scholarly journals First Report of Potato leaf roll virus (PLRV) Naturally Occurring on Jute (Corchorus olitorius) in India

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1592-1592 ◽  
Author(s):  
C. Biswas ◽  
P. Dey ◽  
S. Mitra ◽  
A. Bera ◽  
S. Satpathy ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Leaf Roll ◽  
Potato Leaf Roll Virus ◽  
Corchorus Olitorius ◽  
Specific Primer ◽  
Potato Leaf ◽  
First Report ◽  
Stunted Growth ◽  
Naturally Occurring

Jute (Corchorus olitorius L.) is an important bast fiber crop that is mainly grown in the Southeast Asian countries like India, Bangladesh, Nepal, China, Indonesia, Thailand, Myanmar, and a few South American countries. In June 2013, symptoms suggestive of a viral disease were noticed on jute (cv. JRO524) in an experimental field of the CRIJAF research farm, Barrackpore, India, and the incidence of the disease was less than 2%. The infected plants showed stunted growth and short height. Mostly the upper leaves elongated with curling and coiling of lamina. Puckering and shoe string effect were also noticed. Petioles and stipules of the affected leaves were exceptionally longer. Although initially the incidence was low, it may spread to larger areas in subsequent years. Because the jute fiber is extracted from the stem, stunted growth and short height would badly affect the fiber yield and quality. Ten symptomatic and ten asymptomatic healthy looking samples were collected from the field. Corchorus golden mosaic begomovirus is common in jute; therefore, all the samples were tested by PCR using JMFL-AF/JMFL-AR, DNA-A component specific primer pair and JMFL-BF/JMFL-BR, DNA-B component specific primer pairs (1). However, there was no amplification. Because the aphid Aphis gossypii was often noticed in the jute field, all the samples were tested by double-antibody sandwich (DAS)-ELISA for common aphid transmitted viruses, e.g., Cucumber mosaic virus, Bean common mosaic virus, Cowpea mosaic virus, Papaya ring spot virus, Potato leaf roll virus (PLRV), Potato virus Y, and Watermelon mosaic virus using commercial diagnostic kits (Agdia). The symptomatic samples showed positive reaction only for PLRV. Five ELISA-positive samples and five asymptomatic healthy samples were used for RNA extraction. Total RNA was extracted by using QIAGEN RNeasy mini kit. RT-PCR was carried out with PLRV CP gene specific primer pair (3) which generated a cDNA amplicon of 627 bp in all ELISA-positive symptomatic samples. PLRV was not detected in symptomless samples. The five purified cDNA products were cloned in a pGEM-T Easy vector (Promega) and were sequenced. One of the five identical sequences was deposited in GenBank (Accession No. KF233880). The consensus sequence was analyzed by NCBI BLAST and found to share 99% similarity with the coat protein sequence of PLRV reference strain (S77421). Nucleotide span and ORF finder (NCBI) analysis indicated the 627-bp PCR amplicon coded part of a coat protein gene that had 100% identity with translated gene product (Protein ID AAB33483). PLRV is a small isometric RNA virus with worldwide distribution belonging to the family Luteoviridae whose natural host range is mainly restricted to solanaceous plants and few plants of other families (2,4). To the best of our knowledge, this is the first report of PLRV naturally occurring on jute (C. olitorius). References: (1) R. Ghosh et al. J. Virol. Methods 159:34, 2009. (2) S. Guyader and D. G. Ducray. J. Gen. Virol. 83:1799, 2002. (3) M. A. Mayo et al. J. Gen. Virol. 70:1037, 1989. (4) K. Mukherjee et al. Virus Genes 26:247, 2003.

