the greenhouse a maximum of 28 days in dry soil and 7 days in moist soil. In a growth chamber simulating winter temperature conditions in the northeastern United States, teliospores survived a maximum of 35 days. It was concluded that P. horiana teliospores are not able to survive through typical northeastern U.S. winters. Accepted for publication 9 January 2014. Published 18 March 2014.

scholarly journals First Report of the Presence of Albugo tragopogonis on Cineraria maritima in Italy

Plant Disease ◽  
2003 ◽  
Vol 87 (4) ◽  
pp. 450-450 ◽  
Author(s):  
A. Garibaldi ◽  
A. Minuto ◽  
D. Bertetti ◽  
M. L. Gullino
Keyword(s):  
United States ◽  
Average Diameter ◽  
The United States ◽  
Causal Agent ◽  
Adaxial Surface ◽  
First Report ◽  
White Rust ◽  
Plastic Bags ◽  
Commercial Farms ◽  
Cineraria Maritima

Cineraria maritima L. (synonym Senecio cineraria DC.), commonly known as dusty-miller, is grown in Italy for landscape use in parks and gardens. In the spring of 2001, severe outbreaks of a previously unknown disease were observed in commercial farms located in northern Italy. Leaves of infected plants showed several sori on the abaxial surface, progressing to the adaxial surface, and often in the interveinal areas. On the adaxial surface of leaves, chlorotic areas developed and eventually turned brown. Severely infected leaves wilted, but remained attached to the stem. Signs of the fungus were present as whitish and catenulate sporangia emerging from the sori. Sporangia, organized in chains, had an average diameter of 20.5 × 26.5 μm. On the basis of the microscopic observations, the causal agent of the disease was identified as Albugo tragopogonis. Pathogenicity was confirmed by inoculating leaves of healthy C. maritima plants with a sporangial suspension (5 × 102 sporangia per ml) obtained from infected plants. Noninoculated plants served as a control. Plants were kept covered with plastic bags for 72 h and maintained at 15°C. After 10 days, typical symptoms of white rust developed on inoculated plants starting from the basal leaves. Within 30 days, affected leaves were completely wilted. Microscopic examination of sporangia within sori verified the pathogen to be A. tragopogonis. No symptoms developed on the control plants. A. tragopogonis has been reported as the causal agent of white rust on several species belonging to the genus Senecio in the United States (1). In New Zealand, the presence of A. tragopogonis was reported on the genus Cineraria in 1959 (2). To our knowledge, this is the first report of the presence of white rust on Cineraria maritima in Italy. References: (1) D. F. Farr et al. Fungi on Plants and Plant Products in the United States. The American Phytopathological Society, St Paul, MN, 1989. (2) J. M. Dingley. N. Z. J. Agric. Res. 2:380, 1959.

scholarly journals Molecular Analysis ofEscherichia colifrom Retail Meats (2002–2004) from the United States National Antimicrobial Resistance Monitoring System

2009 ◽  
Vol 49 (2) ◽  
pp. 195-201 ◽  
Author(s):  
James R. Johnson ◽  
James S. McCabe ◽  
David G. White ◽  
Brian Johnston ◽  
Michael A. Kuskowski ◽  
...  
Keyword(s):  
United States ◽  
Antimicrobial Resistance ◽  
Monitoring System ◽  
Molecular Analysis ◽  
The United States ◽  
Resistance Monitoring ◽  
Retail Meats

Abdominal Pain

2018 ◽  
pp. 327-331
Author(s):  
Elizabeth DeVos
Keyword(s):  
United States ◽  
Signs And Symptoms ◽  
Standard Of Care ◽  
Clinical Decision ◽  
Operative Management ◽  
The United States ◽  
Potential Utility ◽  
Treatment Protocols ◽  
Common Presentation ◽  
Treatment Risks

This case demonstrates a common presentation of appendicitis including frequent signs and symptoms and classic examination findings. Options for diagnostic imaging are reviewed. Clinical decision scores may assist in risk stratification, which may be particularly useful in austere or low-resource settings. The discussion introduces the concept of “antibiotics first” treatment for appendicitis including a discussion of patients who are poor candidates for such treatment, risks for need of future operative management, and proposed treatment protocols. While surgical management remains the standard of care in the United States, this case discusses potential utility for an “antibiotics first” protocol when definitive surgical treatment is not readily available.

scholarly journals Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170782 ◽  
Author(s):  
David Eduardo Torres ◽  
Reyna Isabel Rojas-Martínez ◽  
Emma Zavaleta-Mejía ◽  
Patricia Guevara-Fefer ◽  
G. Judith Márquez-Guzmán ◽  
...  
Keyword(s):  
Cladosporium Cladosporioides ◽  
Causal Agent ◽  
White Rust ◽  
Puccinia Horiana

scholarly journals Baseline Characteristics of Patients with Paroxysmal Nocturnal Hemoglobinuria Identified in the Department of Defense Database

