scholarly journals Characterization of Alternaria alternata Causing Black Spot Disease of Pomegranate in Israel Using a Molecular Marker

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1513-1518 ◽  
Author(s):  
Tami Gat ◽  
Orna Liarzi ◽  
Yulia Skovorodnikova ◽  
David Ezra
Keyword(s):  
Molecular Marker ◽  
Alternaria Alternata ◽  
Severe Disease ◽  
Outer Part ◽  
Black Spot ◽  
Spot Disease ◽  
Black Spots ◽  
Detached Leaves ◽  
Pathogenicity Tests ◽  
Black Spot Disease

Black spot disease of pomegranate is a relatively new disease in Israel that is caused by Alternaria alternata. The symptoms include black spots on leaves and fruit. Only the outer part of the fruit is damaged; the edible tissue remains unaffected. In this study, we obtained 50 isolates of A. alternata from infected pomegranate plants that were classified based on pathogenicity tests using detached leaves. Using an arbitrarily primed polymerase chain reaction, we identified one primer (primer CAG) that reacted only with DNA of isolates that induced the most severe disease symptoms. Based on the sequence of the amplified fragment, we generated a specific primer (primer C) that recognizes these highly virulent isolates. Therefore, we suggest that primer C can be utilized as a molecular marker for the detection of A. alternata isolates that cause black spot disease of pomegranate.

scholarly journals First Report of Alternaria Black Spot Disease Caused by Alternaria alternata on the Invasive Weed Solanum rostratum in Xinjiang, China

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 1021-1021
Author(s):  
J.-W. Guo ◽  
C.-X. Han ◽  
Y.-G. Zhang ◽  
Y.-X. Lu ◽  
H.-Y. Wang ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Black Spot ◽  
Invasive Weed ◽  
First Report ◽  
Spot Disease ◽  
Solanum Rostratum ◽  
Black Spot Disease

Investigation of Multiple Pathogens in Black-Spotted Dendrobium officinale Based on Culture-Independent and Dependent Methods

2020 ◽  
Vol 14 (2) ◽  
pp. 249-257
Author(s):  
Xiaoying Han ◽  
Yihong Dai ◽  
Miao Hu ◽  
Rong Xiao ◽  
Shuaishuai Zhang ◽  
...  
Keyword(s):  
Black Spot ◽  
Amplicon Sequencing ◽  
Dendrobium Officinale ◽  
Sequencing Data ◽  
Spot Disease ◽  
Culture Independent ◽  
Pathogenicity Tests ◽  
Multiple Pathogens ◽  
Black Spot Disease

Black spot disease which significantly reduces the quality of medicine plants is quite common in Dendrobium officinale fields. In this study, GXDF33, a strain of Alternaria, together with the reported pathogen Cladosporium, was isolated from symptomatic leaves. The in vitro pathogenicity tests on seedlings showed GXDF33 can cause black spot disease. Based on quantitative real-time polymerase chain reaction (qPCR) result, it was found that the biomass of both Cladosporium and Alternaria increased as the symptom went severer. According to amplicon sequencing data for the symptomatic samples from Guangxi and Zhejiang Provinces in China, Cladosporium and Alternaria were both common genera in symptomatic plants. To our knowledge, this is the first report unveiling the relationship between black spot disease of D. officinale and Cladosporium as well as Alternaria.

Postharvest chlorine treatments for the control of the persimmon black spot disease caused by Alternaria alternata

2001 ◽  
Vol 22 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Dov Prusky ◽  
Dani Eshel ◽  
Ilana Kobiler ◽  
Nir Yakoby ◽  
Delila Beno-Moualem ◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Black Spot ◽  
Spot Disease ◽  
Black Spot Disease

Impact de la maladie des points noirs sur la biologie du complexe Phoxinus (Cyprinidae) du lac Fortune, Québec

1994 ◽  
Vol 72 (9) ◽  
pp. 1611-1615 ◽  
Author(s):  
Anne Reine Paradis ◽  
François Chapleau
Keyword(s):  
Gonadosomatic Index ◽  
Black Spot ◽  
Condition Index ◽  
The Body ◽  
Spot Disease ◽  
Total Length ◽  
Predictive Parameters ◽  
Black Spots ◽  
Black Spot Disease