Biodiversity and full genome sequence of potato viruses Alfalfa mosaic virus and potato leaf roll virus in Egypt

2018 ◽  
Vol 73 (11-12) ◽  
pp. 423-438 ◽  
Author(s):  
Engy E. Abdel Aleem ◽  
Radwa M. Taha ◽  
Faiza A. Fattouh
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Leaf Roll ◽  
Alfalfa Mosaic Virus ◽  
Potato Leaf Roll Virus ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Potato Viruses

Abstract Solanum tuberosum (potato) is the second most important vegetable crop in Egypt. It is locally consumed, manufactured or supplied for export to Europe and other Arab countries. Potato is subject to infection by a number of plant viruses, which affect its yield and quality. Potato virus Y (PVY), potato leaf roll virus (PLRV), and Alfalfa mosaic virus (AMV) were detected in major potato-growing areas surveyed. Multiplex-RT-PCR assay was used for the detection of these three viruses in one reaction using three specific primer pairs designed to amplify genomic parts of each virus (1594 bp for PLRV, 795 bp for AMV, 801 bp for PVY). All three viruses were detected in a single reaction mixture in naturally infected field-grown potatoes. Multiplex RT-PCR improved sensitivity necessary for the early detection of infection. Incidence of single, double, or triple infection has been recorded in some locations. Full-length sequencing has been performed for an Egyptian FER isolate of PLRV. Through phylogenetic analysis, it was shown to occupy the same clade with isolate JokerMV10 from Germany. Complete nucleotide sequence of an Egyptian FER isolate of AMV and phylogenetic analysis was also performed; we propose that it is a new distinct strain of AMV belonging to a new subgroup IIC. This is the first complete nucleotide sequence of an Egyptian isolate of AMV. Genetic biodiversity of devastating potato viruses necessitates continuous monitoring of new genetic variants of such viruses.

scholarly journals First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing aphid

2020 ◽  
Author(s):  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
Jitesh Kumar ◽  
Tushar Ranjan
Keyword(s):  
Molecular Basis ◽  
Leaf Roll ◽  
Molecular Data ◽  
Combined Effect ◽  
Potato Leaf Roll Virus ◽  
Yield Losses ◽  
Potato Leaf ◽  
First Report ◽  
First Time

Abstract Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids population were found comparatively lower with maximum symptomatic plants, our molecular data further confirms the presence of PLRV in all possible symptomatic tissues such as tubers, shoots and leaves. For the first time, we have proposed molecular basis of aggravation of PLRV, where tuber acts as a reservoir during off-season and further transmitted by aphids.

scholarly journals First Report of the Presence of a Phytoplasma in Tomato in Mauritius

Plant Disease ◽  
1999 ◽  
Vol 83 (3) ◽  
pp. 304-304 ◽  
Author(s):  
A. Dookun ◽  
S. Aljanabi ◽  
S. Saumtally ◽  
L. J. C. Autrey
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Leaf Roll ◽  
Dna Amplification ◽  
Tomato Mosaic Virus ◽  
Universal Primers ◽  
Potato Leaf ◽  
Tomato Plants ◽  
First Report ◽  
Plant Dna