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 5830-5830
Author(s):  
Marianne Ulcickas Yood ◽  
Susan Jick ◽  
Catherine Vasilakis-Scaramozza ◽  
Bonnie M.K. Donato ◽  
Ioannis Tomazos ◽  
...  
Keyword(s):  
United States ◽  
Aplastic Anemia ◽  
Department Of Defense ◽  
Military Service ◽  
Signs And Symptoms ◽  
The United States ◽  
United States Government ◽  
Surveillance Program ◽  
States Government ◽  
Equity Ownership

Abstract Background: Rare diseases are often characterized by misdiagnosis resulting in delays in critical and potentially lifesaving treatment. Awareness of first signs and symptoms of rare diseases can provide clinical evidence for early and accurate diagnosis. Medical records are the primary source of clinical information from first signs and symptoms to key clinical disease related events, yet access to records can be difficult and time consuming. We conducted a study using a large electronic medical record (EMR) and claims database to identify and describe characteristics of Paroxysmal Nocturnal Hemoglobinuria (PNH) patients at the time of their PNH diagnosis. We present baseline findings of the PNH population identified in the Department of Defense (DOD) healthcare system, where all clinical details were readily available. The DOD healthcare system is a US-based, longitudinal EMR and claims database with health information on approximately 10 million active beneficiaries throughout the country. Methods: We identified all people in the DOD database from January 01, 2007 through May 31, 2017 who had an ICD-10-CM code for PNH or a NDC or HCPCS code for eculizumab, the drug used to treat PNH. There is no ICD-9-CM code for PNH. Cases had no other indication for eculizumab use and had to have appropriate symptoms, comorbidities or lab results to be considered a case. We reviewed the electronic record for each patient and classified each as definite/likely, probable/possible or unlikely PNH based on attributes of the available health data including codes for eculizumab, flow cytometry, lab results, hemoglobinuria, aplastic anemia, pancytopenia and other comorbidities. Our ruling was validated by medical record review by a clinical expert for all equivocal cases and a sample of definite/likely cases. Individuals classified as unlikely PNH were excluded. Patient characteristics at PNH diagnosis are presented using descriptive statistics. Results: We identified 73 PNH patients (55% female) after review of all available electronic data; 41 had a diagnosis of PNH and received eculizumab, 17 had a diagnosis of PNH only, and 15 received eculizumab only. From these, 61 patients were determined to have definite/likely PNH and 12 had probable/possible PNH. The use of eculizumab ranged from 0 to 378 (median=18) prescriptions, dependent in part on the amount of follow-up in the database. There were 19 patients (26%) who had prevalent PNH and 54 (74%) who were newly diagnosed with PNH during the study period. Among newly diagnosed patients, the median age at PNH diagnosis was 46 years (range 13 - 86). Most patients had codes for hemoglobinuria at or before the PNH diagnosis date (69%), and many had codes for aplastic anemia (46%), thrombocytopenia (56%), and pancytopenia (28%). At any time after the PNH diagnosis, 85% had unspecified anemia, 52% had aplastic anemia, 53% had thrombocytopenia, and 33% had pancytopenia codes in their records. See table. Conclusion: From a population of around 10 million actively enrolled patients, across a 10-year study period, we identified 73 patients with PNH. Data from these patients will be used to identify patterns of health encounters leading up to diagnosis and relevant outcomes following diagnosis. This information can be used to help diagnose other patients with this rare disease and to improve their medical outcomes. Disclaimer Statement: Research data were derived from an approved Naval Medical Center, Portsmouth, VA IRB protocol (NMCP.2017.0080). The views expressed in this abstract are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense or the United States Government. Copyright Notice: CAPT Brian Feldman is a military service member. This work was prepared as part of his official duties. Title 17 U.S.C. 105 provides that 'Copyright protection under this title is not available for any work of the United States Government.' Title 17 U.S.C. 101 defines a United States Government work as a work prepared by a military service member or employee of the United States Government as part of that person's official duties. Table. Table. Disclosures Ulcickas Yood: Alexion Pharmaceuticals, Inc.: Other: Employee of EpiSource, LLC, which was contracted by Alexion Pharmaceuticals, Inc. EpiSource had the final decision on content. . Jick:Alexion Pharmaceuticals, Inc.: Other: Employee of the Boston Collaborative Drug Surveillance Program, which was contracted and paid by Alexion Pharmaceuticals, Inc. to work on a study of PNH using DOD data. . Vasilakis-Scaramozza:Alexion Pharmaceuticals, Inc.: Other: Employee of the Boston Collaborative Drug Surveillance Program, which was contracted and paid by Alexion Pharmaceuticals, Inc. to work on a study of PNH using DOD data.. Donato:Alexion Pharmaceuticals Inc: Employment, Equity Ownership. Tomazos:Alexion Pharmaceuticals, Inc.: Employment, Equity Ownership. L'Italien:Alexion Pharmaceuticals, Inc.: Equity Ownership, Other: Former employee and current stockholder of Alexion Pharmaceuticals, Inc. . Sicignano:Alexion Pharmaceuticals, Inc.: Other: Employee of Health ResearchTx, which has a business relationship with Alexion Pharmaceuticals, Inc.. Feldman:Alexion Pharmaceuticals, Inc.: Other: Employee, Department of Navy, United States Government..

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