The purpose of this study was to quantify the effects of the black spot disease on growth, health, and fecundity of the fish and to define predictive parameters related to the infestation. Cyprinids belonging to the Phoxinus complex (P. eos, P. eos × P. neogaeus) of Lake Fortune (Gatineau Park, Québec, Canada) were sampled periodically between June and September 1992. The highest intensity of infestation was encountered in September (0–216 black spots; [Formula: see text], SD = 26.0). Our results indicate that: (i) the condition index of the Phoxinus complex and the gonadosomatic index in females are not affected by the level of infestation; (ii) age is a better predictive parameter of the number of black spots than total length; (iii) fins are significantly more infested than the body; and (iv) infestation occurs mainly between June and September. Our results suggest that the black spot disease does not influence the biology of fish belonging to the Phoxinus complex, even in instances of severe infestation.

scholarly journals The occurence of black spot disease in Astyanax aff. fasciatus(characiformes: characidae) in the Guaíba Lake basin, RS, Brazil

2014 ◽  
Vol 74 (3 suppl 1) ◽  
pp. S127-S134 ◽  
Author(s):  
F Flores-Lopes
Keyword(s):  
Low Frequency ◽  
Black Spot ◽  
Lake Basin ◽  
Body Parts ◽  
Spot Disease ◽  
Black Spots ◽  
Digenetic Trematodes ◽  
Fish Samples ◽  
High Prevalence ◽  
Black Spot Disease

Black spot disease is common in freshwater fish and is usually caused by the metacercaria stage of digenetic trematodes, normally from the Diplostomidae family. The present study evaluated the prevalence and intensity of this disease in Astyanax aff. fasciatus(Teleostei: Characiformes) in the Guaíba Lake basin (RS, Brazil), including body parts assessment and the points of sampling with higher occurrence of black spots. Fish samples were taken seasonally from December 2002 until October 2004. The samples were collected with the use of a seine net at eleven points. The specimens were fixed in 10% formalin and stored in 70% ethanol. Black spot disease showed a low frequency in the Guaíba lake basin (2.07%) and no specificity to the species Astyanax aff. fasciatus was observed. A high prevalence among the individuals and high intensity of infection levels was found in the ventral and dorsal regions in relation to other body parts (e.g., pectoral, pelvic and anal regions). Among the sampling points studied, we observed a higher prevalence on samples collected at points Gasômetro, Saco da Alemoa and Sinos, located in open areas with less occurrence of mollusks.

Effect of Modified Atmosphere for Control of Black Spot, Caused by Alternaria alternata, on Stored Persimmon Fruits

1997 ◽  
Vol 87 (2) ◽  
pp. 203-208 ◽  
Author(s):  
D. Prusky ◽  
A. Perez ◽  
Y. Zutkhi ◽  
R. Ben-Arie
Keyword(s):  
Alternaria Alternata ◽  
Modified Atmosphere Packaging ◽  
Black Spot ◽  
Inhibitory Effect ◽  
Modified Atmosphere ◽  
Inhibitory Effects ◽  
Spot Disease ◽  
Persimmon Fruit ◽  
Black Spot Disease

Modified atmosphere packaging (MAP) of persimmon fruit resulted in the accumulation of acetaldehyde to a level of 80 μg/ml; ethanol to a level of 900 μg/ml; and CO2 up to 30%. When fruits were stored at -1°C for 4 months in such atmospheres, the incidence of black spot disease, caused by Alternaria alternata, was reduced. The effects of each of these gases were examined to determine their individual involvement in the inhibition of Alternaria development during storage. When A. alternata, grown at 20°C on potato dextrose agar or inoculated in persimmon fruit, was exposed for 24 h to different levels of each volatile, acetaldehyde was the most fungistatic but only at concentrations higher than those that accumulated under MAP; CO2 was moderately inhibitory at concentrations from 10 to 60%, whereas ethanol had no effect. Similar inhibitory effects were obtained with acetaldehyde at 620 μg/ml or 30% CO2 when in vitro cultures of A. alternata and infected fruits were exposed for up to 2 weeks at 20°C, but 1,000 μg of ethanol per ml had only a transitory inhibitory effect under these conditions. Based on analysis of the effect of concentration versus time for each gas accumulating in MAP, we suggest that the increasing concentration of CO2 during storage is the principal factor in the inhibition of black spot disease development.

scholarly journals First Report of Black Spot Disease Caused by Alternaria alternata on Sweet Persimmon Fruits

Mycobiology ◽  
2013 ◽  
Vol 41 (3) ◽  
pp. 167-169 ◽  
Author(s):  
Jung Han Lee ◽  
Jinwoo Kim ◽  
Youn-Sig Kwak
Keyword(s):  
Alternaria Alternata ◽  
Black Spot ◽  
First Report ◽  
Spot Disease ◽  
Black Spot Disease
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