In recent years, tomato plants (Lycopersicon esculentum Mill., cultivar MST 32/1) grown in Mauritius were observed with symptoms characteristic of a phytoplasmal infection. Young plants exhibited stunted growth, with a bunchy top symptom on one or more stems. The leaves were small with a curled margin, sometimes were purplish, and were clustered as a rosette. On older plants, other leaf symptoms, such as necrotic vein, mosaic, and vein clearing, that were typical of viral infection were observed. In March to June 1998, research was initiated to determine whether a phytoplasma or a virus was associated with the problem. Virus screening was carried out by double antibody sandwich enzyme-linked immuno-sorbent assay (DAS-ELISA) with the following antisera: tomato mosaic virus (TMV), tomato leaf curl virus (TLCV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus X (PVX), potato virus Y (PVY), and potato leaf roll virus (PLRV). The presence of a phytoplasma was assayed by the polymerase chain reaction (PCR) with universal primers that amplify the 16S rDNA sequences. Oligonucleotide pairs RU3 and FU5 (1) and P1 and P6 (2) were evaluated separately. Template DNA was prepared from young leaves with the Nucleon Phyta-Pure Plant DNA extraction kit (catalog no. RPN 8511, Amersham Life Science, Buckinghamshire, U.K.). Amplification of a 880-bp or a 1,400-bp product with the two primer pairs confirmed the presence of a phyto-plasma in the tomato plants with the bunchy top symptoms. No amplification was obtained from plants that had both bunchy top and necrotic symptoms on the leaves and that were found to be infected with PVY by ELISA. To determine whether phenolics and other inhibitors from the virus-induced necrotic tissue interfered with the detection of phytoplasmas, plant DNA from these tissues was spiked with known phytoplasmal DNA. Amplification was not successful, confirming the presence of PCR inhibitors. Further purification of the DNA with phenol/chloroform before precipitation helped to obtain DNA of a purer quality from which a positive band representing the sequence of a phytoplasma was amplified. This is the first report of a phytoplasma in tomato in Mauritius. References: (1) U. Ahrens and E. Seemüller. Phytopathology 82:828, 1992. (2) S. Deng and D. Hiruku. J. Microbiol. Methods 14:53, 1991.

scholarly journals First Report on Molecular Basis of Potato Leaf Roll Virus Aggravation by Combined Effect of Tuber and Prevailing Aphid

2020 ◽  
Author(s):  
Ravi Ranjan Kumar ◽  
Md. Ansar ◽  
Kumari Rajani ◽  
Jitesh Kumar ◽  
Tushar Ranjan
Keyword(s):  
Molecular Basis ◽  
Yield Loss ◽  
Leaf Roll ◽  
Molecular Data ◽  
Combined Effect ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf ◽  
First Report ◽  
First Time

Abstract Objective The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield loss worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results Although population of aphids were found comparatively lower with maximum symptomatic plants, our molecular data further confirms the presence of PLRV in all possible symptomatic tissues such as tuber, shoot and leaf. For the first time, we have proposed molecular basis of aggravation of PLRV, where tuber acts as a reservoir during off-season and further transmitted by aphids.

scholarly journals In silico Characterization and Homology Modelling of Potato Leaf Roll Virus (PLRV) Coat Protein

2019 ◽  
pp. 1-8
Author(s):  
Jitesh Kumar ◽  
Tushar Ranjan ◽  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Homology Modelling ◽  
Leaf Roll ◽  
Potato Leaf Roll Virus ◽  
Z Score ◽  
Ramachandran Plot ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Crops

Polerovirus (Family-Luteoviridae) are one of the most destructive viruses causing detrimental diseases in vegetable crops in tropical regions of the world including India. Four species viz. potato leaf roll virus (PLRV), potato virus Y(PVY), potato virus X(PVX) and potato virus S(PVS) are known to cause different diseases in potato crops. Of the various viral diseases inflicting potato crops, potato leaf roll disease is the most destructive and widely distributed. They cause huge agro-economical losses (90%) worldwide and thus are the subjects of immense concern. PLRV is a phloem-limited spherical virus transmitted by several aphid species in a persistent manner. A study was performed in order to detect the infection of potato leaf roll virus from different regions of Bihar. These infected samples were diagnosed first using DAS-ELISA for the PLRV infection and later, coat protein was amplified and sequenced from PLRV positive sample. Phylogenetic tree deduced based on the nucleotide sequence of the coat protein gene gene showed a distinct divergence of PLRV isolates in two major clades. The molecular weight of the predicted protein sequence of 203 amino acids was found 22617.06 daltons while theoretical pI was 5.22. The extinction coefficient of predicted coat protein was 0.836. An attempt was taken in order to illustrate the 3D model of the coat protein which was further verified using Ramachandran plot. The model structure obtained using Swiss-Model had 92.9% residues in the most favourable region of the Ramachandran plot (Fig. 3c) and showed Z-score for bond angles, chi-1/chi-2 correlation and Ramachandran Z-score were 1.457, 1.773 and -2.633 respectively which exhibited  considerably good model quality.

Naturally occurring strains of the potato leaf roll virus

1951 ◽  
Vol 28 (7) ◽  
pp. 667-671 ◽  
Author(s):  
R. E. Webb ◽  
R. H. Larson ◽  
J. C. Walker
Keyword(s):  
Leaf Roll ◽  
Potato Leaf Roll Virus ◽  
Potato Leaf ◽  
Naturally Occurring

scholarly journals First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing aphid

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
Jitesh Kumar ◽  
Tushar Ranjan
Keyword(s):  
Molecular Basis ◽  
Leaf Roll ◽  
Molecular Data ◽  
Combined Effect ◽  
Potato Leaf Roll Virus ◽  
Yield Losses ◽  
Potato Leaf ◽  
First Report ◽  
First Time

Abstract Objective The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results Although aphids population were found comparatively lower with maximum symptomatic plants, our molecular data further confirms the presence of PLRV in all possible symptomatic tissues such as tubers, shoots and leaves. For the first time, we have proposed molecular basis of aggravation of PLRV, where tuber acts as a reservoir during off-season and further transmitted by aphids.

scholarly journals RT-PCR Based Cloning and Sequencing of Potato Leaf Roll Virus-Coat Protein (PLRV-CP) Gene for Characterization as a Bangladeshi PLRV Isolate and Its Phylogenetic Analysis

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Md. Belal Hossain ◽  
Tanjila Hasan ◽  
Ibne Siam Joy ◽  
Ratna Akter ◽  
Saleh Ahmed Shahriar
Keyword(s):  
Phylogenetic Analysis ◽  
Coat Protein ◽  
Leaf Roll ◽  
Protein Modeling ◽  
Potato Leaf Roll Virus ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Parsimony Method ◽  
Total Rna ◽  
Cp Gene

An experiment was conducted in Molecular Biology and Plant Virology Laboratory under the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. Total RNA was extracted from Potato leaf roll virus (PLRV) positive leaves and complementary DNA (cDNA) were synthesized from total RNA. Reverse transcriptase polymerase chain reaction (RT-PCR) based detection conditions were optimized by using coat protein (CP) gene specific primers. In PCR amplification cDNA and in nucleotide sequencing PCR product was used as a template. A 346 bp amplicon of PLRV- CP gene was amplified and amplified gene region was sequenced. The expected nucleotide sequence of amplified PLRV-CP gene showed 95 to 98% homology when compared with the isolates sequences reported in Gene Bank database. This explored novel PLRV-CP gene was characterized as a PLRV Bangladeshi isolate (Accession number, Bankit 2274496, MN605963). PLRV-CP gene protein modeling was carried out using Expert Protein Analysis System (ExPaSy), DNASTAR’s protein tools server used for 3D protein modeling. Phylogenetic analysis was also carried out, the tree was made by using MEGA 4.0 software and maximum parsimony method was selected to construct phylogenetic tree. The RT-PCR based molecular technique optimized in this study, would be a useful for early detection, epidemiological studies of PLRV as well as in seed tubers certification program and the novel hyper variable sequenced region of PLRV-CP gene will be useful in pathogen derived resistance breeding program against the PLRV local strain.

scholarly journals First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing aphid

2020 ◽  
Author(s):  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
Jitesh Kumar ◽  
Tushar Ranjan
Keyword(s):  
Molecular Basis ◽  
Leaf Roll ◽  
Molecular Data ◽  
Combined Effect ◽  
Potato Leaf Roll Virus ◽  
Yield Losses ◽  
Potato Leaf ◽  
First Report ◽  
First Time

Abstract Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids population were found comparatively lower with maximum symptomatic plants, our molecular data further confirms the presence of PLRV in all possible symptomatic tissues such as tubers, shoots and leaves. For the first time, we have proposed molecular basis of aggravation of PLRV, where tuber acts as a reservoir during off-season and further transmitted by aphids.

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